LOCUS BBa_K530037 5508 bp DNA linear 22-Dec-2024 DEFINITION pRSBB424 TRP1 2Micron Episomal Yeast Shuttle Vector ACCESSION BBa_K530037 VERSION BBa_K530037.1 FEATURES Location/Qualifiers ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accataaacg acattactat atatataata taggaagcat ttaatagaca gcatcgtaat 241 atatgtgtac tttgcagtta tgacgccaga tggcagtagt ggaagatatt ctttattgaa 301 aaatagcttg tcaccttacg tacaatcttg atccggagct tttctttttt tgccgattaa 361 gaattaattc ggtcgaaaaa agaaaaggag agggccaaga gggagggcat tggtgactat 421 tgagcacgtg agtatacgtg attaagcaca caaaggcagc ttggagtatg tctgttatta 481 atttcacagg tagttctggt ccattggtga aagtttgcgg cttgcagagc acagaggccg 541 cagaatgtgc tctagattcc gatgctgact tgctgggtat tatatgtgtg cccaatagaa 601 agagaacaat tgacccggtt attgcaagga aaatttcaag tcttgtaaaa gcatataaaa 661 atagttcagg cactccgaaa tacttggttg gcgtgtttcg taatcaacct aaggaggatg 721 ttttggctct ggtcaatgat tacggcattg atatcgtcca actgcatgga gatgagtcgt 781 ggcaagaata ccaagagttc ctcggtttgc cagttattaa aagactcgta tttccaaaag 841 actgcaacat actactcagt gcagcttcac agaaacctca ttcgtttatt cccttgtttg 901 attcagaagc aggtgggaca ggtgaacttt tggattggaa ctcgatttct gactgggttg 961 gaaggcaaga gagccccgaa agcttacatt ttatgttagc tggtggactg acgccagaaa 1021 atgttggtga tgcgcttaga ttaaatggcg ttattggtgt tgatgtaagc ggaggtgtgg 1081 agacaaatgg tgtaaaagac tctaacaaaa tagcaaattt cgtcaaaaat gctaagaaat 1141 aggttattac tgagtagtat ttatttaagt attgtttgtg cacttgccta tgcggtgtga 1201 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg aaattgtaaa cgttaatatt 1261 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa 1321 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca 1381 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc 1441 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg 1501 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg 1561 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg 1621 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg 1681 ccgctacagg gcgcgtcgcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga 1741 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga 1801 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgag 1861 cgcgcgtaat acgactcact atagggcgaa ttgcagcttt tgttcccttt agtgagggtt 1921 aattgcgcgc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct 1981 cacaattcca cacaacatag gagccggaag cataaagtgt aaagcctggg gtgcctaatg 2041 agtgaggtaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct 2101 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg 2161 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 2221 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 2281 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 2341 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 2401 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 2461 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 2521 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 2581 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 2641 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 2701 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 2761 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 2821 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 2881 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 2941 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 3001 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 3061 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 3121 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 3181 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 3241 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 3301 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 3361 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 3421 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 3481 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 3541 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 3601 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 3661 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 3721 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 3781 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 3841 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 3901 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 3961 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 4021 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 4081 ccgaaaagtg ccacctgaac gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg 4141 cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca 4201 acgcgaaagc gctattttac caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg 4261 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa 4321 atgcaacgcg agagcgctat tttaccaaca aagaatctat acttcttttt tgttctacaa 4381 aaatgcatcc cgagagcgct atttttctaa caaagcatct tagattactt tttttctcct 4441 ttgtgcgctc tataatgcag tctcttgata actttttgca ctgtaggtcc gttaaggtta 4501 gaagaaggct actttggtgt ctattttctc ttccataaaa aaagcctgac tccacttccc 4561 gcgtttactg attactagcg aagctgcggg tgcatttttt caagataaag gcatccccga 4621 ttatattcta taccgatgtg gattgcgcat actttgtgaa cagaaagtga tagcgttgat 4681 gattcttcat tggtcagaaa attatgaacg gtttcttcta ttttgtctct atatactacg 4741 tataggaaat gtttacattt tcgtattgtt ttcgattcac tctatgaata gttcttacta 4801 caattttttt gtctaaagag taatactaga gataaacata aaaaatgtag aggtcgagtt 4861 tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt atatagggat atagcacaga 4921 gatatatagc aaagagatac ttttgagcaa tgtttgtgga agcggtattc gcaatatttt 4981 agtagctcgt tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt cagagcgctt 5041 ttggttttca aaagcgctct gaagttccta tactttctag agaataggaa cttcggaata 5101 ggaacttcaa agcgtttccg aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca 5161 tacagctcac tgttcacgtc gcacctatat ctgcgtgttg cctgtatata tatatacatg 5221 agaagaacgg catagtgcgt gtttatgctt aaatgcgtac ttatatgcgt ctatttatgt 5281 aggatgaaag gtagtctagt acctcctgtg atattatccc attccatgcg gggtatcgta 5341 tgcttccttc agcactaccc tttagctgtt ctatatgctg ccactcctca attggattag 5401 tctcatcctt caatgctatc atttcctttg atattggatc atactaagaa accattatta 5461 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc //