LOCUS       BBa_K537009              959 bp    DNA     linear       01-Jun-2024
DEFINITION  Promoter-Theophylline riboswitch 1-Venus-Double terminator
ACCESSION   BBa_K537009
VERSION     BBa_K537009.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    100..102
                     /label=start
     RBS             90..92
                     /label=rbs
     misc_feature    44..102
                     /label=Theophylline riboswitch (type 1)
     misc_feature    44..69
                     /label=Aptamer
     CDS             100..822
                     /label=Venus
     misc_feature    44..822
                     /label=BBa_K537003
     stem_loop       842..885
                     /label=stem_loop
     misc_feature    831..910
                     /label=BBa_B0010
     terminator      831..910
                     /label=BBa_B0010
     stem_loop       926..945
                     /label=T7 TE
     misc_feature    952..952
                     /label=stop
     polyA_site      946..959
                     /label=polya
     misc_feature    919..959
                     /label=BBa_B0012
     terminator      919..959
                     /label=BBa_B0012
     terminator      831..959
                     /label=BBa_B0015
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagggtgata ccagcatcgt
       61 cttgatgccc ttggcagcac cccgctgcaa gacaacaaga tggtttcaaa gggtgaagaa
      121 ttatttactg gtgttgttcc aattttagtt gaattagatg gtgatgttaa cggtcacaag
      181 ttttcagttt caggtgaagg tgaaggtgat gctacttacg gtaagttaac tttaaagtta
      241 atttgtacta ctggtaagtt accagttcca tggccaactt tagttactac tttaggttac
      301 ggtttacaat gttttgctcg ttacccagat cacatgaagc aacacgattt ttttaagtca
      361 gctatgccag aaggttacgt tcaagaacgt actatttttt ttaaggatga tggtaactac
      421 aagactcgtg ctgaagttaa gtttgaaggt gatactttag ttaaccgtat tgaattaaag
      481 ggtattgatt ttaaggaaga tggtaacatt ttaggtcaca agttagaata caactacaac
      541 tcacacaacg tttacattac tgctgataag caaaagaacg gtattaaggc taactttaag
      601 attcgtcaca acattgaaga tggtggtgtt caattagctg atcactacca acaaaacact
      661 ccaattggtg atggtccagt tttattacca gataaccact acttatcata ccaatcaaag
      721 ttatcaaagg atccaaacga aaagcgtgat cacatggttt tattagaatt tgttactgct
      781 gctggtatta ctttaggtat ggatgaatta tacaagtaat aatactagag ccaggcatca
      841 aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt
      901 gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//