BBa_K533004 1 BBa_K533004 OmpA-mCherry 2011-09-28T11:00:00Z 2015-05-08T01:12:36Z OmpA is cloned from genomic DNA of BL21(DE3) E. Coli strain. mCherry is from the Clontech plasmid pmCherry-C1. This is designed as the positive control for protein presentation on the outer membrane. false false _699_ 0 6884 9 It's complicated false His tag is inserted between OmpA and mCherry to facilitate detection after expression. false ZHANG Yunxiao annotation2150606 1 lacI range2150606 1 20 44 annotation2150611 1 mCherry range2150611 1 699 1409 annotation2150605 1 T7 range2150605 1 1 18 annotation2150607 1 rbs range2150607 1 75 79 annotation2150610 1 His6Tag range2150610 1 648 665 annotation2150612 1 stop range2150612 1 1407 1409 annotation2150609 1 OmpA range2150609 1 87 633 annotation2150608 1 ATG range2150608 1 87 89 BBa_K533004_sequence 1 taatacgactcactataggggaattgtgagcggataacaattccccctagaaataattttgtttaactttaagaaggagatataccatggcaatgaaaaagacagctatcgcgattgcagtggcactggctggtttcgctaccgtagcgcaggccgctccgaaagataacacctggtacactggtgctaaactgggctggtcccagtaccatgacactggtttcatcaacaacaatggcccgacccatgaaaaccaactgggcgctggtgcttttggtggttaccaggttaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaactccatgggcagcagccatcatcatcatcatcacagcaccggcctggtgccgcgcggcagccatatgatggtgagcaagggcgaggaggataacatggccatcatcaaggagttcatgcgcttcaaggtgcacatggagggctccgtgaacggccacgagttcgagatcgagggcgagggcgagggccgcccctacgagggcacccagaccgccaagctgaaggtgaccaagggtggccccctgcccttcgcctgggacatcctgtcccctcagttcatgtacggctccaaggcctacgtgaagcaccccgccgacatccccgactacttgaagctgtccttccccgagggcttcaagtgggagcgcgtgatgaacttcgaggacggcggcgtggtgaccgtgacccaggactcctccctgctggacggcgagttcatctacaaggtgaagctgcgcggcaccaacttcccctccgacggccccgtaatgcagaagaagaccatgggctgggaggcctcctccgagcggatgtaccccgaggacggcgccctgaagggcgagatcaagcagaggctgaagctgaaggacggcggccactacgacgctgaggtcaagaccacctacaaggccaagaagcccgtgcagctgcccggcgcctacaacgtcaacatcaagttggacatcacctcccacaacgaggactacaccatcgtggaacagtacgaacgcgccgagggccgccactccaccggcggcatggacgagctgtacaagtagggatccggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z