BBa_K527000 1 BBa_K527000 Biosynthesis/Oximes/CYP79A2 2011-09-16T11:00:00Z 2015-05-08T01:12:35Z Institute of Plant Biology and Biotechnology Faaculty og LIFE Science Copenhagen University Denmark This BioBrick BBa_K527000 encode the plant enzyme Cytochrome P450 CYP79A2. Cytochromes p450 are defined as heme-thiolate proteins featuring a particular spectral signature at 450 nm, thus the name. Despite the label as cytochromes, these proteins are not involved in electron transfers, but act as monooxygenases in a wide range of reactions such as epoxidation, N-dealkylation, O-dealkylation, S-oxidation and hydroxylation. CYP79A2 is an N-hydroxylase converting L-phenylalanine into phenylacetaldoxime, the precursor of the cyanogenic glycoside benzylglucosinolate. Oximes as the phenylacetaldoxime is generally toxic to fungi and is frequently used as chemical fungicides. They function by inhibiting mitochondrial oxidases and thereby promote lipid peroxidation and the production of toxic reactive oxygen species. false true _693_ 0 8631 9 It's complicated false Mutation at a Pst1 restriction site (nucleotide 343). false Eva Meier Carlsen annotation2130035 1 stop range2130035 1 1570 1572 annotation2130034 1 mutation range2130034 1 342 342 annotation2130033 1 start range2130033 1 1 3 annotation2130044 1 Cyp79A2 range2130044 1 1 1572 BBa_K527000_sequence 1 atggctctgttattagcagtttttcttcttcttgcattaactatgaagcgtaaggagaagaagaaaaccatgttaattagccctacgagaaacctctctctccctcccgggccgaaatcttggcctttaatcggaaacctaccggaaatactagggaggaacaaaccggtgttccggtggatacattctctcatgaaagaactcaacaccgatattgcatgtatccgtcttgcgaatactcacgtgatccccgtgacatccccgagaattgcaagagagattctgaagaagcaagactccgttttcgccactagaccgctaacgatgggcacggagtactgtagccgcgggtacttgaccgttgcggtggagccacaaggagagcagtggaagaagatgaggagagtggtggcatctcacgtgacgagcaagaagagcttccaaatgatgctacaaaagagaaccgaagaggctgataacttagtccggtacatcaataaccgtagtgtcaaaaaccgtggtaatgcttttgtggttattgatttaaggcttgcggtacggcaatacagtggaaatgtagctcggaagatgatgtttggtataaggcattttggtaaaggaagtgaagatggatcgggaccagggttggaagagattgaacatgtggaatctttgtttacggttttaacccatctttacgcctttgcattgtcagattatgtcccgtggctaaggttcttggacttggaaggccatgagaaggttgtgagtaacgcaatgagaaatgtaagtaagtataacgacccttttgttgatgaaagactcatgcaatggcgaaatgggaagatgaaagaacctcaagattttcttgacatgtttataatagctaaagacactgacgggaagcctactctgtcggacgaagagatcaaagcacaagtgacggaactaatgttggcgacggttgataatccgtctaacgcggcagagtggggtatggcggagatgattaacgagccgagcatcatgcaaaaagccgtggaagagattgatagggtagttggaaaagaccgtcttgtcattgagtctgatctcccaaatcttaactatgtgaaggcttgtgtgaaagaagcattccggttacaccccgtggcaccgttcaacctccctcacatgtccaccactgatactgtggtagacggttatttcatccccaagggaagccacgtattgattagtcgtatggggattgggagaaatcctagtgtgtgggacaagccgcataagttcgaccctgagagacatttgagcactaacacatgtgtggatctaaacgagtctgatctgaatataatatcgttcagtgcaggacgaagaggttgtatgggtgtggacattgggtcagccatgacgtacatgttactggctcggttgattcaaggattcacgtggttaccagtgcctggtaagaataagattgatatttcagaaagcaagaatgatctttttatggcaaaaccattatacgcggttgccacacctcgtttagctccacatgtgtatccaacctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z