LOCUS       BBa_K537011             1583 bp    DNA     linear       16-Feb-2025
DEFINITION  Promoter-Theophylline riboswitch 1-CheZ-Venus-Double terminator
ACCESSION   BBa_K537011
VERSION     BBa_K537011.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    36..61
                     /label=Aptamer
     misc_feature    36..94
                     /label=Theophylline riboswitch (type 1)
     RBS             82..84
                     /label=rbs
     CDS             92..733
                     /label=CheZ
     misc_feature    92..94
                     /label=start
     misc_feature    36..733
                     /label=BBa_K537001
     CDS             734..1454
                     /label=Venus
     misc_feature    734..1454
                     /label=BBa_K537006
     stem_loop       1466..1509
                     /label=stem_loop
     misc_feature    1455..1534
                     /label=BBa_B0010
     terminator      1455..1534
                     /label=BBa_B0010
     stem_loop       1550..1569
                     /label=T7 TE
     misc_feature    1576..1576
                     /label=stop
     polyA_site      1570..1583
                     /label=polya
     misc_feature    1543..1583
                     /label=BBa_B0012
     terminator      1543..1583
                     /label=BBa_B0012
     terminator      1455..1583
                     /label=BBa_B0015
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagcggtga taccagcatc gtcttgatgc
       61 ccttggcagc accccgctgc aagacaacaa gatgatgcaa ccatcaatca aacctgctga
      121 cgagcattca gctggcgata tcattgcgcg catcggcagc ctgacgcgta tgctgcgcga
      181 cagtttgcgg gaactggggc tggatcaggc cattgccgaa gcggcggaag ccatccccga
      241 tgcgcgcgat cgtttgtact atgttgtgca gatgaccgcc caggctgcgg agcgggcgct
      301 gaacagtgtt gaggcgtcac aaccgcatca ggatcaaatg gagaaatcag caaaagcgtt
      361 aacccaacgt tgggatgact ggtttgccga tccgattgac cttgccgacg cccgtgaact
      421 ggtaacagat acacgacaat ttctggcaga tgtacccgcg cataccagct ttactaacgc
      481 gcaactgctg gaaatcatga tggcgcagga ttttcaggat ctcaccgggc aggtcattaa
      541 gcggatgatg gatgtcattc aggagatcga acgccagttg ctgatggtgc tgttggaaaa
      601 catcccggaa caggagtcgc gtccaaaacg tgaaaaccag agtttgctta atggacctca
      661 ggtcgatacc agcaaagccg gtgtggtagc cagtcaggat caggtggacg atttgttgga
      721 tagtcttgga tttgtttcaa agggtgaaga attatttact ggtgttgttc caattttagt
      781 tgaattagat ggtgatgtta acggtcacaa gttttcagtt tcaggtgaag gtgaaggtga
      841 tgctacttac ggtaagttaa ctttaaagtt aatttgtact actggtaagt taccagttcc
      901 atggccaact ttagttacta ctttaggtta cggtttacaa tgttttgctc gttacccaga
      961 tcacatgaag caacacgatt tttttaagtc agctatgcca gaaggttacg ttcaagaacg
     1021 tactattttt tttaaggatg atggtaacta caagactcgt gctgaagtta agtttgaagg
     1081 tgatacttta gttaaccgta ttgaattaaa gggtattgat tttaaggaag atggtaacat
     1141 tttaggtcac aagttagaat acaactacaa ctcacacaac gtttacatta ctgctgataa
     1201 gcaaaagaac ggtattaagg ctaactttaa gattcgtcac aacattgaag atggtggtgt
     1261 tcaattagct gatcactacc aacaaaacac tccaattggt gatggtccag ttttattacc
     1321 agataaccac tacttatcat accaatcaaa gttatcaaag gatccaaacg aaaagcgtga
     1381 tcacatggtt ttattagaat ttgttactgc tgctggtatt actttaggta tggatgaatt
     1441 atacaagtaa taatccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
     1501 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
     1561 gggtgggcct ttctgcgttt ata
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