BBa_K527001 1 BBa_K527001 Biosynthesis/Oximes/CYP79A2/Plug 'n' Play 2011-09-16T11:00:00Z 2015-05-08T01:12:35Z Institute of Plant Biology and Biotechnology Faculty of LIFE Science University of Copenhagen Denmark http://2011.igem.org/Team:DTU-Denmark-2 This BioBrick BBa_K527001 encode the plant enzyme Cytochrome P450 CYP79A2. Cytochromes p450 are defined as heme-thiolate proteins featuring a particular spectral signature at 450 nm, thus the name. Despite the label as cytochromes, these proteins are not involved in electron transfers, but act as monooxygenases in a wide range of reactions such as epoxidation, N-dealkylation, O-dealkylation, S-oxidation and hydroxylation. CYP79A2 is an N-hydroxylase converting L-phenylalanine into phenylacetaldoxime, the precursor of the cyanogenic glycoside benzylglucosinolate. Oximes as the phenylacetaldoxime is generally toxic to fungi and is frequently used as chemical fungicides. They function by inhibiting mitochondrial oxidases and thereby promote lipid peroxidation and the production of toxic reactive oxygen species. BBa_K527001 codes for the same enzyme as BBa_K527000, but is submitted in an other plasmid due to the different assembly method (Plug 'n' Play) hence the missing prefix and suffix. false true _693_ 0 8631 9 It's complicated false Mutated at a Pst1 restiction site (nucleotide 342) No prefix and suffix. User assembly ends. false Eva Meier Carlsen annotation2131857 1 mutation range2131857 1 342 342 annotation2131842 1 stop range2131842 1 1570 1572 annotation2131840 1 start range2131840 1 1 3 annotation2131854 1 CYP79A2 range2131854 1 1 1572 BBa_K527001_sequence 1 atggctctgttattagcagtttttcttcttcttgcattaactatgaagcgtaaggagaagaagaaaaccatgttaattagccctacgagaaacctctctctccctcccgggccgaaatcttggcctttaatcggaaacctaccggaaatactagggaggaacaaaccggtgttccggtggatacattctctcatgaaagaactcaacaccgatattgcatgtatccgtcttgcgaatactcacgtgatccccgtgacatccccgagaattgcaagagagattctgaagaagcaagactccgttttcgccactagaccgctaacgatgggcacggagtactgtagccgcgggtacttgaccgttgcggtggagccacaaggagagcagtggaagaagatgaggagagtggtggcatctcacgtgacgagcaagaagagcttccaaatgatgctacaaaagagaaccgaagaggctgataacttagtccggtacatcaataaccgtagtgtcaaaaaccgtggtaatgcttttgtggttattgatttaaggcttgcggtacggcaatacagtggaaatgtagctcggaagatgatgtttggtataaggcattttggtaaaggaagtgaagatggatcgggaccagggttggaagagattgaacatgtggaatctttgtttacggttttaacccatctttacgcctttgcattgtcagattatgtcccgtggctaaggttcttggacttggaaggccatgagaaggttgtgagtaacgcaatgagaaatgtaagtaagtataacgacccttttgttgatgaaagactcatgcaatggcgaaatgggaagatgaaagaacctcaagattttcttgacatgtttataatagctaaagacactgacgggaagcctactctgtcggacgaagagatcaaagcacaagtgacggaactaatgttggcgacggttgataatccgtctaacgcggcagagtggggtatggcggagatgattaacgagccgagcatcatgcaaaaagccgtggaagagattgatagggtagttggaaaagaccgtcttgtcattgagtctgatctcccaaatcttaactatgtgaaggcttgtgtgaaagaagcattccggttacaccccgtggcaccgttcaacctccctcacatgtccaccactgatactgtggtagacggttatttcatccccaagggaagccacgtattgattagtcgtatggggattgggagaaatcctagtgtgtgggacaagccgcataagttcgaccctgagagacatttgagcactaacacatgtgtggatctaaacgagtctgatctgaatataatatcgttcagtgcaggacgaagaggttgtatgggtgtggacattgggtcagccatgacgtacatgttactggctcggttgattcaaggattcacgtggttaccagtgcctggtaagaataagattgatatttcagaaagcaagaatgatctttttatggcaaaaccattatacgcggttgccacacctcgtttagctccacatgtgtatccaacctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z