BBa_K537000
1
atRS-ChZ
Atrazine Riboswitch-CheZ fusion
2011-07-18T11:00:00Z
2015-05-08T01:12:36Z
The CheZ gene was isolated from genomic DNA using PCR where the forward primers in the reaction contained the sequence for the atrazine riboswitch.
This DNA part will encode for an RNA riboswitch senstive to atrazine. The riboswitch, when no atrazine is present, prevents the exposure of the RBS. When atrazine is present, it binds to the riboswitch exposing the RBS and allows for the translation of the adjoining gene. In this case, the gene which will be expressed is CheZ, which a protein fundamental to bacterial movement.
false
false
_703_
0
9307
9
It's complicated
false
The effectiveness of the atrazine riboswitch is determined by the ability of the RNA 'loop' to hide the RBS preventing translation. The sequence of bases within the riboswitch will determine the formation of the loop and its leakiness.
false
Natasia Kruger
annotation2134325
1
CheZ
range2134325
1
86
727
annotation2134324
1
start
range2134324
1
86
88
annotation2134322
1
Atrazine riboswitch
range2134322
1
1
88
annotation2134323
1
C -> T
range2134323
1
55
55
BBa_K537000_sequence
1
gggacagggctagcatgaggcggggtaaaattgctccgataaaaacgcaaagtcttgcaggtcgacgccagggtggaacaacaagatgatgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagatacacgacaatttctggcagatgtacccgcgcataccagctttactaacgcgcaactgctggaaatcatgatggcgcaggattttcaggatctcaccgggcaggtcattaagcggatgatggatgtcattcaggagatcgaacgccagttgctgatggtgctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcgataccagcaaagccggtgtggtagccagtcaggatcaggtggacgatttgttggatagtcttggattt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z