BBa_K527002
1
BBa_K527002
Biosynthesis/Oximes/CYP79B1/Plug 'n' Play
2011-09-16T11:00:00Z
2015-05-08T01:12:35Z
Institute of Plant Biology and Biotechnology
Faculty of LIFE Science
University of Copenhagen
Denmark
http://2011.igem.org/Team:DTU-Denmark-2
This BioBrick BBa_K527002 encode the plant enzyme Cytochrome P450 CYP79B1. Cytochromes p450 are defined as heme-thiolate proteins featuring a particular spectral signature at 450 nm, thus the name. Despite the label as cytochromes, these proteins are not involved in electron transfers, but act as monooxygenases in a wide range of reactions such as epoxidation, N-dealkylation, O-dealkylation, S-oxidation and hydroxylation.
CYP79B1 is an N-hydroxylase converting L-Tryptophan into indole 3-acetaldoxime, the precursor of a cyanogenic glycoside . Oximes as the indole 3-acetaldoxime is generally toxic to fungi and is frequently used as a chemical fungicide. They function by inhibiting mitochondrial oxidases and thereby promote lipid peroxidation and the production of toxic reactive oxygen species.
BBa_K527002 is submitted in the plasmid BBa_K678023 due to the different assembly method (Plug 'n' Play) hence the missing prefix and suffix.
false
false
_693_
0
8631
9
It's complicated
false
Mutated at a Pst1 restiction site (nucleotide 342)
No prefix and suffix.
User assembly ends.
false
Eva Meier Carlsen
annotation2131865
1
start
range2131865
1
1
3
annotation2131869
1
mutation
range2131869
1
852
852
annotation2131868
1
stop
range2131868
1
1558
1560
annotation2131870
1
CYP79B1
range2131870
1
1
1560
BBa_K527002_sequence
1
atgtatttacttacaacgcttcaagcctttgtggctataaccttggtgatgcttctcaagaaagtattggtcaacgatactaataaaaagaaattgtctctcccgccgggtcccactggatggccgatcatcggcatggttccaacgatgcttaagagccgtcccgttttccggtggctccatagcatcatgaagcagctaaacaccgagatagcctgcgtgaggctaggaagcactcacgtgataaccgtcacatgccctaagatagcacgtgaggtactgaagcaacaagacgctctcttcgcctcaagacctatgacttacgcacagaatgtcctctctaacggatacaaaacctgcgtgatcactccgttcggtgaacaattcaagaaaatgaggaaagtggtgatgactgaactcgtttgtcccgcgagacacaggtggctacatcagaagagagctgaagagaacgatcatttaaccgcttgggtatacaacatggttaacaactctgactcggtcgatttccggttcgtcacgaggcattactgtggaaatgctatcaagaagctcatgttcgggacaagaacgttctctcaaaacactgcaccgaacggtggaccaaccgcagaggatatcgagcatatggaagctatgttcgaagcattagggtttacgttctctttttgcatctctgattatctaccgattctcacgggacttgatcttaacggccacgagaagatcatgagagattcaagtgctattatggacaagtatcacgatccgatcatcgatgcaaggatcaagatgtggagagaaggaaagaaaactcaaatcgaggattttcttgacattttcatttccatcaaggatgaagaaggaaacccattgcttaccgcagatgaaatcaaaccgaccattaaggagcttgtaatggcggcgccagacaatccatcaaacgccgtagaatgggccatggcggagatggtgaacaaaccggagattctccgcaaagcaatggaagaaatcgacagagttgtcgggaaagaaagacttgtccaagaatccgacatcccaaaactaaactacgtcaaagctattctccgtgaagcattccgtctacatcccgtcgccgcctttaacctcccacacgtggcactttccgacgcaaccgtcgccggatatcacatccctaaaggaagtcaagtccttcttagccgatatgggctgggccgtaacccgaaagtttgggccgacccactgagctttaaaccggagagacatctcaacgaatgctcggaagttactttgacggagaacgatctccggtttatatcgtttagtaccgggaaaagaggttgtgctgctccggctttaggtacggcgttgaccacgatgttgctcgcgagacttctacaaggtttcacttggaagctgccggagaatgagacacgtgttgagctgatggagtctagtcatgatatgtttttggctaaaccattggttatggtcggtgagttgagactaccggaacatctttaccctacagtgaagtaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z