##gff-version 3
##sequence-region BBa_K525411 1 4854
BBa_K525411	.	operator	20	44	.	+	0	ID=annotation2161268;Name=lac operator
BBa_K525411	.	engineered_region	3199	4854	.	+	0	ID=annotation2161276;Name=K525999
BBa_K525411	.	ribosome_entry_site	56	59	.	+	0	ID=annotation2161270;Name=RBS
BBa_K525411	.	promoter	1	20	.	+	0	ID=annotation2161267;Name=T7 promoter
BBa_K525411	.	sequence_feature	3193	3198	.	+	0	ID=annotation2161274;Name=Freiburg scar
BBa_K525411	.	stop_codon	4852	4854	.	+	0	ID=annotation2161278;Name=TAA
BBa_K525411	.	non_covalent_binding_site	76	81	.	+	0	ID=annotation2161279;Name=NgoMIV
BBa_K525411	.	CDS	3199	4854	.	+	0	ID=annotation2161275;Name=Luciferase
BBa_K525411	.	engineered_region	53	64	.	+	0	ID=annotation2161269;Name=B0034
BBa_K525411	.	engineered_region	73	3192	.	+	0	ID=annotation2161271;Name=K525401
BBa_K525411	.	CDS	73	3192	.	+	0	ID=annotation2161272;Name=SbpA domain
BBa_K525411	.	start_codon	73	75	.	+	0	ID=annotation2161273;Name=ATG
BBa_K525411	.	non_covalent_binding_site	4846	4851	.	+	0	ID=annotation2161277;Name=AgeI
>BBa_K525411
taatacgactcactataggggaattgtgagcggataacaattcctactagagaaagaggagaaatactagagatggccg
gcgctcaggttaacgactacaacaagatctccggctacgcaaaggaggctgttcagtccctcgtcgaccagggtgttat
ccagggcgataccaacggcaacttcaacccactgaacaccgttactcgcgcacaggctgctgagatcttcaccaaggct
ctcgaactggaggcagacggtgacgtcaacttctccgatgttaagaagggcgcttggtactacaactctatcgcagctg
ttgtcgcaaacggcatcttcgagggtgtgtccgctaacgagttcgcaccaaacaagtcccttacccgttccgaggctgc
aaaggttctcgttgacgctttcggcctggagggctctgaatccctctcccagttcgcagacgcttcccaggtcaagggt
tgggcaaagtctgctctggagaccgcagttgctaacggcatcttcactggctccgaggaaaacggtaagcttaacctca
agcctaacgcagctatcacccgccaggatttcgcagttgtcttcgctcgtaccctggacctcgcagttgagaccgttga
cgcttccgtcaaggcaatcaacaacactaccgtggaggttaccttcgatgaagaggttgacaacgtcgaggctctgaag
ttcaagatcgaaggccttgaggttaagaacgcagctgttaagcagaccaacaagaaggtcgttgttctcactaccgagg
cacagaccgctgacaaggaatacgtcgtgaccctggatggcgaggagatcggtaagttcaagggcatcgaagcagttgt
tccaaagtccatcgtcctcaagactaccaacacccagggcaaggttggtaaccaggttaccctgactgctgacgtcggc
gttaaggcagctggcatcccagttaccttcaacgtcgacgcacctaccggctctcttaacaaggatgctgtggttgagg
tttacaccaacgcagagggcatcgcttcctactcctacactcagtacgcaccagacgctgacgatgtcaccgtttaccc
aaccggcgcacctcagctccgcgctttcggtccagtttactggggcgtcgacaacatcctgaccatcgaagaggttact
accggcaacaccctcgcaaacggtgttaagaagacctacaaggtcactttcaaggacccaaagaccggcgctgcactga
ccaacaagaagcttaacgtgtccttcgttgagaacaccaacgttgctttcaacgcaatctctaaggctactgtcaccaa
cccttcctccggcctcaccgttaccccataccagactaccaccggcctccaggaagagatccaggttaccactgattcc
aacggccaggcaaccttcgtcgtttctggcaccaacaccgctgttactccatacgtcttcgtggacggttcctcctccg
ttctcggcgtttctaccgtcaccggcaccaacaacgttactcaggcaaccaccgctaacaagaagtgggaggcaaccga
actgactgctaccgcagctcaggttaagttcgagggtgcacagcttaaccaccagatcaccgtcgagcgtgacggcgaa
gaggaggcagcagctcagtacggctccaagctcaacggtcgcgaatacaaggttaaggttctggataaggacggcaagc
cttacgcaaacggcctcgtcaacatcggtctggacgaggtgcttgatcgtaacctcaacaccaactccaaggctcagtt
cgcaaacgttactgacggcaccgctctgaccctcaacccaggcacctcccagcagggtaagatcaagctgaactctaag
ggcgaggcaactgttgtcctttacggcgctaagggtgaagttggcaccccagttgtctggatcgaccagaacacctccc
agaacaaccagtccggcgttctcgaggatggtgagcctttcttcaaggcaccagtttccaacttccagtacgaacgcgt
catgggcgctgagttcaccgtggagggcaagacttctaaccagaacgttggtaccgacggcatcgcaaactacgctttc
gcactgaccaaccagtccggcaagaccctcactaacctgatcggtaagatgacctacgaaatccgtaacacctccggcc
agccaatcgaggttaagacccctatctcctaccgcgaccagaacaacgtctcttccactcttttcaagatcgaggaata
cggctccatcaccatctccgctgatgttatctctacctccaacgcagctttcaccatcgagactgcaccaaacggtaag
gctgcaaccgttgtcgttaccccatccttcgttaccgctaacaacttcgacgcatccgctgacaacaacctcaacggcg
tcgtgactgatgcacgtgacttcaacaagcacgttaccgctcctgcactgaccgctaccttcggcaacaacaaggaggt
tggtactaactacaccggcaccgtccagtacttcaacaaggacaagaagaccatcactttcaacggcaagaacgcagtt
aagtacgctggtgaatctggcaagacctacaagtacttcggcctcggttccaccccaatctccaccgcagatgacttca
tcactcagctccagaacaacgctgcaaacggctccgttaccgtcacctacaaggttgttgacaacgaggtccagttcta
catcgtgtctttcaacaacgatggcgctgcaaacgctgttaagccagagcctaccgcagttgctccaactgacggtacc
gtcgcattcaccaacacctccttcaacgcttccgcaaaccagactatcaccgttaccgacgctgatcttaacgcagacg
ctaccgttgcagaaactaccaccgtcaaggttacctccggcactgttgtctacaccctcaccgctaccgagactggcgt
gaacaccggcgttttcaccgcaaccctgactgctgcacagctcgctgttctgtctgacggcgtcatcaccgcaacctac
gaggatgttaagaacaccactggctcctccgttacccgcaccgctaccgcaactcttaacaccgtcgttggtcaggtta
actacaacgcagcagctaccaccgcatactccaccggcgaagacgccaaaaacataaagaaaggcccggcgccattcta
tccgctggaagatggaaccgctggagagcaactgcataaggctatgaagagatacgccctggttcctggaacaattgct
tttacagatgcacatatcgaggtggacatcacttacgctgagtacttcgaaatgtccgttcggttggcagaagctatga
aacgatatgggctgaatacaaatcacagaatcgtcgtatgcagtgaaaactctcttcaattctttatgccggtgttggg
cgcgttatttatcggagttgcagttgcgcccgcgaacgacatttataatgaacgtgaattgctcaacagtatgggcatt
tcgcagcctaccgtggtgttcgtttccaaaaaggggttgcaaaaaattttgaacgtgcaaaaaaagctcccaatcatcc
aaaaaattattatcatggattctaaaacggattaccagggatttcagtcgatgtacacgttcgtcacatctcatctacc
tcccggttttaatgaatacgattttgtgccagagtccttcgatagggacaagacaattgcactgatcatgaactcctct
ggatctactggtctgcctaaaggtgtcgctctgcctcatagaactgcctgcgtgagattctcgcatgccagagatccta
tttttggcaatcaaatcattccggatactgcgattttaagtgttgttccattccatcacggttttggaatgtttactac
actcggatatttgatatgtggatttcgagtcgtcttaatgtatagatttgaagaagagctgtttctgaggagccttcag
gattacaagattcaaagtgcgctgctggtgccaaccctattctccttcttcgccaaaagcactctgattgacaaatacg
atttatctaatttacacgaaattgcttctggtggcgctcccctctctaaggaagtcggggaagcggttgccaagaggtt
ccatctgccaggtatcaggcaaggatatgggctcactgagactacatcagctattctgattacacccgagggggatgat
aaaccgggcgcggtcggtaaagttgttccattttttgaagcgaaggttgtggatctggataccgggaaaacgctgggcg
ttaatcaaagaggcgaactgtgtgtgagaggtcctatgattatgtccggttatgtaaacaatccggaagcgaccaacgc
cttgattgacaaggatggatggctacattctggagacatagcttactgggacgaagacgaacacttcttcatcgttgac
cgcctgaagtctctgattaagtacaaaggctatcaggtggctcccgctgaattggaatccatcttgctccaacacccca
acatcttcgacgcaggtgtcgcaggtcttcccgacgatgacgccggtgaacttcccgccgccgttgttgttttggagca
cggaaagacgatgacggaaaaagagatcgtggattacgtcgccagtcaagtaacaaccgcgaaaaagttgcgcggagga
gttgtgtttgtggacgaagtaccgaaaggtcttaccggaaaactcgacgcaagaaaaatcagagagatcctcataaagg
ccaagaagggcggaaagatcgccgtgaccggttaa