BBa_K535008 1 BBa_K535008 repC - ctRNA 2011-09-24T11:00:00Z 2015-05-08T01:12:36Z Low copy number plasmid pRmeGR4a from Sinorizobium meliloti GR4. The widespread replicons of repABC and repC families from -proteobacteria share high similarity in their replication initiator proteins (RepC). repC region belongs to a low copy-number plasmid pRmeGR4a from Sinorizobium meliloti GR4. This region contains only two genes: one encoding the initiator protein RepC (46.8 kDa) and other, an antisense RNA (67 nt). The constitutively expressed countertranscribed RNA (ctRNA) forms a single stem???loop structure that acts as an intrinsic rho-independent terminator. Transcriptional activity of ctRNA downregulate repC, both have sigma70-like promoters. Promoter of repC (T1) is relatively far from start site and is influenced by transcription of ctRNA which has promoter in reverse direction. Compatibility between plasmids is the ability to get two or more classes of plasmids at the same organism. The ctRNA is a strong trans-incompatibility factor and negative regulator of repC expression [1], this has been seen in other families of RepC class [2]. RepC is the minimal region required for stable replication of a member of the repC family, it works in Rhizobials . false false _701_ 0 8698 9 Not in stock false In order to have the same result as in pUX19-XKR5 [1] there are 290 bps upstream from start transcription site of repC. To get some repC downstream sequence there are added 51bp. false iGEM11_UNAM-Genomics_ Mexico annotation2139600 1 -35 box Promoter 1 range2139600 1 27 33 annotation2139602 1 -35 box Promoter 2 range2139602 1 224 229 annotation2139598 1 repC range2139598 1 292 1569 annotation2139601 1 -10 box Promoter 2 range2139601 1 201 209 annotation2139597 1 ctRNA range2139597 1 134 193 annotation2139599 1 -10 box Promoter 1 range2139599 1 51 57 BBa_K535008_sequence 1 tacaggcttttgcgtgtagcacgttgttgcacctttttggtttatcaggttagttttcaaatggcggaatcactcgtgattccggcgctcacgcattcttatcaaatttcacggagatgacaggccagaccagaaaagaaaaaccgctcccgaacaagtccggaagcgggggtcaaagagtagataaataagccgaatcctttctcgcagaccctcccttccctgtcaattgcaaaagcacctggtcggtgaacggcgaagctttgcctggtccctaaatggagacgaggaatggggaatacccaaattcatcagcggccaatcggccgcaggataacgccgcagcgtgcgcaattccggcgtttggcagagtcggcggatatcggaaccgtgacgcgtgcccaattggccgtgcttgcgcaaagcctgacatgcacagggctaatcaacgggaccgaaagccacctattggtagcgctgatcaatacggttcccgctgattcgttcgacaggtccggccgtctgatcgtattcaagtcgaaccagcaactggccttcgaaattggccggtcggtcggtcgcgtcagccgcatgctatccagcctcttcgacgctggcctgatcacgatgcaggacagcggcaactataagcgctatcccatccgcaatggcgacggcgagatcacggacggttgcgggatcgaccttcgcatcttgatcgtgcgatatgccgagcttgacgagctcgtgagacaggcaaggtctcagaagaaagccgccgacgcggcgctgcggcgctatcgtggggcgcttcgcaatgctcgctatgccctcgcggccgccgcgcgtctctcggaggattctttgagccgtctcggcgcccggatcgagcgggtgagcaacattgtcggaatcgccggcaaggcatcgagtcgcgtgctacggcgggcaacggctcttctggagtggttgatagagcggatgatgcagctcccgcgccgatcggcagaagcgcagaaaacgcaaaatatgacatgcccgtatgtcgaaaacggcatgcacaaacagattacaacccctcatccctttgacgatagtagtgaggaacggcgttcggcttacgccgaacaactcagttcgccaaaggctggctccgccagcaaatgggcttttgaagaaagcctgaggcggcgccggagcgaaatcaaccagccacggcgcttgcggcacgcaatggtggcattgcaagacgtgcttagggcggtgccagcgttgaagacctacgggtttgcccccaccacttgggccgacctggcgcgggcggtgccgctgatgtgccggtttgccgggatctccgaggatgcccgcgctcgcgccgtggagcagatgggcgagcagcaagccgccgtcgccgttgccgtgacacttgagaaatacgaccggcaggaagtcaactcgccgggaggatatttgcgggctatgacggaccgcgctagagcgggcgaattgcatctcgcccgctctgtcttcgggctcgctgcccgaaattccatggagatcagacattgagacgtaacgctttgctggtgcttttcgccgccctcgtcattgttcacggtt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z