BBa_K531007 1 BBa_K531007 P_rsaA constitutive promoter, <i>esp</i> optimized for <i>Caulobacter</i>, and <i>rsaA</i> 2011-09-20T11:00:00Z 2015-05-08T01:12:36Z P_rsaA and <i>rsaA</i> C-terminal secretion tag both come from the genome of <i>Caulobacter crescentus</i>; <i>esp</i> optimized for <i>Caulobacter crescentus</i> is a synthetically codon optimized copy of <i>esp</i> as it is found in <i>Staphylococcus epidermidis</i>. P_rsaA constitutive promoter from <i>Caulobacter crescentus</i>, <i>esp</i>, a suspected serine protease from <i>Staphylococcus epidermidis</i>, optimized for expression in <i>Caulobacter crescentus</i>, and the C-terminal secretion tag from <i>rsaA</i>. false false _697_ 0 8840 9 It's complicated true Stop codon naturally occurring at the end of <i>esp</i> removed to ensure smooth expression of the fusion protein. false Alexander Aaring component2136609 1 BBa_K531001 component2136608 1 BBa_K531006 annotation2136609 1 BBa_K531001 range2136609 1 969 1328 annotation2136608 1 BBa_K531006 range2136608 1 96 960 BBa_K531001 1 BBa_K531001 <i>C. crescentus</i> S-layer RsaA C-term 2011-07-03T11:00:00Z 2015-05-08T01:12:36Z This comes from genomic ''Caulobacter'' CB15N. This is a secretion tag for secretion via a Type I secretion pathway in ''Caulobacter crescentus''. It should be appended to the C-terminal end of another protein to act as a secretion tag. false false _697_ 0 8840 9 In stock true Using the Registry's suggested primer combinations results in the formation of an in sequence stop codon between two coding regions. A one nucleotide change was necessary in both the prefix of this coding sequence and the suffix of the coding region to be added in front of this gene to ensure that their is no stop codon in sequence. false Alexander Aaring BBa_K531006 1 BBa_K531006 Optimized esp gene for <i>C. crescentus</i> 2011-07-25T11:00:00Z 2015-05-08T01:12:36Z Wild type esp sequence from Zhang,Y.Q., Ren,S.X., Li,H.L., Wang,Y.X., Fu,G., Yang,J., Qin,Z.Q., Miao,Y.G., Wang,W.Y., Chen,R.S., Shen,Y., Chen,Z., Yuan,Z.H., Zhao,G.P., Qu,D., Danchin,A. and Wen,Y.M. Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228). Mol. Microbiol. 49 (6), 1577-1593 (2003). Optimized sequence is based on the wild type sequence. The wild Type esp is secreted by S. epidermidis, a bacteria that has low G/C content in its genome. It is then optimized to give a high G/C content without changing any amino acids sequence. false false _697_ 0 8860 9 It's complicated false DNA codons are optimized for <i>C. crescentus</i> expression. false Qimeng Gao BBa_K531007_sequence 1 ttgtcgacgtatgacgtttgctctatagccatcgctgctcccatgcgcgccactcggtcgcagggggtgtgggattttttttgggagtactagagtgaagaagcgcttcctgtcgatctgcaccatgaccatcgccgccctggccacgacgaccatggtgaacacgagctatgcgaagaccgacaccgagtcccacaaccactcgtcgctgggtaccgaaaacaagaatgtcctggacatcaacagcagctcccacaacatcaagccgagccagaacaagagctatccgagcgtgatcctgccgaataacaaccgccaccagatcttcaacacgacccaagggcactatgacgccgtgtcgttcatctacatcccgatcgacggcggctacatcccgatcgacggcggctacatgagcggctccggcgtcgtcgtcggcgaaacgagatcctcacgaataagcacgtcgtgaacggcgcgaagggcaacccccggaatatctccgtccatccctcggccaagaacgagaacgattatcccaatggtaagttcgtgggccaagaaatcatcccgtaccccggtaactcggacctggccatcctgcgcgtgagccccaacgagcacaatcaacacatcggccaggtggtcaagcccgcgacgatcagcagcaacacggacacccgcatcaacgagaacatcaccgtcaccggctaccccggcgacaagcccctggcgaccatgtgggagagcgtcggcaaggtggtctatatcggcggcgaggagctgcgctatgacctcagcacggtgggcggcaacagcggctcgcccgtcttcaacggcaagaaccaggtgatcggcatccactatggtggtgtcgacaacaagtataactcgagcgtctacatcaatgacttcgtgcagcaattcctgcgcaacaacatcccggacatcaacatccagtactagaggatatcaacgctatcggcacctcgaccgctttcgtgacgatcaccgacgccgctgtcggcgacaagctcgacctcgtcggcatctcgacgaacggcgctatcgctgacggcgccttcggcgctgcggtcaccctgggcgctgctgcgaccctggctcagtacctggacgctgctgctgccggcgacggcagcggcacctcggttgccaagtggttccagttcggcggcgacacctatgtcgtcgttgacagctcggctggcgcgaccttcgtcagcggcgctgacgcggtgatcaagctgaccggtctggtcacgctgaccacctcggccttcgccaccgaagtcctgacgctcgcctaa BBa_K531006_sequence 1 tgaagaagcgcttcctgtcgatctgcaccatgaccatcgccgccctggccacgacgaccatggtgaacacgagctatgcgaagaccgacaccgagtcccacaaccactcgtcgctgggtaccgaaaacaagaatgtcctggacatcaacagcagctcccacaacatcaagccgagccagaacaagagctatccgagcgtgatcctgccgaataacaaccgccaccagatcttcaacacgacccaagggcactatgacgccgtgtcgttcatctacatcccgatcgacggcggctacatcccgatcgacggcggctacatgagcggctccggcgtcgtcgtcggcgaaacgagatcctcacgaataagcacgtcgtgaacggcgcgaagggcaacccccggaatatctccgtccatccctcggccaagaacgagaacgattatcccaatggtaagttcgtgggccaagaaatcatcccgtaccccggtaactcggacctggccatcctgcgcgtgagccccaacgagcacaatcaacacatcggccaggtggtcaagcccgcgacgatcagcagcaacacggacacccgcatcaacgagaacatcaccgtcaccggctaccccggcgacaagcccctggcgaccatgtgggagagcgtcggcaaggtggtctatatcggcggcgaggagctgcgctatgacctcagcacggtgggcggcaacagcggctcgcccgtcttcaacggcaagaaccaggtgatcggcatccactatggtggtgtcgacaacaagtataactcgagcgtctacatcaatgacttcgtgcagcaattcctgcgcaacaacatcccggacatcaacatccag BBa_K531001_sequence 1 gatatcaacgctatcggcacctcgaccgctttcgtgacgatcaccgacgccgctgtcggcgacaagctcgacctcgtcggcatctcgacgaacggcgctatcgctgacggcgccttcggcgctgcggtcaccctgggcgctgctgcgaccctggctcagtacctggacgctgctgctgccggcgacggcagcggcacctcggttgccaagtggttccagttcggcggcgacacctatgtcgtcgttgacagctcggctggcgcgaccttcgtcagcggcgctgacgcggtgatcaagctgaccggtctggtcacgctgaccacctcggccttcgccaccgaagtcctgacgctcgcctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z