BBa_K081005
1
BBa_K081005
constitutive promoter family member and RBS
2008-10-17T11:00:00Z
2015-05-08T01:08:34Z
Promoter: John Anderson.
RBS: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
Released HQ 2013
Constitutive promoter (strong) with RBS (strong, efficiency=0.3)
false
true
_227_
0
2583
9
In stock
true
We used BioBrick Standard Assembly.
true
Lorenzo Pasotti, Paolo Magni
component1981863
1
BBa_J23100
component1981865
1
BBa_B0030
annotation1981865
1
BBa_B0030
range1981865
1
44
58
annotation1981863
1
BBa_J23100
range1981863
1
1
35
BBa_K531011
1
BBa_K531011
Constitutive promoter and RBS BBa_K081005 + <i>Caulobacter</i> optimized <i>esp</I> + C-terminal sec
2011-09-27T11:00:00Z
2015-05-08T01:12:36Z
BBa_081005 was submitted to the registry by 2008 iGEM Team UNIV_Pavia, codon optimized <I>esp</i> was synthesized by Integrated DNA Technologies, <i>rsaA</i> C-terminal secretion tag was copied from <i>Caulobacter</i> lab strain CB15N.
Produces and secretes active Esp with intact C-terminal secretion tag from RsaA. Esp is a suspected serine protease that breaks up <i>Staphylococcus aureus</i> biofilms.
false
false
_697_
0
8840
9
Not in stock
false
Optimized <i>esp</I> does not have a stop codon to ensure expression of the fused secretion tag.
false
Alexander Aaring
component2146140
1
BBa_K081005
component2146141
1
BBa_K531006
component2146142
1
BBa_K531001
annotation2146140
1
BBa_K081005
range2146140
1
1
58
annotation2146142
1
BBa_K531001
range2146142
1
940
1299
annotation2146141
1
BBa_K531006
range2146141
1
67
931
BBa_B0030
1
BBa_B0030
RBS.1 (strong) -- modified from R. Weiss
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_44_46_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7025
1
BBa_B0030
range7025
1
1
15
annotation1701
1
RBS-1\Strong
range1701
1
1
15
annotation1702
1
RBS
range1702
1
8
12
BBa_K531001
1
BBa_K531001
<i>C. crescentus</i> S-layer RsaA C-term
2011-07-03T11:00:00Z
2015-05-08T01:12:36Z
This comes from genomic ''Caulobacter'' CB15N.
This is a secretion tag for secretion via a Type I secretion pathway in ''Caulobacter crescentus''. It should be appended to the C-terminal end of another protein to act as a secretion tag.
false
false
_697_
0
8840
9
In stock
true
Using the Registry's suggested primer combinations results in the formation of an in sequence stop codon between two coding regions. A one nucleotide change was necessary in both the prefix of this coding sequence and the suffix of the coding region to be added in front of this gene to ensure that their is no stop codon in sequence.
false
Alexander Aaring
BBa_J23100
1
BBa_J23100
constitutive promoter family member
2006-08-03T11:00:00Z
2015-08-31T04:08:40Z
Isolated from library of promoters
Released HQ 2013
Replace later
false
true
_52_
0
483
95
In stock
true
N/A
true
John Anderson
BBa_K531006
1
BBa_K531006
Optimized esp gene for <i>C. crescentus</i>
2011-07-25T11:00:00Z
2015-05-08T01:12:36Z
Wild type esp sequence from Zhang,Y.Q., Ren,S.X., Li,H.L., Wang,Y.X., Fu,G., Yang,J., Qin,Z.Q., Miao,Y.G., Wang,W.Y., Chen,R.S., Shen,Y., Chen,Z., Yuan,Z.H., Zhao,G.P., Qu,D., Danchin,A. and Wen,Y.M. Genome-based analysis of virulence genes in a non-biofilm-forming Staphylococcus epidermidis strain (ATCC 12228). Mol. Microbiol. 49 (6), 1577-1593 (2003). Optimized sequence is based on the wild type sequence.
The wild Type esp is secreted by S. epidermidis, a bacteria that has low G/C content in its genome. It is then optimized to give a high G/C content without changing any amino acids sequence.
false
false
_697_
0
8860
9
It's complicated
false
DNA codons are optimized for <i>C. crescentus</i> expression.
false
Qimeng Gao
BBa_J23100_sequence
1
ttgacggctagctcagtcctaggtacagtgctagc
BBa_K081005_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaa
BBa_B0030_sequence
1
attaaagaggagaaa
BBa_K531011_sequence
1
ttgacggctagctcagtcctaggtacagtgctagctactagagattaaagaggagaaatactagagtgaagaagcgcttcctgtcgatctgcaccatgaccatcgccgccctggccacgacgaccatggtgaacacgagctatgcgaagaccgacaccgagtcccacaaccactcgtcgctgggtaccgaaaacaagaatgtcctggacatcaacagcagctcccacaacatcaagccgagccagaacaagagctatccgagcgtgatcctgccgaataacaaccgccaccagatcttcaacacgacccaagggcactatgacgccgtgtcgttcatctacatcccgatcgacggcggctacatcccgatcgacggcggctacatgagcggctccggcgtcgtcgtcggcgaaacgagatcctcacgaataagcacgtcgtgaacggcgcgaagggcaacccccggaatatctccgtccatccctcggccaagaacgagaacgattatcccaatggtaagttcgtgggccaagaaatcatcccgtaccccggtaactcggacctggccatcctgcgcgtgagccccaacgagcacaatcaacacatcggccaggtggtcaagcccgcgacgatcagcagcaacacggacacccgcatcaacgagaacatcaccgtcaccggctaccccggcgacaagcccctggcgaccatgtgggagagcgtcggcaaggtggtctatatcggcggcgaggagctgcgctatgacctcagcacggtgggcggcaacagcggctcgcccgtcttcaacggcaagaaccaggtgatcggcatccactatggtggtgtcgacaacaagtataactcgagcgtctacatcaatgacttcgtgcagcaattcctgcgcaacaacatcccggacatcaacatccagtactagaggatatcaacgctatcggcacctcgaccgctttcgtgacgatcaccgacgccgctgtcggcgacaagctcgacctcgtcggcatctcgacgaacggcgctatcgctgacggcgccttcggcgctgcggtcaccctgggcgctgctgcgaccctggctcagtacctggacgctgctgctgccggcgacggcagcggcacctcggttgccaagtggttccagttcggcggcgacacctatgtcgtcgttgacagctcggctggcgcgaccttcgtcagcggcgctgacgcggtgatcaagctgaccggtctggtcacgctgaccacctcggccttcgccaccgaagtcctgacgctcgcctaa
BBa_K531006_sequence
1
tgaagaagcgcttcctgtcgatctgcaccatgaccatcgccgccctggccacgacgaccatggtgaacacgagctatgcgaagaccgacaccgagtcccacaaccactcgtcgctgggtaccgaaaacaagaatgtcctggacatcaacagcagctcccacaacatcaagccgagccagaacaagagctatccgagcgtgatcctgccgaataacaaccgccaccagatcttcaacacgacccaagggcactatgacgccgtgtcgttcatctacatcccgatcgacggcggctacatcccgatcgacggcggctacatgagcggctccggcgtcgtcgtcggcgaaacgagatcctcacgaataagcacgtcgtgaacggcgcgaagggcaacccccggaatatctccgtccatccctcggccaagaacgagaacgattatcccaatggtaagttcgtgggccaagaaatcatcccgtaccccggtaactcggacctggccatcctgcgcgtgagccccaacgagcacaatcaacacatcggccaggtggtcaagcccgcgacgatcagcagcaacacggacacccgcatcaacgagaacatcaccgtcaccggctaccccggcgacaagcccctggcgaccatgtgggagagcgtcggcaaggtggtctatatcggcggcgaggagctgcgctatgacctcagcacggtgggcggcaacagcggctcgcccgtcttcaacggcaagaaccaggtgatcggcatccactatggtggtgtcgacaacaagtataactcgagcgtctacatcaatgacttcgtgcagcaattcctgcgcaacaacatcccggacatcaacatccag
BBa_K531001_sequence
1
gatatcaacgctatcggcacctcgaccgctttcgtgacgatcaccgacgccgctgtcggcgacaagctcgacctcgtcggcatctcgacgaacggcgctatcgctgacggcgccttcggcgctgcggtcaccctgggcgctgctgcgaccctggctcagtacctggacgctgctgctgccggcgacggcagcggcacctcggttgccaagtggttccagttcggcggcgacacctatgtcgtcgttgacagctcggctggcgcgaccttcgtcagcggcgctgacgcggtgatcaagctgaccggtctggtcacgctgaccacctcggccttcgccaccgaagtcctgacgctcgcctaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z