LOCUS BBa_K533010 3750 bp DNA linear 17-May-2024 DEFINITION PAA receptor weakened expression cassette ACCESSION BBa_K533010 VERSION BBa_K533010.1 FEATURES Location/Qualifiers promoter 585..590 /label=weak -10 promoter 565..570 /label=weak -35 CDS 655..2316 /label=PAA Receptor CDS 2867..3727 /label=bla CDS 165..662 /label=incomplete lacI misc_feature 660..662 /label=lacI stop promoter 85..90 /label=-35 ORIGIN 1 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agcagatcaa ttcgcgcgcg 61 aaggcgaagc ggcatgcatt tacgttgaca ccatcgaatg gtgcaaaacc tttcgcggta 121 tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt 181 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc 241 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga 301 attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg 361 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc 421 gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag 481 cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact 541 atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt 601 tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgatggct 661 aaccgtatcc gcgtagtcac gctgttggta atggtgctgg gggtattcgc actgatacag 721 cttatttccg gctgtctgat tttttctccc cttcaccata gccagaagaa ctttgaggtt 781 tgcaatcaat tacgggaaca gcagggcgag ctgacgtcaa cctgggattt aatgctgcaa 841 acgcgcatta acctgagtcg ttcagcggta tggatgatgg tggattcctc caatcaacaa 901 agtaacgcca aagttgaatt gctcgatagc gccaggaaaa cattggcgca ggcatcgacg 961 cattataaaa aattcaaaag catggcaccg ttacctgaaa tggtcgctac cagtcgtaat 1021 attgatgaaa aatataaaaa ctattacaca gcgttatctg gactgattga ttatctggat 1081 aatggcaata ctggagcttt tctcgctcat ccagctcagg gaatgcaaga tgcaatgggc 1141 gaagcgcttg ctcagtacgc cctcagcagt gaaaaactgt atcgcgactt cgtcactgtc 1201 aacgcagatg attaccgatt tgcccagtgg caactggcgg ctatcgcgct ggtggtggta 1261 ttgattctgc tcgtggcgtg gtacggcatt cgccgtatgt tgcttactcc gctggcaaaa 1321 attattgctc acattcgcga aatcgccggt ggtaacctgg cgaataccct gaccattgac 1381 gggcgcagtg aaatgggcgt cctggcgcag agcattacac atatgcaacg ctctttgact 1441 gacaccgtca ctcatgtccg cgaaggttca gatgccatct atgccggtac ccgtgaaatt 1501 gcggcgggca acaccgatct ttcctcccgt actgaacagc aggcatccgc gctggaagaa 1561 actgccgcca gcatggagca gctcaccgcg acagtgaagc aaaacgccga taacgcccgc 1621 caggcctcgc aactggcgca aagtgcctcc gacaccgccc agcacggcgg caaagtggtg 1681 gatggcgtag tgaaaacgat gcatgagatc gccggattgg tcgaagaaaa ttgccgacat 1741 tatcagcgtt atcgacggta ttgccttcca gactactatc ctcgcgctga atgccgcggt 1801 tgcgccgcgc gtgcgggtga acagggccgt ggttttgccg tggtggcggg tgaagtgcgt 1861 aatcttgcca gtcgcagcgc ccaggcggca aaagagatca aagccctcat tgaagactcc 1921 gtctcacgcg ttgataccgg ttcggtgctg gtcgaaagcg ccggggaaac aatgaacaat 1981 atcgtcaatg ctgtcactcg cgtgactgac attatgggcg agattgcatc ggcatcggat 2041 gaacagagcc gtggcatcga tcaagtcgca ttggcggttt cggaaatgga tcgcgtcacg 2101 caacagaacg catcgctggt gcaggaatca gctgccgccg ccgctgcgct ggaagaacag 2161 gcgagtcgtt taacgcaagc agtttccgcg ttccgtctgg cagccagccc actcaccaat 2221 aaaccgcaaa caccatcccg tcctgccagt gagcaaccac cggctcagcc acgactgcga 2281 attgctgaac aagatccaaa ctgggaaaca ttttgacgag tatttactaa cgcggtcatt 2341 gaagcttggc tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga 2401 acgcagaagc ggtctgataa aacagaattt gcctggcggc agtagcgcgg tggtcccacc 2461 tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc 2521 ccatgcgaga gtagggaact gccaggcatc aaataaaacg aaaggctcag tcgaaagact 2581 gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc 2641 cgggagcgga tttgaacgtt gcgaagcaac ggcccggagg gtggcgggca ggacgcccgc 2701 cataaactgc caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt 2761 ttctacaaac tctttttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 2821 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca 2881 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc 2941 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat 3001 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc 3061 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg 3121 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc 3181 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat 3241 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga 3301 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc 3361 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgccta cagcaatggc 3421 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt 3481 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc 3541 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc 3601 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca 3661 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca 3721 ttggtaactg tcagaccaag tttactcata //