LOCUS       BBa_K537012             1587 bp    DNA     linear       22-Dec-2024
DEFINITION  Promoter-Theophylline riboswitch 2-CheZ-Venus-Double terminator
ACCESSION   BBa_K537012
VERSION     BBa_K537012.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     misc_feature    93..95
                     /label=start
     RBS             78..82
                     /label=rbs
     misc_feature    36..95
                     /label=Theophylline riboswitch (type2)
     misc_feature    36..61
                     /label=Aptamer
     CDS             96..737
                     /label=CheZ
     misc_feature    36..737
                     /label=BBa_K537002
     CDS             738..1458
                     /label=Venus
     misc_feature    738..1458
                     /label=BBa_K537006
     stem_loop       1470..1513
                     /label=stem_loop
     misc_feature    1459..1538
                     /label=BBa_B0010
     terminator      1459..1538
                     /label=BBa_B0010
     stem_loop       1554..1573
                     /label=T7 TE
     misc_feature    1580..1580
                     /label=stop
     polyA_site      1574..1587
                     /label=polya
     misc_feature    1547..1587
                     /label=BBa_B0012
     terminator      1547..1587
                     /label=BBa_B0012
     terminator      1459..1587
                     /label=BBa_B0015
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagcggtga taccagcatc gtcttgatgc
       61 ccttggcagc accctgctaa ggtaacaaca agatgatgat gcaaccatca atcaaacctg
      121 ctgacgagca ttcagctggc gatatcattg cgcgcatcgg cagcctgacg cgtatgctgc
      181 gcgacagttt gcgggaactg gggctggatc aggccattgc cgaagcggcg gaagccatcc
      241 ccgatgcgcg cgatcgtttg tactatgttg tgcagatgac cgcccaggct gcggagcggg
      301 cgctgaacag tgttgaggcg tcacaaccgc atcaggatca aatggagaaa tcagcaaaag
      361 cgttaaccca acgttgggat gactggtttg ccgatccgat tgaccttgcc gacgcccgtg
      421 aactggtaac agatacacga caatttctgg cagatgtacc cgcgcatacc agctttacta
      481 acgcgcaact gctggaaatc atgatggcgc aggattttca ggatctcacc gggcaggtca
      541 ttaagcggat gatggatgtc attcaggaga tcgaacgcca gttgctgatg gtgctgttgg
      601 aaaacatccc ggaacaggag tcgcgtccaa aacgtgaaaa ccagagtttg cttaatggac
      661 ctcaggtcga taccagcaaa gccggtgtgg tagccagtca ggatcaggtg gacgatttgt
      721 tggatagtct tggatttgtt tcaaagggtg aagaattatt tactggtgtt gttccaattt
      781 tagttgaatt agatggtgat gttaacggtc acaagttttc agtttcaggt gaaggtgaag
      841 gtgatgctac ttacggtaag ttaactttaa agttaatttg tactactggt aagttaccag
      901 ttccatggcc aactttagtt actactttag gttacggttt acaatgtttt gctcgttacc
      961 cagatcacat gaagcaacac gattttttta agtcagctat gccagaaggt tacgttcaag
     1021 aacgtactat tttttttaag gatgatggta actacaagac tcgtgctgaa gttaagtttg
     1081 aaggtgatac tttagttaac cgtattgaat taaagggtat tgattttaag gaagatggta
     1141 acattttagg tcacaagtta gaatacaact acaactcaca caacgtttac attactgctg
     1201 ataagcaaaa gaacggtatt aaggctaact ttaagattcg tcacaacatt gaagatggtg
     1261 gtgttcaatt agctgatcac taccaacaaa acactccaat tggtgatggt ccagttttat
     1321 taccagataa ccactactta tcataccaat caaagttatc aaaggatcca aacgaaaagc
     1381 gtgatcacat ggttttatta gaatttgtta ctgctgctgg tattacttta ggtatggatg
     1441 aattatacaa gtaataatcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
     1501 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
     1561 cttcgggtgg gcctttctgc gtttata
//