LOCUS BBa_K530036 5690 bp DNA linear 22-Dec-2024 DEFINITION pRSBB423 HIS3 2Micron Episomal Yeast Shuttle Vector ACCESSION BBa_K530036 VERSION BBa_K530036.1 FEATURES Location/Qualifiers ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt 241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta 301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat 361 tttttttttt cccctagcgg atgactcttt ttttttctta gcgattggca ttatcacata 421 atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc 481 aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa 541 atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact 601 cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga 661 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt 721 ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca 781 ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctactg gcgcgtggag 841 taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag 901 atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag 961 atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggca agcagaatta 1021 ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca 1081 aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct 1141 ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat 1201 atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat 1261 actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt 1321 ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc tatgcggtgt 1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata 1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt 1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc 1921 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc 1981 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg 2041 agcgcgcgta atacgactca ctatagggcg aattgcagct tttgttccct ttagtgaggg 2101 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg 2161 ctcacaattc cacacaacat aggagccgga agcataaagt gtaaagcctg gggtgcctaa 2221 tgagtgaggt aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 2281 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt 2341 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 2401 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 2461 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 2521 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 2581 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 2641 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 2701 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 2761 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 2821 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 2881 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 2941 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 3001 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 3061 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 3121 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 3181 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 3241 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 3301 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 3361 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 3421 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 3481 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 3541 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 3601 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 3661 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 3721 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 3781 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 3841 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 3901 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 3961 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 4021 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 4081 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 4141 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 4201 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 4261 ccccgaaaag tgccacctga acgaagcatc tgtgcttcat tttgtagaac aaaaatgcaa 4321 cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg 4381 caacgcgaaa gcgctatttt accaacgaag aatctgtgct tcatttttgt aaaacaaaaa 4441 tgcaacgcga gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag 4501 aaatgcaacg cgagagcgct attttaccaa caaagaatct atacttcttt tttgttctac 4561 aaaaatgcat cccgagagcg ctatttttct aacaaagcat cttagattac tttttttctc 4621 ctttgtgcgc tctataatgc agtctcttga taactttttg cactgtaggt ccgttaaggt 4681 tagaagaagg ctactttggt gtctattttc tcttccataa aaaaagcctg actccacttc 4741 ccgcgtttac tgattactag cgaagctgcg ggtgcatttt ttcaagataa aggcatcccc 4801 gattatattc tataccgatg tggattgcgc atactttgtg aacagaaagt gatagcgttg 4861 atgattcttc attggtcaga aaattatgaa cggtttcttc tattttgtct ctatatacta 4921 cgtataggaa atgtttacat tttcgtattg ttttcgattc actctatgaa tagttcttac 4981 tacaattttt ttgtctaaag agtaatacta gagataaaca taaaaaatgt agaggtcgag 5041 tttagatgca agttcaagga gcgaaaggtg gatgggtagg ttatataggg atatagcaca 5101 gagatatata gcaaagagat acttttgagc aatgtttgtg gaagcggtat tcgcaatatt 5161 ttagtagctc gttacagtcc ggtgcgtttt tggttttttg aaagtgcgtc ttcagagcgc 5221 ttttggtttt caaaagcgct ctgaagttcc tatactttct agagaatagg aacttcggaa 5281 taggaacttc aaagcgtttc cgaaaacgag cgcttccgaa aatgcaacgc gagctgcgca 5341 catacagctc actgttcacg tcgcacctat atctgcgtgt tgcctgtata tatatataca 5401 tgagaagaac ggcatagtgc gtgtttatgc ttaaatgcgt acttatatgc gtctatttat 5461 gtaggatgaa aggtagtcta gtacctcctg tgatattatc ccattccatg cggggtatcg 5521 tatgcttcct tcagcactac cctttagctg ttctatatgc tgccactcct caattggatt 5581 agtctcatcc ttcaatgcta tcatttcctt tgatattgga tcatactaag aaaccattat 5641 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc //