BBa_K531004 1 BBa_K531004 dspB from Actinobacillus actinomycetemcomitansis optimized for Caulobacter crescentus 2011-07-10T11:00:00Z 2015-05-08T01:12:36Z Kaplan, J., Ragunath, C., Ramasubbu, N., and Fine, D. Detachment of Actinobacillus actinomycetemcomitans Biofilm Cells by an Endogenous beta-Hexosaminidase Activity. Journal of Bacteriology. 2003 August; 185(16): 4693-4698. DspB comes from the bacteria Actinobacillus actinomycetemcomitansis. It is most likely an N-acetylglucosaminidase that causes the detachment of cells from biofilm colonies through the 1&#8594; 4 glycosidic bond of &#946;-substituted N-acetylglucosamine. DspB is homologous to family 20 glycosyl hydrolases which cleave terminal monosaccharide residues. false false _697_ 0 9994 9 Not in stock false The WT gene has a low GC content ~40%. Caulobacter crescentus is GC rich and prefers a high GC content. Therefore, we optimized the GC content by comparing the codons in the wild type dspB gene to their corresponding amino acid codons that are most expressed in Caulobacter. We also eliminated the stop codon at the end of dspB because we wanted to add another gene to it that also needed to be transcribed. false Nora Kostow BBa_K531001 1 BBa_K531001 <i>C. crescentus</i> S-layer RsaA C-term 2011-07-03T11:00:00Z 2015-05-08T01:12:36Z This comes from genomic ''Caulobacter'' CB15N. This is a secretion tag for secretion via a Type I secretion pathway in ''Caulobacter crescentus''. It should be appended to the C-terminal end of another protein to act as a secretion tag. false false _697_ 0 8840 9 In stock true Using the Registry's suggested primer combinations results in the formation of an in sequence stop codon between two coding regions. A one nucleotide change was necessary in both the prefix of this coding sequence and the suffix of the coding region to be added in front of this gene to ensure that their is no stop codon in sequence. false Alexander Aaring BBa_K531009 1 BBa_K531009 P_rsaA + <i>Caulobacter</i> optimized <i>dspB</i> + <i>rsaA</i> C-term 2011-09-22T11:00:00Z 2015-05-08T01:12:36Z P<sub>rsaA</i> and <i>rsaA</i> C-terminal secretion tag both come from the genome of <i>Caulobacter</i> lab strain CB15N. IDT synthesized <i>dspB</i> so as to codon optimize it for expression in <I>Caulobacter</i>. Constitutive promoter P<sub>rsaA</i> with <i>dspB</i> codon optimized for <i>Caulobacter crescentus</i> and the C-terminal secretion tag of <i>Caulobacter's</i> S-layer associated protein, RsaA. false false _697_ 0 8840 9 It's complicated false Stop codon at the end of <i>dspB</i> removed to ensure expression of the <i>rsaA</i> C-terminal. false Alexander Aaring component2156661 1 BBa_K531004 component2156662 1 BBa_K531001 annotation2156661 1 BBa_K531004 range2156661 1 96 1180 annotation2156662 1 BBa_K531001 range2156662 1 1189 1548 BBa_K531009_sequence 1 ttgtcgacgtatgacgtttgctctatagccatcgctgctcccatgcgcgccactcggtcgcagggggtgtgggattttttttgggagtactagagtgaactgctgcgtgaagggcaactccatctatccgcagaagacctccaccaagcagaccggcctcatgctggatatcgcccgccacttctactcgccggaagtgatcaagtcgttcatcgacaccatctcgctgagcggcggcaacttcctgcacctgcacttctccgaccatgaaaactatgcgatcgagtcgcacctcctcaaccagcgcgcggaaaacgccgtccagggcaaggacggcatctacatcaacccgtacaccggcaagccgttcctgagctatcgccagctggacgacatcaaggcgtatgcgaaggccaaggggatcgagctgatccccgagctggacagccccaaccacatgaccgccatcttcaagctggtccagaaggaccgcggtgtcaagtacctccagggcctcaagagccgccaggtcgacgacgagatcgacatcaccaacgcggactcgatcacgttcatgcagtccctgatgtcggaggtcatcgacatcttcggggacacgtcgcagcatttccatatcggtggcgacgagttcggctatagcgtcgagagcaaccatgagttcatcacctacgccaacaagctgagctacttcctggaaaagaagggcctgaagacccgcatgtggaacgatggcctgatcaagaatacctttgaacagatcaatcccaacatcgagatcacgtactggagctatgatggcgacacgcaagacaagaacgaggcggccgaacgccgcgacatgcgggtctcgctgccggagctgctggccaagggtttcaccgtgctcaattacaattcctattacctgtatatcgtcccgaaggcctcgccgaccttcagccaggacgcggcctttgccgccaaggatgtcatcaagaactgggatctgggggtctgggacggccgcaataccaagaaccgggtccagaacacccatgagatcgccggcgccgccctctcgatctggggcgaggacgccaaggccctgaaggacgaaacgatccagaagaacaccaagagcctgctcgaagccgtgatccacaagaccaatggcgatgagtactagaggatatcaacgctatcggcacctcgaccgctttcgtgacgatcaccgacgccgctgtcggcgacaagctcgacctcgtcggcatctcgacgaacggcgctatcgctgacggcgccttcggcgctgcggtcaccctgggcgctgctgcgaccctggctcagtacctggacgctgctgctgccggcgacggcagcggcacctcggttgccaagtggttccagttcggcggcgacacctatgtcgtcgttgacagctcggctggcgcgaccttcgtcagcggcgctgacgcggtgatcaagctgaccggtctggtcacgctgaccacctcggccttcgccaccgaagtcctgacgctcgcctaa BBa_K531001_sequence 1 gatatcaacgctatcggcacctcgaccgctttcgtgacgatcaccgacgccgctgtcggcgacaagctcgacctcgtcggcatctcgacgaacggcgctatcgctgacggcgccttcggcgctgcggtcaccctgggcgctgctgcgaccctggctcagtacctggacgctgctgctgccggcgacggcagcggcacctcggttgccaagtggttccagttcggcggcgacacctatgtcgtcgttgacagctcggctggcgcgaccttcgtcagcggcgctgacgcggtgatcaagctgaccggtctggtcacgctgaccacctcggccttcgccaccgaagtcctgacgctcgcctaa BBa_K531004_sequence 1 tgaactgctgcgtgaagggcaactccatctatccgcagaagacctccaccaagcagaccggcctcatgctggatatcgcccgccacttctactcgccggaagtgatcaagtcgttcatcgacaccatctcgctgagcggcggcaacttcctgcacctgcacttctccgaccatgaaaactatgcgatcgagtcgcacctcctcaaccagcgcgcggaaaacgccgtccagggcaaggacggcatctacatcaacccgtacaccggcaagccgttcctgagctatcgccagctggacgacatcaaggcgtatgcgaaggccaaggggatcgagctgatccccgagctggacagccccaaccacatgaccgccatcttcaagctggtccagaaggaccgcggtgtcaagtacctccagggcctcaagagccgccaggtcgacgacgagatcgacatcaccaacgcggactcgatcacgttcatgcagtccctgatgtcggaggtcatcgacatcttcggggacacgtcgcagcatttccatatcggtggcgacgagttcggctatagcgtcgagagcaaccatgagttcatcacctacgccaacaagctgagctacttcctggaaaagaagggcctgaagacccgcatgtggaacgatggcctgatcaagaatacctttgaacagatcaatcccaacatcgagatcacgtactggagctatgatggcgacacgcaagacaagaacgaggcggccgaacgccgcgacatgcgggtctcgctgccggagctgctggccaagggtttcaccgtgctcaattacaattcctattacctgtatatcgtcccgaaggcctcgccgaccttcagccaggacgcggcctttgccgccaaggatgtcatcaagaactgggatctgggggtctgggacggccgcaataccaagaaccgggtccagaacacccatgagatcgccggcgccgccctctcgatctggggcgaggacgccaaggccctgaaggacgaaacgatccagaagaacaccaagagcctgctcgaagccgtgatccacaagaccaatggcgatgag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z