LOCUS       BBa_K525562             2410 bp    DNA     linear       23-Dec-2024
DEFINITION  Fusion protein of NADP+ Oxidoreductase and BisdA and BisdB with 
            middle strong promoter,RBS
ACCESSION   BBa_K525562
VERSION     BBa_K525562.1  
FEATURES             Location/Qualifiers
     misc_feature    2402..2407
                     /label=AgeI
     misc_feature    2408..2410
                     /label=TAA
     misc_feature    65..70
                     /label=NgoMIV
     misc_feature    62..64
                     /label=ATG
     promoter        1..35
                     /label=promoter
     misc_feature    44..55
                     /label=B0034
     misc_feature    62..811
                     /label=K525499
     misc_feature    818..1128
                     /label=K123000
     misc_feature    1135..2410
                     /label=K123001
     misc_feature    1129..1134
                     /label=Freiburg Scar
     misc_feature    812..817
                     /label=Freiburg Scar
     CDS             818..1128
                     /label=BisdA
     CDS             1135..2410
                     /label=BisdB
     CDS             162..811
                     /label=Reductase
     RBS             47..50
                     /label=RBS
ORIGIN
        1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaatacta
       61 gatggccggc gctgattggg taacaggcaa agtcactaaa gtgcagaact ggaccgacgc
      121 cctgtttagt ctcaccgttc acgcccccgt gcttccgttt accgccgggc aatttaccaa
      181 gcttggcctt gaaatcgacg gcgaacgcgt ccagcgcgcc tactcctatg taaactcgcc
      241 cgataatccc gatctggagt tttacctggt caccgtcccc gatggcaaat taagcccacg
      301 actggcggca ctgaaaccag gcgatgaagt gcaggtggtt agcgaagcgg caggattctt
      361 tgtgctcgat gaagtgccgc actgcgaaac gctatggatg ctggcaacgg gcacagcgat
      421 tggcccttat ttatcgattc tgcaactagg taaagattta gatcgcttca aaaatctggt
      481 cctggtgcac gccgcacgtt atgccgccga cttaagctat ttgccactga tgcaggaact
      541 ggaaaaacgc tacgaaggaa aactgcgcat tcagacggtg gtcagtcggg aaacggcagc
      601 ggggtcgctc accggacgga taccggcatt aattgaaagt ggggaactgg aaagcacgat
      661 tggcctgccg atgaataaag aaaccagcca tgtgatgctg tgcggcaatc cacagatggt
      721 gcgcgataca caacagttgc tgaaagagac ccggcagatg acgaaacatt tacgtcgccg
      781 accgggccat atgacagcgg agcattactg gaccggccct catatccaag ttacgactcg
      841 tgatggtgaa attcgcgagc tggacgttgc agcttccggt ttcctgatgg aagcgctgcg
      901 tgacgctaac atcgatggtg tggaggcgat gtgtggcggt tgctgttctt gcgcgacttg
      961 ccacgtctac attgacgcgg ctccagccgg taccctgccg ccggtgtcta gcgatgaaga
     1021 aatgctgctg tccggcctgg tttctacccc gggccgctct cgtctgtctt gccagatccc
     1081 ggtaaccgca gaactggacg gcctgaaact gaccatcccg ccggatagca ccggcaaccc
     1141 acaaactctg ccggtatttc cagacctgga tatcttcagc ccggaatacg cttgtaaccg
     1201 cgaaaaatat gcagcgcgtg cgctgcgcga ttacccgctg cacttttata aaccgctgaa
     1261 cctgtggatt gtctccaaac ataaagacgt tcgtagcgcg ctgttcaccc cgcaggtctt
     1321 cagctccgta gcttttggtc tgctgcctcc accagacgat atcgctcctc gcgtgccgga
     1381 cctgtacacc gacgttcatc tgccgtccat ggatccgccg gagcacacca aactgcgtgt
     1441 tccggtgcag caggctctgc tgccgggccg tctggtaggc aaagacgagg ttgtgcgccg
     1501 tattgcgaac gaactgatcg atacttttat cgacaaaggc gaatgcgatc tgctgcatga
     1561 tttctcttat aagctggcgc tgtacctgat tgtagacctg ctgggtatcc ctaaagaacg
     1621 tgcggaggac taccaccgct ggtccaactg tttcttccag ctgttcacgc cgaaggttcc
     1681 tgaacgtgcg gatgctcgtt tctttgtacc gatgccggag gaagtgctgc gccagaactg
     1741 ggaaggtctg gccgaagcaa atgactatct gcgtgaagtt gttgaaaacc tggaccgtaa
     1801 tccgggcaac aacatgctgt ctaacctgct gcaactgcgt gaaccggatg gctctcgtac
     1861 tatcactatc tccgcgaacg tttgcaacgc cctggaattt ggcgcggcgg gtcacgatac
     1921 gaccgcaacc ctgatcgctc acctgactta tttcgtgctg accaccccgg acctgaagga
     1981 cactctgacc gaagatccat ctctgattcc ggcagccatc tctgaaaccc tgcgtcgtcg
     2041 ttgcccggtt gacggtctgt tccgccgcac cctgagcgac gtagaactgt gcggtcagaa
     2101 gatcgaatcc ggttctatcg tgtacctgga cctgaccgcc gctaacctgg atccggacgt
     2161 tttcccggag ccggagactt tccgtctgaa ccgcgataac attaaagaaa tggtgtcttt
     2221 cggcttcggt cgtcacgtct gtgcaggtca gtacctgagc cgcatcgaag ctaaagcagc
     2281 atacgaagaa ctgatgcgtc gtattcctaa tatgcgtctg gcagatggct tcaaactgga
     2341 gtacatgccg agcgtggcca ccactgttct gaaaggcctg ccgctggttt gggataaaaa
     2401 caccggttaa
//