LOCUS       BBa_K535005             2347 bp    DNA     linear       28-Dec-2024
DEFINITION  HydA1 - FeOx construction (Hydrogenase-ferredoxin fussion)
ACCESSION   BBa_K535005
VERSION     BBa_K535005.1  
FEATURES             Location/Qualifiers
     promoter        1..203
                     /label=nifH promoter
     misc_feature    19..35
                     /label=Enhancer binding site
     misc_feature    125..141
                     /label=-24/-12 box
     promoter        1..203
                     /label=BBa_K535004
     misc_feature    210..347
                     /label=Periplasm export tag
     CDS             348..2093
                     /label=HydA
     CDS             210..2093
                     /label=BBa_K535002
     misc_feature    2102..2143
                     /label=linker
     CDS             2144..2311
                     /label=FeOx
     misc_feature    2312..2341
                     /label=poly His tag
     misc_feature    2342..2347
                     /label=double TAA stop
     CDS             2102..2347
                     /label=BBa_K535003
ORIGIN
        1 cgacaccgtc tgtcggcttt gtctgatcgg cgacattagg tttgtttggc agtttcctgt
       61 ggtggttcgg agtaactttc tgaaacccaa caaaaggatc tttcctttgg ctcgatcggc
      121 ccacatggca cgggttttga agattgccat gcgaggcggc gcgagctgcc tgccttttac
      181 tcagcgatgg atggaacgaa agatactaga tgccgtcgta ccgccccccg aagatcgcct
      241 cgtcggagat caccccgcgc caggtctatc tgcgccgccg cgagttcctg ggcgccgcca
      301 ccctgggcgc catggccctg tatggcgccg gcaaggcctc ggcccatatg aagaccatca
      361 tcctgaacgg caacgaggtc cataccgaca aggacatcac catcctggag ctggcccgcg
      421 agaacaacgt cgacatcccg accctgtgct tcctgaagga ctgcggcaac ttcggcaagt
      481 gcggcgtctg catggtcgag gtcgagggca agggcttccg cgccgcctgc gtcgccaagg
      541 tcgaggacgg catggtcatc aacaccgagt cggacgaggt caaggagcgc atcaagaagc
      601 gcgtctcgat gctgctggac aagcatgagt tcaagtgcgg ccagtgctcg cgccgcgaga
      661 actgcgagtt cctgaagctg gtcatcaaga ccaaggccaa ggcctcgaag ccgttcctgc
      721 cggaggacaa ggacgccctg gtcgacaacc gctcgaaggc catcgtcatc gaccgctcga
      781 agtgcgtcct gtgcggccgc tgcgtcgccg cctgcaagca gcatacctcg acctgctcga
      841 tccagttcat caagaaggac ggccagcgcg ccgtcggcac cgtcgacgac gtctgcctgg
      901 acgactcgac ctgcctgctg tgcggccagt gcgtcatcgc ctgcccggtc gccgccctga
      961 aggagaagtc gcatatcgag aaggtccagg aggccctgaa cgacccgaag aagcatgtca
     1021 tcgtcgccat ggccccgtcg gtccgcaccg ccatgggcga gctgttcaag atgggctatg
     1081 gcaaggacgt caccggcaag ctgtataccg ccctgcgcat gctgggcttc gacaaggtct
     1141 tcgacatcaa cttcggcgcc gacatgacca tcatggagga ggccaccgag ctgctgggcc
     1201 gcgtcaagaa caacggcccg ttcccgatgt tcacctcgtg ctgcccggcc tgggtccgcc
     1261 tggcccagaa ctatcatccg gagctgctgg acaacctgtc gtcggccaag tcgccgcagc
     1321 agatcttcgg caccgcctcg aagacctatt atccgtcgat ctcgggcatc gccccggagg
     1381 acgtctatac cgtcaccatc atgccgtgca acgacaagaa gtatgaggcc gacatcccgt
     1441 tcatggagac caactcgctg cgcgacatcg acgcctcgct gaccacccgc gagctggcca
     1501 agatgatcaa ggacgccaag atcaagttcg ccgacctgga ggacggcgag gtcgacccgg
     1561 ccatgggcac ctattcgggc gccggcgcca tcttcggcgc caccggcggc gtcatggagg
     1621 ccgccatccg ctcggccaag gacttcgccg agaacaagga gctggagaac gtcgactata
     1681 ccgaggtccg cggcttcaag ggcatcaagg aggccgaggt cgagatcgcc ggcaacaagc
     1741 tgaacgtcgc cgtcatcaac ggcgcctcga acttcttcga gttcatgaag tcgggcaaga
     1801 tgaacgagaa gcagtatcat ttcatcgagg tcatggcctg cccgggcggc tgcatcaacg
     1861 gcggcggcca gccgcatgtc aacgccctgg accgcgagaa cgtcgactat cgcaagctgc
     1921 gcgcctcggt cctgtataac caggacaaga acgtcctgtc gaagcgcaag tcgcatgaca
     1981 acccggccat catcaagatg tatgactcgt atttcggcaa gccgggcgag ggcctggccc
     2041 ataagctgct gcatgtcaag tataccaagg acaagaacgt ctcgaagcat gagtactaga
     2101 gggcggcggc tcgggcggcg gcggctcggg cggcggcggc tcgatggcct ataagatcac
     2161 cgacgcctgc gtctcgtgcg gctcgtgcgc ctcggagtgc ccggtctcgg ccatctcgca
     2221 gggcgacacc cagttcgtca tcgacgccga cacctgcatc gagtgcggca actgcgccaa
     2281 cgtctgcccg gtcggcgccc cggtccagga gaagcttcat catcatcatc atcataagct
     2341 ttaataa
//