LOCUS       BBa_K525740             2037 bp    DNA     linear       25-Nov-2024
DEFINITION  DNA ligase from Escherichia coli (LigA) with His-tag
ACCESSION   BBa_K525740
VERSION     BBa_K525740.1  
FEATURES             Location/Qualifiers
     misc_feature    4..9
                     /label=NgoMIV
     misc_feature    1..3
                     /label=ATG
     CDS             1..2018
                     /label=LigA
     misc_feature    2020..2037
                     /label=His-tag
ORIGIN
        1 atggccggcg aatcaatcga acaacaactg acagaactgc gaacgacgct tcgccatcat
       61 gaatatcttt atcatgtgat ggatgcgccg gaaattcccg acgctgaata cgacaggctg
      121 atgcgcgaac tgcgcgagct ggaaaccaaa catccagaac tgattacgcc tgattcgcct
      181 actcaacgtg taggcgctgc gccgctggcg gctttcagcc agatacgcca tgaagtacca
      241 atgctgtcac tggataacgt ttttgatgaa gaaagctttc ttgctttcaa caaacgtgtg
      301 caggaccgtc tgaaaaacaa cgagaaagtc acctggtgct gtgagctgaa gctggatggt
      361 cttgccgtca gtattctgta tgaaaatggc gttttagtca gtgccgcgac ccgtggcgat
      421 ggcaccaccg gggaagatat cacgtctaat gtgcgtacta ttcgcgccat tccgctgaag
      481 ctgcacggag agaatatccc ggcgcgtctg gaagtgcgtg gtgaagtgtt cctgccgcag
      541 gcggggttcg aaaagattaa cgaagatgcg cgacgcacgg gcgggaaagt gtttgctaac
      601 ccacgtaatg cggcagctgg ttcactgcgt cagcttgatc cgcgtattac agcgaagcga
      661 ccgctcactt ttttctgcta tggcgttggt gttctggaag gtggcgagct gccggatact
      721 catcttggcc gtttactgca atttaaaaag tgggggttgc cggtcagcga tcgggtaacg
      781 ctttgtgaat cggcggaaga agtgctggcg ttctatcaca aagtggaaga agaccgcccg
      841 acgctgggct ttgatatcga cggcgtggtg attaaggtca actcactggc acagcaggag
      901 cagcttggct ttgtcgcgcg tgccccgcgc tgggcggtag cgtttaaatt cccggcgcag
      961 gagcagatga cctttgtgcg tgacgtcgag tttcaggttg ggcgtactgg cgcgattacg
     1021 cctgttgcgc gtctggaacc tgtccatgtt gcaggcgtgc tggtgagtaa cgcaacctta
     1081 cacaatgcgg atgaaatcga acgtcttggt ttacgcattg gcgataaagt ggtgattcgc
     1141 cgcgctggcg acgtgatccc gcaggtggtt aacgtcgtgc tttctgaacg cccggaagat
     1201 acccgtgagg ttgtattccc gacgcattgt ccggtatgtg gttctgacgt tgagcgtgtg
     1261 gaaggtgaag cggttgcccg ctgtaccggc ggcctgattt gcggtgcgca gcgtaaagag
     1321 tcgctgaaac actttgtttc ccgccgtgcg atggatgttg acggaatggg cgacaaaatc
     1381 atcgatcagc tggttgaaaa agaatatgtc catactccgg cagatctgtt caaactcacc
     1441 gcaggcaaac tgaccggact ggagcgtatg gggccaaaat cggcacaaaa cgtggttaac
     1501 gcgctggaaa aagcgaaaga aaccaccttt gctcgcttcc tctatgcact tggcatccgt
     1561 gaagtcggcg aggccaccgc agcaggtctg gcggcatatt tcggcacgct ggaagcgctg
     1621 gaagccgctt cgattgaaga gctgcaaaag gtgcctgatg ttggcattgt cgttgcatcc
     1681 cacgttcaca acttctttgc cgaagaaagc aaccgcaatg tcatcagcga gctgttggcg
     1741 gaaggtgttc actggcctgc gccgatcgtt atcaacgcgg aagagattga cagcccgttt
     1801 gctggtaaaa ccgtggtgct tacgggcagc ttaagccaga tgtcgcgtga tgacgctaaa
     1861 gctcgactgg tcgaactggg cgcgaaagtc gcgggcagcg tgtcgaagaa aaccgatctg
     1921 gtgatagcgg gtgaagcggc aggatctaaa ctggcgaagg cgcaggaact gggcattgaa
     1981 gtcatcgacg aagcggaaat gctgcgtttg ctgggtagcc atcatcatca tcatcat
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