##gff-version 3
##sequence-region BBa_K533012 22 2595
BBa_K533012	.	CDS	2061	2366	.	+	0	ID=annotation2161040;Name=ccdB
BBa_K533012	.	promoter	2558	2563	.	+	0	ID=annotation2161044;Name=-35
BBa_K533012	.	primer_binding_site	157	176	.	+	0	ID=annotation2161082;Name=VR
BBa_K533012	.	stem_loop	22	93	.	+	0	ID=annotation2161048;Name=E. coli his operon terminator
BBa_K533012	.	sequence_feature	2049	2054	.	+	0	ID=annotation2161042;Name=mutated EcoRI
BBa_K533012	.	promoter	2527	2532	.	+	0	ID=annotation2161043;Name=-10
BBa_K533012	.	primer_binding_site	1933	1952	.	+	0	ID=annotation2161083;Name=VF2
BBa_K533012	.	stem_loop	2022	2041	.	+	0	ID=annotation2161045;Name=stem_loop
BBa_K533012	.	sequence_feature	261	874	.	+	0	ID=annotation2161046;Name=pMB1
BBa_K533012	.	sequence_feature	2571	2588	.	+	0	ID=annotation2161049;Name=mutated I-SceI site
BBa_K533012	.	CDS	1109	1819	.	+	0	ID=annotation2161047;Name=Chloramphenicol resistance
BBa_K533012	.	sequence_feature	2590	2595	.	+	0	ID=annotation2161041;Name=new EcoRI site
>BBa_K533012
tactagtagcggccgctgcagtccggcaaaaaagggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaaga
ttacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctc
actcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaa
ggccaggaaccgtaaaaaggccgcgttgctggcgtttttccacaggctccgcccccctgacgagcatcacaaaaatcga
cgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgct
ctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctc
acgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgac
cgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactg
gtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactag
aagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaa
caaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatc
ctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaa
aaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtct
gacagctcgaggcttggattctcaccaataaaaaacgcccggcggcaaccgagcgttctgaacaaatccagatggagtt
ctgaggtcattactggatctatcaacaggagtccaagcgagctcgatatcaaattacgccccgccctgccactcatcgc
agtactgttgtaattcattaagcattctgccgacatggaagccatcacaaacggcatgatgaacctgaatcgccagcgg
catcagcaccttgtcgccttgcgtataatatttgcccatggtgaaaacgggggcgaagaagttgtccatattggccacg
tttaaatcaaaactggtgaaactcacccagggattggctgagacgaaaaacatattctcaataaaccctttagggaaat
aggccaggttttcaccgtaacacgccacatcttgcgaatatatgtgtagaaactgccggaaatcgtcgtggtattcact
ccagagcgatgaaaacgtttcagtttgctcatggaaaacggtgtaacaagggtgaacactatcccatatcaccagctca
ccgtctttcattgccatacgaaattccggatgagcattcatcaggcgggcaagaatgtgaataaaggccggataaaact
tgtgcttatttttctttacggtctttaaaaaggccgtaatatccagctgaacggtctggttataggtacattgagcaac
tgactgaaatgcctcaaaatgttctttacgatgccattgggatatatcaacggtggtatatccagtgatttttttctcc
attttagcttccttagctcctgaaaatctcgataactcaaaaaatacgcccggtagtgatcttatttcattatggtgaa
agttggaacctcttacgtgcccgatcaactcgagtgccacctgacgtctaagaaaccattattatcatgacattaacct
ataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattg
aagcttttatattccccagaacatcaggttaatggcgtttttgatgtcattttcgcggtggctgagatcagccacttct
tccccgataacggagaccggcacactggccatatcggtggtcatcatgcgccagctttcatccccgatatgcaccaccg
ggtaaagttcacgggagactttatctgacagcagacgtgcactggccagggggatcaccatccgtcgcccgggcgtgtc
aataatatcactctgtacatccacaaacagacgataacggctctctcttttataggtgtaaaccttaaactgcatttca
ccagcccctgttctcgtcagcaaaagagccgttcatttcaataaaccgggcgacctcagccatcccttcctgattttcc
gctttccagcgttcggcacgcagacgacgggcttcattctgcatggttgtgcttaccagaccggagatattgacatcat
atatgccttgagcaactgatagctgtcgctgtcaaccggatcctagagataacagggtaatgaattctctagag