LOCUS       BBa_K525710             2064 bp    DNA     linear       22-Dec-2024
DEFINITION  DNA ligase from Escherichia coli (LigA) with PT7, RBS and His-tag
ACCESSION   BBa_K525710
VERSION     BBa_K525710.1  
FEATURES             Location/Qualifiers
     promoter        1..20
                     /label=T7 promoter
     misc_feature    21..33
                     /label=B0034
     RBS             24..27
                     /label=RBS
     misc_feature    34..36
                     /label=ATG
     CDS             34..2045
                     /label=LigA
     misc_feature    2047..2064
                     /label=His-tag
ORIGIN
        1 taatacgact cactataggg aaagaggaga aaaatggaat caatcgaaca acaactgaca
       61 gaactgcgaa cgacgcttcg ccatcatgaa tatctttatc atgtgatgga tgcgccggaa
      121 attcccgacg ctgaatacga caggctgatg cgcgaactgc gcgagctgga aaccaaacat
      181 ccagaactga ttacgcctga ttcgcctact caacgtgtag gcgctgcgcc gctggcggct
      241 ttcagccaga tacgccatga agtaccaatg ctgtcactgg ataacgtttt tgatgaagaa
      301 agctttcttg ctttcaacaa acgtgtgcag gaccgtctga aaaacaacga gaaagtcacc
      361 tggtgctgtg agctgaagct ggatggtctt gccgtcagta ttctgtatga aaatggcgtt
      421 ttagtcagtg ccgcgacccg tggcgatggc accaccgggg aagatatcac gtctaatgtg
      481 cgtactattc gcgccattcc gctgaagctg cacggagaga atatcccggc gcgtctggaa
      541 gtgcgtggtg aagtgttcct gccgcaggcg gggttcgaaa agattaacga agatgcgcga
      601 cgcacgggcg ggaaagtgtt tgctaaccca cgtaatgcgg cagctggttc actgcgtcag
      661 cttgatccgc gtattacagc gaagcgaccg ctcacttttt tctgctatgg cgttggtgtt
      721 ctggaaggtg gcgagctgcc ggatactcat cttggccgtt tactgcaatt taaaaagtgg
      781 gggttgccgg tcagcgatcg ggtaacgctt tgtgaatcgg cggaagaagt gctggcgttc
      841 tatcacaaag tggaagaaga ccgcccgacg ctgggctttg atatcgacgg cgtggtgatt
      901 aaggtcaact cactggcaca gcaggagcag cttggctttg tcgcgcgtgc cccgcgctgg
      961 gcggtagcgt ttaaattccc ggcgcaggag cagatgacct ttgtgcgtga cgtcgagttt
     1021 caggttgggc gtactggcgc gattacgcct gttgcgcgtc tggaacctgt ccatgttgca
     1081 ggcgtgctgg tgagtaacgc aaccttacac aatgcggatg aaatcgaacg tcttggttta
     1141 cgcattggcg ataaagtggt gattcgccgc gctggcgacg tgatcccgca ggtggttaac
     1201 gtcgtgcttt ctgaacgccc ggaagatacc cgtgaggttg tattcccgac gcattgtccg
     1261 gtatgtggtt ctgacgttga gcgtgtggaa ggtgaagcgg ttgcccgctg taccggcggc
     1321 ctgatttgcg gtgcgcagcg taaagagtcg ctgaaacact ttgtttcccg ccgtgcgatg
     1381 gatgttgacg gaatgggcga caaaatcatc gatcagctgg ttgaaaaaga atatgtccat
     1441 actccggcag atctgttcaa actcaccgca ggcaaactga ccggactgga gcgtatgggg
     1501 ccaaaatcgg cacaaaacgt ggttaacgcg ctggaaaaag cgaaagaaac cacctttgct
     1561 cgcttcctct atgcacttgg catccgtgaa gtcggcgagg ccaccgcagc aggtctggcg
     1621 gcatatttcg gcacgctgga agcgctggaa gccgcttcga ttgaagagct gcaaaaggtg
     1681 cctgatgttg gcattgtcgt tgcatcccac gttcacaact tctttgccga agaaagcaac
     1741 cgcaatgtca tcagcgagct gttggcggaa ggtgttcact ggcctgcgcc gatcgttatc
     1801 aacgcggaag agattgacag cccgtttgct ggtaaaaccg tggtgcttac gggcagctta
     1861 agccagatgt cgcgtgatga cgctaaagct cgactggtcg aactgggcgc gaaagtcgcg
     1921 ggcagcgtgt cgaagaaaac cgatctggtg atagcgggtg aagcggcagg atctaaactg
     1981 gcgaaggcgc aggaactggg cattgaagtc atcgacgaag cggaaatgct gcgtttgctg
     2041 ggtagccatc atcatcatca tcat
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