BBa_K527002 1 BBa_K527002 Biosynthesis/Oximes/CYP79B1/Plug 'n' Play 2011-09-16T11:00:00Z 2015-05-08T01:12:35Z Institute of Plant Biology and Biotechnology Faculty of LIFE Science University of Copenhagen Denmark http://2011.igem.org/Team:DTU-Denmark-2 This BioBrick BBa_K527002 encode the plant enzyme Cytochrome P450 CYP79B1. Cytochromes p450 are defined as heme-thiolate proteins featuring a particular spectral signature at 450 nm, thus the name. Despite the label as cytochromes, these proteins are not involved in electron transfers, but act as monooxygenases in a wide range of reactions such as epoxidation, N-dealkylation, O-dealkylation, S-oxidation and hydroxylation. CYP79B1 is an N-hydroxylase converting L-Tryptophan into indole 3-acetaldoxime, the precursor of a cyanogenic glycoside . Oximes as the indole 3-acetaldoxime is generally toxic to fungi and is frequently used as a chemical fungicide. They function by inhibiting mitochondrial oxidases and thereby promote lipid peroxidation and the production of toxic reactive oxygen species. BBa_K527002 is submitted in the plasmid BBa_K678023 due to the different assembly method (Plug 'n' Play) hence the missing prefix and suffix. false false _693_ 0 8631 9 It's complicated false Mutated at a Pst1 restiction site (nucleotide 342) No prefix and suffix. User assembly ends. false Eva Meier Carlsen annotation2131869 1 mutation range2131869 1 852 852 annotation2131870 1 CYP79B1 range2131870 1 1 1560 annotation2131865 1 start range2131865 1 1 3 annotation2131868 1 stop range2131868 1 1558 1560 BBa_K527002_sequence 1 atgtatttacttacaacgcttcaagcctttgtggctataaccttggtgatgcttctcaagaaagtattggtcaacgatactaataaaaagaaattgtctctcccgccgggtcccactggatggccgatcatcggcatggttccaacgatgcttaagagccgtcccgttttccggtggctccatagcatcatgaagcagctaaacaccgagatagcctgcgtgaggctaggaagcactcacgtgataaccgtcacatgccctaagatagcacgtgaggtactgaagcaacaagacgctctcttcgcctcaagacctatgacttacgcacagaatgtcctctctaacggatacaaaacctgcgtgatcactccgttcggtgaacaattcaagaaaatgaggaaagtggtgatgactgaactcgtttgtcccgcgagacacaggtggctacatcagaagagagctgaagagaacgatcatttaaccgcttgggtatacaacatggttaacaactctgactcggtcgatttccggttcgtcacgaggcattactgtggaaatgctatcaagaagctcatgttcgggacaagaacgttctctcaaaacactgcaccgaacggtggaccaaccgcagaggatatcgagcatatggaagctatgttcgaagcattagggtttacgttctctttttgcatctctgattatctaccgattctcacgggacttgatcttaacggccacgagaagatcatgagagattcaagtgctattatggacaagtatcacgatccgatcatcgatgcaaggatcaagatgtggagagaaggaaagaaaactcaaatcgaggattttcttgacattttcatttccatcaaggatgaagaaggaaacccattgcttaccgcagatgaaatcaaaccgaccattaaggagcttgtaatggcggcgccagacaatccatcaaacgccgtagaatgggccatggcggagatggtgaacaaaccggagattctccgcaaagcaatggaagaaatcgacagagttgtcgggaaagaaagacttgtccaagaatccgacatcccaaaactaaactacgtcaaagctattctccgtgaagcattccgtctacatcccgtcgccgcctttaacctcccacacgtggcactttccgacgcaaccgtcgccggatatcacatccctaaaggaagtcaagtccttcttagccgatatgggctgggccgtaacccgaaagtttgggccgacccactgagctttaaaccggagagacatctcaacgaatgctcggaagttactttgacggagaacgatctccggtttatatcgtttagtaccgggaaaagaggttgtgctgctccggctttaggtacggcgttgaccacgatgttgctcgcgagacttctacaaggtttcacttggaagctgccggagaatgagacacgtgttgagctgatggagtctagtcatgatatgtttttggctaaaccattggttatggtcggtgagttgagactaccggaacatctttaccctacagtgaagtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z