LOCUS BBa_K535007 5027 bp DNA linear 23-Dec-2024 DEFINITION pBBR1MCS-5 -> broad-host-range cloning vector ACCESSION BBa_K535007 VERSION BBa_K535007.1 FEATURES Location/Qualifiers misc_feature 1..21 /label=BioBrick suffix stem_loop 22..93 /label=E coli his operon terminator stem_loop 30..65 /label=stem loop misc_feature 232..237 /label=sacI restriction site CDS 997..1251 /label=REP CDS 3712..3966 /label=Gentamicine resistance marker misc_feature 4904..4909 /label=apaI restriction site stem_loop 4963..4967 /label=end of terminator stem_loop 4977..4996 /label=stem loop misc_feature 5006..5027 /label=BioBrick prefix ORIGIN 1 tactagtagc ggccgctgca gtccggcaaa aaaacgggca aggtgtcacc accctgccct 61 ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct cactgactcg 121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg cgagctccaa 241 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga 301 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag 361 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa 421 tggcgaatgg aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa 481 atcagctcat tttttaacca ataggccgac tgcgatgagt ggcagggcgg ggcgtaattt 541 ttttaaggca gttattggtg cccttaaacg cctggtgcta cgcctgaata agtgataata 601 agcggatgaa tggcagaaat tcgaaagcaa attcgacccg gtcgtcggtt cagggcaggg 661 tcgttaaata gccgcttatg tctattgctg gtttaccggt ttattgacta ccggaagcag 721 tgtgaccgtg tgcttctcaa atgcctgagg ccagtttgct caggctctcc ccgtggaggt 781 aataattgac gatatgatca tttattctgc ctcccagagc ctgataaaaa cggtgaatcc 841 gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga 901 cgcaacgcgg ggaggcagac aaggtatagg gcggcgaggc ggctacagcc gatagtctgg 961 aacagcgcac ttacgggttg ctgcgcaacc caagtgctac cggcgcggca gcgtgacccg 1021 tgtcggcggc tccaacggct cgccatcgtc cagaaaacac ggctcatcgg gcatcggcag 1081 gcgctgctgc ccgcgccgtt cccattcctc cgtttcggtc aaggctggca ggtctggttc 1141 catgcccgga atgccgggct ggctgggcgg ctcctcgccg gggccggtcg gtagttgctg 1201 ctcgcccgga tacagggtcg ggatgcggcg caggtcgcca tgccccaaca gcgattcgtc 1261 ctggtcgtcg tgatcaacca ccacggcggc actgaacacc gacaggcgca actggtcgcg 1321 gggctggccc cacgccacgc ggtcattgac cacgtaggcc gacacggtgc cggggccgtt 1381 gagcttcacg acggagatcc agcgctcggc caccaagtcc ttgactgcgt attggaccgt 1441 ccgcaaagaa cgtccgatga gcttggaaag tgtcttctgg ctgaccacca cggcgttctg 1501 gtggcccatc tgcgccacga ggtgatgcag cagcattgcc gccgtgggtt tcctcgcaat 1561 aagcccggcc cacgcctcat gcgctttgcg ttccgtttgc acccagtgac cgggcttgtt 1621 cttggcttga atgccgattt ctctggactg cgtggccatg cttatctcca tgcggtaggg 1681 tgccgcacgg ttgcggcacc atgcgcaatc agctgcaact tttcggcagc gcgacaacaa 1741 ttatgcgttg cgtaaaagtg gcagtcaatt acagattttc tttaacctac gcaatgagct 1801 attgcggggg gtgccgcaat gagctgttgc gtacccccct tttttaagtt gttgattttt 1861 aagtctttcg catttcgccc tatatctagt tctttggtgc ccaaagaagg gcacccctgc 1921 ggggttcccc cacgccttcg gcgcggctcc ccctccggca aaaagtggcc cctccggggc 1981 ttgttgatcg actgcgcggc cttcggcctt gcccaaggtg gcgctgcccc cttggaaccc 2041 ccgcactcgc cgccgtgagg ctcggggggc aggcgggcgg gcttcgcctt cgactgcccc 2101 cactcgcata ggcttgggtc gttccaggcg cgtcaaggcc aagccgctgc gcggtcgctg 2161 cgcgagcctt gacccgcctt ccacttggtg tccaaccggc aagcgaagcg cgcaggccgc 2221 aggccggagg cttttcccca gagaaaatta aaaaaattga tggggcaagg ccgcaggccg 2281 cgcagttgga gccggtgggt atgtggtcga aggctgggta gccggtgggc aatccctgtg 2341 gtcaagctcg tgggcaggcg cagcctgtcc atcagcttgt ccagcagggt tgtccacggg 2401 ccgagcgaag cgagccagcc ggtggccgct cgcggccatc gtccacatat ccacgggctg 2461 gcaagggagc gcagcgaccg cgcagggcga agcccggaga gcaagcccgt agggcgccgc 2521 agccgccgta ggcggtcacg actttgcgaa gcaaagtcta gtgagtatac tcaagcattg 2581 agtggcccgc cggaggcacc gccttgcgct gcccccgtcg agccggttgg acaccaaaag 2641 ggaggggcag gcatggcggc atacgcgatc atgcgatgca agaagctggc gaaaatgggc 2701 aacgtggcgg ccagtctcaa gcacgcctac cgcgagcgcg agacgcccaa cgctgacgcc 2761 agcaggacgc cagagaacga gcactgggcg gccagcagca ccgatgaagc gatgggccga 2821 ctgcgcgagt tgctgccaga gaagcggcgc aaggacgctg tgttggcggt cgagtacgtc 2881 atgacggcca gcccggaatg gtggaagtcg gccagccaag aacagcaggc ggcgttcttc 2941 gagaaggcgc acaagtggct ggcggacaag tacggggcgg atcgcatcgt gacggccagc 3001 atccaccgtg acgaaaccag cccgcacatg accgcgttcg tggtgccgct gacgcaggac 3061 ggcaggctgt cggccaagga gttcatcggc aacaaagcgc agatgacccg cgaccagacc 3121 acgtttgcgg ccgctgtggc cgatctaggg ctgcaacggg gcatcgaggg cagcaaggca 3181 cgtcacacgc gcattcaggc gttctacgag gccctggagc ggccaccagt gggccacgtc 3241 accatcagcc cgcaagcggt cgagccacgc gcctatgcac cgcagggatt ggccgaaaag 3301 ctgggaatct caaagcgcgt tgagacgccg gaagccgtgg ccgaccggct gacaaaagcg 3361 gttcggcagg ggtatgagcc tgccctacag gccgccgcag gagcgcgtga gatgcgcaag 3421 aaggccgatc aagcccaaga gacggcccga gaccttcggg agcgcctgaa gcccgttctg 3481 gacgccctgg ggccgttgaa tcgggatatg caggccaagg ccgccgcgat catcaaggcc 3541 gtgggcgaaa agctgctgac ggaacagcgg gaagtccagc gccagaaaca ggcccagcgc 3601 cagcaggaac gcgggcgcgc acatttcccc gaaaagtgcc acctggcggc gttgtgacaa 3661 tttaccgaac aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc 3721 ggtacttggg tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag 3781 agccactgcg ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc 3841 gttggcctca tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc 3901 gccggagact gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag 3961 aacgtaagcc gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa 4021 tgtcttacta cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt 4081 ggctacgtct ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg 4141 gtcagggccg agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt 4201 agggcgactg ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata 4261 acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact 4321 gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc 4381 gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac 4441 gaaccgaaca ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc 4501 catgggcaaa tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca 4561 tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagttttt 4621 atgcatgcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 4681 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag 4741 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg 4801 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa 4861 gcgcgcaatt aaccctcact aaagggaaca aaagctgggt accgggcccc attattatca 4921 tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa aaaaaatcct 4981 tagctttcgc taaggatgat ttctggaatt cgcggccgca tctagag //