BBa_K537009
1
BBa_K537009
Promoter-Theophylline riboswitch 1-Venus-Double terminator
2011-09-15T11:00:00Z
2015-05-08T01:12:36Z
Coming soon
Coming soon
false
false
_703_
0
9345
9
It's complicated
true
Coming soon
false
Ezio Fok
component2220762
1
BBa_K537003
component2220756
1
BBa_J23119
component2220769
1
BBa_B0015
annotation2220769
1
BBa_B0015
range2220769
1
831
959
annotation2220756
1
BBa_J23119
range2220756
1
1
35
annotation2220762
1
BBa_K537003
range2220762
1
44
822
BBa_K537003
1
thRS1-V
Theophylline Riboswitch 1-Venus
2011-09-08T11:00:00Z
2015-05-08T01:12:36Z
Topp and Gallivan JACS 2007also BBa_K249026 and BBa_K411001 fusion with Venus reporter BBa_K354002
This is a theohylline riboswitch clone 8.1 (Topp and Gallivan JACS 2007) also BBa_K249026 and BBa_K411001 fusion with Venus reporter BBa_K354002. This part regulates the expression of venus in a theophylline-dependent fashion through an RNA aptmer specific to theophylline.
false
false
_703_
0
9308
9
In stock
true
none
false
Gloria Hlongwane
annotation2127181
1
rbs
range2127181
1
47
49
annotation2127182
1
Theophylline riboswitch (type 1)
range2127182
1
1
59
annotation2127184
1
Venus
range2127184
1
57
779
annotation2127183
1
Aptamer
range2127183
1
1
26
annotation2127180
1
start
range2127180
1
57
59
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1686
1
T7 TE
range1686
1
8
27
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916610
1
BBa_B0010
range1916610
1
1
80
annotation1916612
1
BBa_B0012
range1916612
1
89
129
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K537009_sequence
1
ttgacagctagctcagtcctaggtataatgctagctactagagggtgataccagcatcgtcttgatgcccttggcagcaccccgctgcaagacaacaagatggtttcaaagggtgaagaattatttactggtgttgttccaattttagttgaattagatggtgatgttaacggtcacaagttttcagtttcaggtgaaggtgaaggtgatgctacttacggtaagttaactttaaagttaatttgtactactggtaagttaccagttccatggccaactttagttactactttaggttacggtttacaatgttttgctcgttacccagatcacatgaagcaacacgatttttttaagtcagctatgccagaaggttacgttcaagaacgtactattttttttaaggatgatggtaactacaagactcgtgctgaagttaagtttgaaggtgatactttagttaaccgtattgaattaaagggtattgattttaaggaagatggtaacattttaggtcacaagttagaatacaactacaactcacacaacgtttacattactgctgataagcaaaagaacggtattaaggctaactttaagattcgtcacaacattgaagatggtggtgttcaattagctgatcactaccaacaaaacactccaattggtgatggtccagttttattaccagataaccactacttatcataccaatcaaagttatcaaaggatccaaacgaaaagcgtgatcacatggttttattagaatttgttactgctgctggtattactttaggtatggatgaattatacaagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K537003_sequence
1
ggtgataccagcatcgtcttgatgcccttggcagcaccccgctgcaagacaacaagatggtttcaaagggtgaagaattatttactggtgttgttccaattttagttgaattagatggtgatgttaacggtcacaagttttcagtttcaggtgaaggtgaaggtgatgctacttacggtaagttaactttaaagttaatttgtactactggtaagttaccagttccatggccaactttagttactactttaggttacggtttacaatgttttgctcgttacccagatcacatgaagcaacacgatttttttaagtcagctatgccagaaggttacgttcaagaacgtactattttttttaaggatgatggtaactacaagactcgtgctgaagttaagtttgaaggtgatactttagttaaccgtattgaattaaagggtattgattttaaggaagatggtaacattttaggtcacaagttagaatacaactacaactcacacaacgtttacattactgctgataagcaaaagaacggtattaaggctaactttaagattcgtcacaacattgaagatggtggtgttcaattagctgatcactaccaacaaaacactccaattggtgatggtccagttttattaccagataaccactacttatcataccaatcaaagttatcaaaggatccaaacgaaaagcgtgatcacatggttttattagaatttgttactgctgctggtattactttaggtatggatgaattatacaagtaataa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z