##gff-version 3
##sequence-region BBa_K525710 1 2064
BBa_K525710	.	promoter	1	20	.	+	0	ID=annotation2161299;Name=T7 promoter
BBa_K525710	.	CDS	34	2045	.	+	0	ID=annotation2161303;Name=LigA
BBa_K525710	.	ribosome_entry_site	24	27	.	+	0	ID=annotation2161301;Name=RBS
BBa_K525710	.	engineered_region	21	33	.	+	0	ID=annotation2161300;Name=B0034
BBa_K525710	.	start_codon	34	36	.	+	0	ID=annotation2161302;Name=ATG
BBa_K525710	.	tag	2047	2064	.	+	0	ID=annotation2161304;Name=His-tag
>BBa_K525710
taatacgactcactatagggaaagaggagaaaaatggaatcaatcgaacaacaactgacagaactgcgaacgacgcttc
gccatcatgaatatctttatcatgtgatggatgcgccggaaattcccgacgctgaatacgacaggctgatgcgcgaact
gcgcgagctggaaaccaaacatccagaactgattacgcctgattcgcctactcaacgtgtaggcgctgcgccgctggcg
gctttcagccagatacgccatgaagtaccaatgctgtcactggataacgtttttgatgaagaaagctttcttgctttca
acaaacgtgtgcaggaccgtctgaaaaacaacgagaaagtcacctggtgctgtgagctgaagctggatggtcttgccgt
cagtattctgtatgaaaatggcgttttagtcagtgccgcgacccgtggcgatggcaccaccggggaagatatcacgtct
aatgtgcgtactattcgcgccattccgctgaagctgcacggagagaatatcccggcgcgtctggaagtgcgtggtgaag
tgttcctgccgcaggcggggttcgaaaagattaacgaagatgcgcgacgcacgggcgggaaagtgtttgctaacccacg
taatgcggcagctggttcactgcgtcagcttgatccgcgtattacagcgaagcgaccgctcacttttttctgctatggc
gttggtgttctggaaggtggcgagctgccggatactcatcttggccgtttactgcaatttaaaaagtgggggttgccgg
tcagcgatcgggtaacgctttgtgaatcggcggaagaagtgctggcgttctatcacaaagtggaagaagaccgcccgac
gctgggctttgatatcgacggcgtggtgattaaggtcaactcactggcacagcaggagcagcttggctttgtcgcgcgt
gccccgcgctgggcggtagcgtttaaattcccggcgcaggagcagatgacctttgtgcgtgacgtcgagtttcaggttg
ggcgtactggcgcgattacgcctgttgcgcgtctggaacctgtccatgttgcaggcgtgctggtgagtaacgcaacctt
acacaatgcggatgaaatcgaacgtcttggtttacgcattggcgataaagtggtgattcgccgcgctggcgacgtgatc
ccgcaggtggttaacgtcgtgctttctgaacgcccggaagatacccgtgaggttgtattcccgacgcattgtccggtat
gtggttctgacgttgagcgtgtggaaggtgaagcggttgcccgctgtaccggcggcctgatttgcggtgcgcagcgtaa
agagtcgctgaaacactttgtttcccgccgtgcgatggatgttgacggaatgggcgacaaaatcatcgatcagctggtt
gaaaaagaatatgtccatactccggcagatctgttcaaactcaccgcaggcaaactgaccggactggagcgtatggggc
caaaatcggcacaaaacgtggttaacgcgctggaaaaagcgaaagaaaccacctttgctcgcttcctctatgcacttgg
catccgtgaagtcggcgaggccaccgcagcaggtctggcggcatatttcggcacgctggaagcgctggaagccgcttcg
attgaagagctgcaaaaggtgcctgatgttggcattgtcgttgcatcccacgttcacaacttctttgccgaagaaagca
accgcaatgtcatcagcgagctgttggcggaaggtgttcactggcctgcgccgatcgttatcaacgcggaagagattga
cagcccgtttgctggtaaaaccgtggtgcttacgggcagcttaagccagatgtcgcgtgatgacgctaaagctcgactg
gtcgaactgggcgcgaaagtcgcgggcagcgtgtcgaagaaaaccgatctggtgatagcgggtgaagcggcaggatcta
aactggcgaaggcgcaggaactgggcattgaagtcatcgacgaagcggaaatgctgcgtttgctgggtagccatcatca
tcatcatcat