BBa_K533005 1 BBa_K533005 OmpA-GFP 2011-09-28T11:00:00Z 2015-05-08T01:12:36Z OmpA is cloned from BL21(DE3) E. Coli strain. GFP is from Clontech plasmid pGFP-N1 GFP is presented outside E. Coli as a positive control for protein presentation false false _699_ 0 6884 9 It's complicated true His tag is inserted between OmpA and GFP for easier detection in Western Blotting. false ZHANG Yunxiao annotation2150601 1 lacI range2150601 1 20 44 annotation2150602 1 T7 range2150602 1 1 18 annotation2150600 1 His Tag range2150600 1 648 665 annotation2150597 1 OmpA range2150597 1 87 633 annotation2150603 1 stop range2150603 1 1416 1418 annotation2150599 1 rbs range2150599 1 75 79 annotation2150604 1 start range2150604 1 87 89 annotation2150598 1 GFP range2150598 1 696 1418 BBa_K533005_sequence 1 taatacgactcactataggggaattgtgagcggataacaattccccctagaaataattttgtttaactttaagaaggagatataccatggcaatgaaaaagacagctatcgcgattgcagtggcactggctggtttcgctaccgtagcgcaggccgctccgaaagataacacctggtacactggtgctaaactgggctggtcccagtaccatgacactggtttcatcaacaacaatggcccgacccatgaaaaccaactgggcgctggtgcttttggtggttaccaggttaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaactccatgggcagcagccatcatcatcatcatcacagcaccggcctggtgccgcgcggcagccatatgatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtagggatccggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z