BBa_K527000
1
BBa_K527000
Biosynthesis/Oximes/CYP79A2
2011-09-16T11:00:00Z
2015-05-08T01:12:35Z
Institute of Plant Biology and Biotechnology
Faaculty og LIFE Science
Copenhagen University
Denmark
This BioBrick BBa_K527000 encode the plant enzyme Cytochrome P450 CYP79A2. Cytochromes p450 are defined as heme-thiolate proteins featuring a particular spectral signature at 450 nm, thus the name. Despite the label as cytochromes, these proteins are not involved in electron transfers, but act as monooxygenases in a wide range of reactions such as epoxidation, N-dealkylation, O-dealkylation, S-oxidation and hydroxylation.
CYP79A2 is an N-hydroxylase converting L-phenylalanine into phenylacetaldoxime, the precursor of the cyanogenic glycoside benzylglucosinolate. Oximes as the phenylacetaldoxime is generally toxic to fungi and is frequently used as chemical fungicides. They function by inhibiting mitochondrial oxidases and thereby promote lipid peroxidation and the production of toxic reactive oxygen species.
false
true
_693_
0
8631
9
It's complicated
false
Mutation at a Pst1 restriction site (nucleotide 343).
false
Eva Meier Carlsen
annotation2130035
1
stop
range2130035
1
1570
1572
annotation2130034
1
mutation
range2130034
1
342
342
annotation2130033
1
start
range2130033
1
1
3
annotation2130044
1
Cyp79A2
range2130044
1
1
1572
BBa_K527000_sequence
1
atggctctgttattagcagtttttcttcttcttgcattaactatgaagcgtaaggagaagaagaaaaccatgttaattagccctacgagaaacctctctctccctcccgggccgaaatcttggcctttaatcggaaacctaccggaaatactagggaggaacaaaccggtgttccggtggatacattctctcatgaaagaactcaacaccgatattgcatgtatccgtcttgcgaatactcacgtgatccccgtgacatccccgagaattgcaagagagattctgaagaagcaagactccgttttcgccactagaccgctaacgatgggcacggagtactgtagccgcgggtacttgaccgttgcggtggagccacaaggagagcagtggaagaagatgaggagagtggtggcatctcacgtgacgagcaagaagagcttccaaatgatgctacaaaagagaaccgaagaggctgataacttagtccggtacatcaataaccgtagtgtcaaaaaccgtggtaatgcttttgtggttattgatttaaggcttgcggtacggcaatacagtggaaatgtagctcggaagatgatgtttggtataaggcattttggtaaaggaagtgaagatggatcgggaccagggttggaagagattgaacatgtggaatctttgtttacggttttaacccatctttacgcctttgcattgtcagattatgtcccgtggctaaggttcttggacttggaaggccatgagaaggttgtgagtaacgcaatgagaaatgtaagtaagtataacgacccttttgttgatgaaagactcatgcaatggcgaaatgggaagatgaaagaacctcaagattttcttgacatgtttataatagctaaagacactgacgggaagcctactctgtcggacgaagagatcaaagcacaagtgacggaactaatgttggcgacggttgataatccgtctaacgcggcagagtggggtatggcggagatgattaacgagccgagcatcatgcaaaaagccgtggaagagattgatagggtagttggaaaagaccgtcttgtcattgagtctgatctcccaaatcttaactatgtgaaggcttgtgtgaaagaagcattccggttacaccccgtggcaccgttcaacctccctcacatgtccaccactgatactgtggtagacggttatttcatccccaagggaagccacgtattgattagtcgtatggggattgggagaaatcctagtgtgtgggacaagccgcataagttcgaccctgagagacatttgagcactaacacatgtgtggatctaaacgagtctgatctgaatataatatcgttcagtgcaggacgaagaggttgtatgggtgtggacattgggtcagccatgacgtacatgttactggctcggttgattcaaggattcacgtggttaccagtgcctggtaagaataagattgatatttcagaaagcaagaatgatctttttatggcaaaaccattatacgcggttgccacacctcgtttagctccacatgtgtatccaacctaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z