BBa_K526001 1 BBa_K526001 ptrc*-Dpn 2011-09-25T11:00:00Z 2015-05-08T01:12:35Z http://www.ncbi.nlm.nih.gov/nuccore/25307955?from=1637214&to=1637675 http://www.ncbi.nlm.nih.gov/nuccore/25307955?from=1637672&to=1638436 ptrc* is a modified form of ptrc promoter with the -35 and -10 regions being present in the reverse order and the promoter is flanked by fimE recognition sequence. FimE is an invertase capable of inverting any DNA sequence flanked between its recognition seqence. This fim inversion system would help avoid leaky expression. We expressed a new lysis device, DpnI under the control of fim inversion promoter. DpnI is a restriction enzyme capable of chopping up all methylated DNAs. We propose DpnI as a novel lysis device. DpnC and DpnD are obtained from Streptococcus pneumoniae R6 strain. false false _692_ 0 8432 9 It's complicated true One should be careful while expressing Dpn genes. Dpn is supposed to chop up the DNA and cause cell death. Hence, no leaky level of Dpn is needed to recover live cells false Sung Kuk Lee annotation2150194 1 fim inversion promoter range2150194 1 1 220 annotation2150193 1 DpnC range2150193 1 221 1046 annotation2150195 1 DpnD range2150195 1 1077 1536 BBa_K526001_sequence 1 attgttatctttatttatagatgtttatattgcatgaggtggtttttggagagaagaatgaggaagatgcgtcgagccacagaaacgttagctttacatatagcggaggtgatgtgaaattaatttacaatagaaataatttacatatcaaacagttagatgctttttgtcgttttttaatatttttatggttgacaattaatcatccggctcgtataatatggacagtttggccccaaatgtttcatctttccatggtactagagtcacacaggacggccggatggaattacactttaatttagaattagtagaaacctataaaagtaatagccaaaaggcacgtatcttaacggaggattgggtttatcgtcagtcttattgccctaattgtgggaacaatcccttaaatcattttgaaaataatcggcctgtagcagatttttactgtaatcattgtagtgaggagtttgaactaaagagcaaaaaaggaaatttttcatcaacaatcaatgatggtgcttatgcaacgatgatgaagcgtgtgcaggcagataataatcctaatttcttttttttaacttacacaaaaaattttgaggtaaataactttcttgtccttccgaagcaatttgttacaccgaaatcgattattcaaagaaaaccacttgcaccaactgctagacgagcaggttggattggttgtaacattgatttatcacaagtaccttctaaaggaaggatatttcttgtgcaagatggacaagttagagatccagaaaaagttacaaaagaatttaagcaaggtttatttttaaggaagagctctctgtcatcaagaggttggacaatagaaattctaaattgtatagataagatagagggttcagaatttacccttgaagatatgtatcgttttgaaagtgacctaaaaaatatctttgttaagaacaatcatatcaaagaaaagattaggcaacagcttcaaatattaagagacaaagaaataatagaatttaaaggtagaggaaagtatcggaaattataataatactagagtcacacaggacggccggatgaaaacgaaacaacttgttgcatcagaagaggtgtatgatttcttaaaagtcatctggcctgattatgaaactgaaagccgttacgataacctaagtttaatcgtctgtaccttatcagatcccgattgtgtgagatggttatctgaaaatatgaaatttggtgacgaaaaacaactagctttgatgaaggaaaaatatgggtgggaagtaggagataaattgccagagtggctacatagctcctatcatagattattgttaataggtgaattattggaaagcaatctaaaactgaaaaagtatacagtagaaattacagaaactttatcacgtttagtaagtatagaggctgaaaatccagatgaagccgaacgacttgtaagagaaaagtataagagttgtgaaattgttcttgatgcagatgattttcaggactatgacactagcatatatgaataataagctagcttgtt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z