LOCUS       BBa_K533012             2602 bp    DNA     linear       08-Jan-2025
DEFINITION  safety version of pSB1C3 plasmid
ACCESSION   BBa_K533012
VERSION     BBa_K533012.1  
FEATURES             Location/Qualifiers
     CDS             2061..2366
                     /label=ccdB
     misc_feature    2590..2595
                     /label=new EcoRI site
     misc_feature    2049..2054
                     /label=mutated EcoRI
     promoter        2527..2532
                     /label=-10
     promoter        2558..2563
                     /label=-35
     stem_loop       2022..2041
                     /label=stem_loop
     misc_feature    261..874
                     /label=pMB1
     CDS             1109..1819
                     /label=Chloramphenicol resistance
     stem_loop       22..93
                     /label=E. coli his operon terminator
     misc_feature    2571..2588
                     /label=mutated I-SceI site
     primer_bind     157..176
                     /label=VR
     primer_bind     1933..1952
                     /label=VF2
ORIGIN
        1 tactagtagc ggccgctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt
       61 ttctttaaaa ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct
      121 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      181 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      241 caggaaccgt aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga
      301 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      361 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      421 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      481 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
      541 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
      601 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
      661 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
      721 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      781 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
      841 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
      901 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
      961 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1021 ttggtctgac agctcgaggc ttggattctc accaataaaa aacgcccggc ggcaaccgag
     1081 cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca acaggagtcc
     1141 aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac tgttgtaatt
     1201 cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc tgaatcgcca
     1261 gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa acgggggcga
     1321 agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc cagggattgg
     1381 ctgagacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg ttttcaccgt
     1441 aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg tggtattcac
     1501 tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa gggtgaacac
     1561 tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga tgagcattca
     1621 tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt ttctttacgg
     1681 tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat tgagcaactg
     1741 actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg gtggtatatc
     1801 cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat aactcaaaaa
     1861 atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt acgtgcccga
     1921 tcaactcgag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     1981 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
     2041 gatttctgga attgaagctt ttatattccc cagaacatca ggttaatggc gtttttgatg
     2101 tcattttcgc ggtggctgag atcagccact tcttccccga taacggagac cggcacactg
     2161 gccatatcgg tggtcatcat gcgccagctt tcatccccga tatgcaccac cgggtaaagt
     2221 tcacgggaga ctttatctga cagcagacgt gcactggcca gggggatcac catccgtcgc
     2281 ccgggcgtgt caataatatc actctgtaca tccacaaaca gacgataacg gctctctctt
     2341 ttataggtgt aaaccttaaa ctgcatttca ccagcccctg ttctcgtcag caaaagagcc
     2401 gttcatttca ataaaccggg cgacctcagc catcccttcc tgattttccg ctttccagcg
     2461 ttcggcacgc agacgacggg cttcattctg catggttgtg cttaccagac cggagatatt
     2521 gacatcatat atgccttgag caactgatag ctgtcgctgt caaccggatc ctagagataa
     2581 cagggtaatg aattctctag ag
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