BBa_K533005
1
BBa_K533005
OmpA-GFP
2011-09-28T11:00:00Z
2015-05-08T01:12:36Z
OmpA is cloned from BL21(DE3) E. Coli strain. GFP is from Clontech plasmid pGFP-N1
GFP is presented outside E. Coli as a positive control for protein presentation
false
false
_699_
0
6884
9
It's complicated
true
His tag is inserted between OmpA and GFP for easier detection in Western Blotting.
false
ZHANG Yunxiao
annotation2150601
1
lacI
range2150601
1
20
44
annotation2150602
1
T7
range2150602
1
1
18
annotation2150600
1
His Tag
range2150600
1
648
665
annotation2150597
1
OmpA
range2150597
1
87
633
annotation2150603
1
stop
range2150603
1
1416
1418
annotation2150599
1
rbs
range2150599
1
75
79
annotation2150604
1
start
range2150604
1
87
89
annotation2150598
1
GFP
range2150598
1
696
1418
BBa_K533005_sequence
1
taatacgactcactataggggaattgtgagcggataacaattccccctagaaataattttgtttaactttaagaaggagatataccatggcaatgaaaaagacagctatcgcgattgcagtggcactggctggtttcgctaccgtagcgcaggccgctccgaaagataacacctggtacactggtgctaaactgggctggtcccagtaccatgacactggtttcatcaacaacaatggcccgacccatgaaaaccaactgggcgctggtgcttttggtggttaccaggttaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaactccatgggcagcagccatcatcatcatcatcacagcaccggcctggtgccgcgcggcagccatatgatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgacctacggcgtgcagtgcttcagccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactacaacagccacaacgtctatatcatggccgacaagcagaagaacggcatcaaggtgaacttcaagatccgccacaacatcgaggacggcagcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccgccctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtagggatccggctgctgccaccgctgagcaataactagcataaccccttggggcctctaaacgggtcttgaggggttttttg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z