LOCUS       BBa_K535000             3552 bp    DNA     linear       27-Dec-2024
DEFINITION  HydEF -> C. reinhardtii
ACCESSION   BBa_K535000
VERSION     BBa_K535000.1  
FEATURES             Location/Qualifiers
     promoter        1..38
                     /label=pLacZ
     misc_feature    39..41
                     /label=TSS
     misc_feature    42..59
                     /label=spacer
     RBS             60..66
                     /label=RBS
     misc_feature    67..93
                     /label=spacer
     CDS             94..3546
                     /label=HydEF
     misc_feature    3547..3552
                     /label=double terminator
     misc_feature    73..93
                     /label=insertion
ORIGIN
        1 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aggatgaaaa aagtaataaa
       61 ggaggtttaa agatggcgca ttccctgtcg gcgatggccc attcgctgtc ggcccattcg
      121 cgccaggccg gcgaccgcaa gctgggcgcc ggcgccgcct cgtcgcgccc gtcgtgcccg
      181 tcgcgccgca tcgtccgcgt cgccgcccat gcctcggcct cgaaggccac cccggacgtc
      241 ccggtcgacg acctgccgcc ggcccatgcc cgcgccgccg tcgccgccgc caaccgccgc
      301 gcccgcgcca tggcctcggc cgaggccgcc gccgagaccc tgggcgactt cctgggcctg
      361 ggcaagggcg gcctgtcgcc gggcgccacc gccaacctgg accgcgagca ggtcctgggc
      421 gtcctggagg ccgtctggcg ccgcggcgac ctgaacctgg agcgcgccct gtattcgcat
      481 gccaacgccg tcaccaacaa gtattgcggc ggcggcgtct attatcgcgg cctggtcgag
      541 ttctcgaaca tctgccagaa cgactgctcg tattgcggca tccgcaacaa ccagaaggag
      601 gtctggcgct ataccatgcc ggtcgaggag gtcgtcgagg tcgccaagtg ggccctggag
      661 aacggcatcc gcaacatcat gctccagggc ggcgagctga agaccgagca gcgcctggcc
      721 tatctggagg cctgcgtccg cgccatccgc gaggagacca cccagctgga cctggagatg
      781 cgcgcccgcg ccgcctcgac caccaccgcc gaggccgccg cctcggccca ggccgacgcc
      841 gaggccaagc gcggcgagcc ggagctgggc gtcgtcgtct cgctgtcggt cggcgagctg
      901 ccgatggagc agtatgagcg cctgttccgc gccggcgccc gccgctatct gatccgcatc
      961 gagacctcga acccggacct gtatgccgcc ctgcatccgg agccgatgtc gtggcatgcc
     1021 cgcgtcgagt gcctgcgcaa cctgaagaag gccggctata tgctgggcac cggcgtcatg
     1081 gtcggcctgc cgggccagac cctgcatgac ctggccggcg acgtcatgtt cttccgcgac
     1141 atcaaggccg acatgatcgg catgggcccg ttcatcaccc agccgggcac cccggccacc
     1201 gacaagtgga ccgccctgta tccgaacgcc aacaagaact cgcatatgaa gtcgatgttc
     1261 gacctgacca ccgccatgaa cgccctggtc cgcatcacca tgggcaacgt caacatctcg
     1321 gccaccaccg ccctccaggc catcatcccg accggccgcg agatcgccct ggagcgcggc
     1381 gccaacgtcg tcatgccgat cctgaccccg acccagtatc gcgagtcgta tcagctgtat
     1441 gagggcaagc cgtgcatcac cgacaccgcc gtccagtgcc gccgctgcct ggacatgcgc
     1501 ctgcattcgg tcggcaagac ctcggccgcc ggcgtctggg gcgacccggc ctcgttcctg
     1561 catccgatcg tcggcgtccc ggtcccgcat gacctgtcgt cgccggccct ggccgccgcc
     1621 gcctcggccg acttccatga ggtcggcgcc ggcccgtgga acccgatccg cctggagcgc
     1681 ctggtcgagg tcccggaccg ctatccggac ccggacaacc atggccgcaa gaaggccggc
     1741 gccggcaagg gcggcaaggc ccatgactcg catgacgacg gcgaccatga cgaccatcat
     1801 catcatcatg gcgccgcccc ggccggcgcc gccgccggca agggcaccgg cgccgccgcc
     1861 atcggcggcg gcgccggcgc ctcgcgccag cgcgtcgccg gcgccgccgc cgcctcggcc
     1921 cgcctgtgcg ccggcgcccg ccgcgccggc cgcgtcgtcg cctcgccgct gcgcccggcc
     1981 gccgcctgcc gcggcgtcgc cgtcaaggcc gccgccgccg ccgccggcga ggacgccggc
     2041 gccggcacct cgggcgtcgg ctcgaacatc gtcacctcgc cgggcatcgc ctcgaccacc
     2101 gcccatggcg tcccgcgcat caacatcggc gtcttcggcg tcatgaacgc cggcaagtcg
     2161 accctggtca acgccctggc ccagcaggag gcctgcatcg tcgactcgac cccgggcacc
     2221 accgccgacg tcaagaccgt cctgctggag ctgcatgccc tgggcccggc caagctgctg
     2281 gacaccgccg gcctggacga ggtcggcggc ctgggcgaca agaagcgccg caaggccctg
     2341 aacaccctga aggagtgcga cgtcgccgtc ctggtcgtcg acaccgacac cgccgccgcc
     2401 gccatcaagt cgggccgcct ggccgaggcc ctggagtggg agtcgaaggt catggagcag
     2461 gcccataagt ataacgtctc gccggtcctg ctgctgaacg tcaagtcgcg cggcctgccg
     2521 gaggcccagg ccgcctcgat gctggaggcc gtcgccggca tgctggaccc gtcgaagcag
     2581 atcccgcgca tgtcgctgga cctggcctcg accccgctgc atgagcgctc gaccatcacc
     2641 tcggccttcg tcaaggaggg cgccgtccgc tcgtcgcgct atggcgcccc gctgccgggc
     2701 tgcctgccgc gctggtcgct gggccgcaac gcccgcctgc tgatggtcat cccgatggac
     2761 gccgagaccc cgggcggccg cctgctgcgc ccgcaggccc aggtcatgga ggaggccatc
     2821 cgccattggg ccaccgtcct gtcggtccgc ctggacctgg acgccgcccg cggcaagctg
     2881 ggcccggagg cctgcgagat ggagcgccag cgcttcgacg gcgtcatcgc catgatggag
     2941 cgcaacgacg gcccgaccct ggtcgtcacc gactcgcagg ccatcgacgt cgtccatccg
     3001 tggaccctgg accgctcgtc gggccgcccg ctggtcccga tcaccacctt ctcgatcgcc
     3061 atggcctatc agcagaacgg cggccgcctg gacccgttcg tcgagggcct ggaggccctg
     3121 gagaccctcc aggacggcga ccgcgtcctg atctcggagg cctgcaacca taaccgcatc
     3181 acctcggcct gcaacgacat cggcatggtc cagatcccga acaagctgga ggccgccctg
     3241 ggcggcaaga agctccagat cgagcatgcc ttcggccgcg agttcccgga gctggagtcg
     3301 ggcggcatgg acggcctgaa gctggccatc cattgcggcg gctgcatgat cgacgcccag
     3361 aagatgcagc agcgcatgaa ggacctgcat gaggccggcg tcccggtcac caactatggc
     3421 gtcttcttct cgtgggccgc ctggccggac gccctgcgcc gcgccctgga gccgtggggc
     3481 gtcgagccgc cggtcggcac cccggccacc ccggccgccg ccccggccac cgccgcctcg
     3541 ggcgtctaat aa
//