BBa_K527001
1
BBa_K527001
Biosynthesis/Oximes/CYP79A2/Plug 'n' Play
2011-09-16T11:00:00Z
2015-05-08T01:12:35Z
Institute of Plant Biology and Biotechnology
Faculty of LIFE Science
University of Copenhagen
Denmark
http://2011.igem.org/Team:DTU-Denmark-2
This BioBrick BBa_K527001 encode the plant enzyme Cytochrome P450 CYP79A2. Cytochromes p450 are defined as heme-thiolate proteins featuring a particular spectral signature at 450 nm, thus the name. Despite the label as cytochromes, these proteins are not involved in electron transfers, but act as monooxygenases in a wide range of reactions such as epoxidation, N-dealkylation, O-dealkylation, S-oxidation and hydroxylation.
CYP79A2 is an N-hydroxylase converting L-phenylalanine into phenylacetaldoxime, the precursor of the cyanogenic glycoside benzylglucosinolate. Oximes as the phenylacetaldoxime is generally toxic to fungi and is frequently used as chemical fungicides. They function by inhibiting mitochondrial oxidases and thereby promote lipid peroxidation and the production of toxic reactive oxygen species.
BBa_K527001 codes for the same enzyme as BBa_K527000, but is submitted in an other plasmid due to the different assembly method (Plug 'n' Play) hence the missing prefix and suffix.
false
true
_693_
0
8631
9
It's complicated
false
Mutated at a Pst1 restiction site (nucleotide 342)
No prefix and suffix.
User assembly ends.
false
Eva Meier Carlsen
annotation2131840
1
start
range2131840
1
1
3
annotation2131842
1
stop
range2131842
1
1570
1572
annotation2131854
1
CYP79A2
range2131854
1
1
1572
annotation2131857
1
mutation
range2131857
1
342
342
BBa_K527001_sequence
1
atggctctgttattagcagtttttcttcttcttgcattaactatgaagcgtaaggagaagaagaaaaccatgttaattagccctacgagaaacctctctctccctcccgggccgaaatcttggcctttaatcggaaacctaccggaaatactagggaggaacaaaccggtgttccggtggatacattctctcatgaaagaactcaacaccgatattgcatgtatccgtcttgcgaatactcacgtgatccccgtgacatccccgagaattgcaagagagattctgaagaagcaagactccgttttcgccactagaccgctaacgatgggcacggagtactgtagccgcgggtacttgaccgttgcggtggagccacaaggagagcagtggaagaagatgaggagagtggtggcatctcacgtgacgagcaagaagagcttccaaatgatgctacaaaagagaaccgaagaggctgataacttagtccggtacatcaataaccgtagtgtcaaaaaccgtggtaatgcttttgtggttattgatttaaggcttgcggtacggcaatacagtggaaatgtagctcggaagatgatgtttggtataaggcattttggtaaaggaagtgaagatggatcgggaccagggttggaagagattgaacatgtggaatctttgtttacggttttaacccatctttacgcctttgcattgtcagattatgtcccgtggctaaggttcttggacttggaaggccatgagaaggttgtgagtaacgcaatgagaaatgtaagtaagtataacgacccttttgttgatgaaagactcatgcaatggcgaaatgggaagatgaaagaacctcaagattttcttgacatgtttataatagctaaagacactgacgggaagcctactctgtcggacgaagagatcaaagcacaagtgacggaactaatgttggcgacggttgataatccgtctaacgcggcagagtggggtatggcggagatgattaacgagccgagcatcatgcaaaaagccgtggaagagattgatagggtagttggaaaagaccgtcttgtcattgagtctgatctcccaaatcttaactatgtgaaggcttgtgtgaaagaagcattccggttacaccccgtggcaccgttcaacctccctcacatgtccaccactgatactgtggtagacggttatttcatccccaagggaagccacgtattgattagtcgtatggggattgggagaaatcctagtgtgtgggacaagccgcataagttcgaccctgagagacatttgagcactaacacatgtgtggatctaaacgagtctgatctgaatataatatcgttcagtgcaggacgaagaggttgtatgggtgtggacattgggtcagccatgacgtacatgttactggctcggttgattcaaggattcacgtggttaccagtgcctggtaagaataagattgatatttcagaaagcaagaatgatctttttatggcaaaaccattatacgcggttgccacacctcgtttagctccacatgtgtatccaacctaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z