LOCUS BBa_K530038 6741 bp DNA linear 25-Nov-2024 DEFINITION pRSBB425 LEU2 2Micron Episomal Yeast Shuttle Vector ACCESSION BBa_K530038 VERSION BBa_K530038.1 FEATURES Location/Qualifiers ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc 241 accattatgg gaaatggttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca 301 ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatcaaat 361 taggaatcgt agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc 421 ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc 481 aatttgctta cctgtattcc tttactatcc tcctttttct ccttcttgat aaatgtatgt 541 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg 601 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct 661 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg 721 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct 781 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac 841 aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga accgtggcat 901 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc 961 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg 1021 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca 1081 gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat ggtttcctcc 1141 acagtttttc tccataatct tgaagaggcc aaaacattag ctttatccaa ggaccaaata 1201 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact 1261 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc 1321 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca 1381 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt 1441 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca 1501 ctaccggtac cccatttagg accacccaca gcacctaaca aaacggcatc aaccttcttg 1561 gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc agcaccacca 1621 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga 1681 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc 1741 ttcttagggg cagacatagg ggcagacatt agaatggtat atccttgaaa tatatatata 1801 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat 1861 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat 1921 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct cacctttcct 1981 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca 2041 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat 2101 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga 2161 gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt agaccgctcg 2221 gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa tataacgttt 2281 ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat gtggattttg 2341 atgtaattgt tgggattcca tttttaataa ggcaataata ttaggtatgt ggatatacta 2401 gaagttctcc tcgaccgtcg atatgcggtg tgaaataccg cacagatgcg taaggagaaa 2461 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt 2521 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa 2581 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag 2641 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt 2701 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac 2761 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 2821 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 2881 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg 2941 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 3001 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc 3061 cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc 3121 gaattgcagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc 3181 atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca taggagccgg 3241 aagcataaag tgtaaagcct ggggtgccta atgagtgagg taactcacat taattgcgtt 3301 gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg 3361 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 3421 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 3481 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 3541 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 3601 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 3661 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 3721 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc 3781 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 3841 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 3901 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 3961 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 4021 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 4081 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 4141 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 4201 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 4261 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 4321 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 4381 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 4441 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 4501 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 4561 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 4621 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 4681 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 4741 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 4801 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 4861 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 4921 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 4981 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 5041 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 5101 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 5161 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 5221 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 5281 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg aacgaagcat 5341 ctgtgcttca ttttgtagaa caaaaatgca acgcgagagc gctaattttt caaacaaaga 5401 atctgagctg catttttaca gaacagaaat gcaacgcgaa agcgctattt taccaacgaa 5461 gaatctgtgc ttcatttttg taaaacaaaa atgcaacgcg agagcgctaa tttttcaaac 5521 aaagaatctg agctgcattt ttacagaaca gaaatgcaac gcgagagcgc tattttacca 5581 acaaagaatc tatacttctt ttttgttcta caaaaatgca tcccgagagc gctatttttc 5641 taacaaagca tcttagatta ctttttttct cctttgtgcg ctctataatg cagtctcttg 5701 ataacttttt gcactgtagg tccgttaagg ttagaagaag gctactttgg tgtctatttt 5761 ctcttccata aaaaaagcct gactccactt cccgcgttta ctgattacta gcgaagctgc 5821 gggtgcattt tttcaagata aaggcatccc cgattatatt ctataccgat gtggattgcg 5881 catactttgt gaacagaaag tgatagcgtt gatgattctt cattggtcag aaaattatga 5941 acggtttctt ctattttgtc tctatatact acgtatagga aatgtttaca ttttcgtatt 6001 gttttcgatt cactctatga atagttctta ctacaatttt tttgtctaaa gagtaatact 6061 agagataaac ataaaaaatg tagaggtcga gtttagatgc aagttcaagg agcgaaaggt 6121 ggatgggtag gttatatagg gatatagcac agagatatat agcaaagaga tacttttgag 6181 caatgtttgt ggaagcggta ttcgcaatat tttagtagct cgttacagtc cggtgcgttt 6241 ttggtttttt gaaagtgcgt cttcagagcg cttttggttt tcaaaagcgc tctgaagttc 6301 ctatactttc tagagaatag gaacttcgga ataggaactt caaagcgttt ccgaaaacga 6361 gcgcttccga aaatgcaacg cgagctgcgc acatacagct cactgttcac gtcgcaccta 6421 tatctgcgtg ttgcctgtat atatatatac atgagaagaa cggcatagtg cgtgtttatg 6481 cttaaatgcg tacttatatg cgtctattta tgtaggatga aaggtagtct agtacctcct 6541 gtgatattat cccattccat gcggggtatc gtatgcttcc ttcagcacta ccctttagct 6601 gttctatatg ctgccactcc tcaattggat tagtctcatc cttcaatgct atcatttcct 6661 ttgatattgg atcatactaa gaaaccatta ttatcatgac attaacctat aaaaataggc 6721 gtatcacgag gccctttcgt c //