BBa_K537002
1
thRS2-ChZ
Theophylline Riboswitch 2-CheZ
2011-07-18T11:00:00Z
2015-05-08T01:12:36Z
The CheZ gene was isolated from genomic DNA using PCR where the forward primers in the reaction contained the sequence for the theophylline riboswitch.
This DNA part will encode for an RNA riboswitch senstive to theophylline. The riboswitch, in the absence of theophylline, prevents the exposure of the RBS. When theophylline is present, it binds to the riboswitch exposing the RBS and allows for the translation of the adjoining gene. In this case, the gene which will be expressed is CheZ, which a protein fundamental to bacterial movement. This riboswitch has a stronger fold increase protein expression compared with Theophylline Riboswitch 1 + RBS + CheZ
false
false
_703_
0
9307
9
In stock
true
The effectiveness of the theophylline riboswitch is determined by the ability of the RNA 'loop' to hide the RBS preventing translation. The sequence of bases within the riboswitch will determine the formation of the loop and its leakiness.
false
Natasia Kruger
annotation2127176
1
rbs
range2127176
1
43
47
annotation2127178
1
Aptamer
range2127178
1
1
26
annotation2127175
1
start
range2127175
1
58
60
annotation2127179
1
CheZ
range2127179
1
61
702
annotation2127177
1
Theophylline riboswitch (type2)
range2127177
1
1
60
BBa_K537002_sequence
1
ggtgataccagcatcgtcttgatgcccttggcagcaccctgctaaggtaacaacaagatgatgatgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagatacacgacaatttctggcagatgtacccgcgcataccagctttactaacgcgcaactgctggaaatcatgatggcgcaggattttcaggatctcaccgggcaggtcattaagcggatgatggatgtcattcaggagatcgaacgccagttgctgatggtgctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcgataccagcaaagccggtgtggtagccagtcaggatcaggtggacgatttgttggatagtcttggattt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z