BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K537006 1 Venus C Venus C-Fusion 2011-09-08T11:00:00Z 2015-05-08T01:12:36Z Under construction Under construction false false _703_ 0 9308 9 In stock true Under construction false Gloria Hlongwane annotation2133606 1 Venus range2133606 1 1 721 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_J23119 1 BBa_J23119 constitutive promoter family member 2006-08-23T11:00:00Z 2015-08-31T04:08:40Z Overlap extension of synthetic oligonucleotides Released HQ 2013 Later false true _52_ 0 483 95 In stock false N/A true John Anderson BBa_K537002 1 thRS2-ChZ Theophylline Riboswitch 2-CheZ 2011-07-18T11:00:00Z 2015-05-08T01:12:36Z The CheZ gene was isolated from genomic DNA using PCR where the forward primers in the reaction contained the sequence for the theophylline riboswitch. This DNA part will encode for an RNA riboswitch senstive to theophylline. The riboswitch, in the absence of theophylline, prevents the exposure of the RBS. When theophylline is present, it binds to the riboswitch exposing the RBS and allows for the translation of the adjoining gene. In this case, the gene which will be expressed is CheZ, which a protein fundamental to bacterial movement. This riboswitch has a stronger fold increase protein expression compared with Theophylline Riboswitch 1 + RBS + CheZ false false _703_ 0 9307 9 In stock true The effectiveness of the theophylline riboswitch is determined by the ability of the RNA 'loop' to hide the RBS preventing translation. The sequence of bases within the riboswitch will determine the formation of the loop and its leakiness. false Natasia Kruger annotation2127177 1 Theophylline riboswitch (type2) range2127177 1 1 60 annotation2127176 1 rbs range2127176 1 43 47 annotation2127178 1 Aptamer range2127178 1 1 26 annotation2127175 1 start range2127175 1 58 60 annotation2127179 1 CheZ range2127179 1 61 702 BBa_K537012 1 BBa_K537012 Promoter-Theophylline riboswitch 2-CheZ-Venus-Double terminator 2011-09-15T11:00:00Z 2015-05-08T01:12:36Z Coming soon Coming soon false false _703_ 0 9345 9 Not in stock true Coming soon false Ezio Fok component2133659 1 BBa_J23119 component2133665 1 BBa_K537002 component2133674 1 BBa_B0015 component2133667 1 BBa_K537006 annotation2133674 1 BBa_B0015 range2133674 1 1459 1587 annotation2133665 1 BBa_K537002 range2133665 1 36 737 annotation2133667 1 BBa_K537006 range2133667 1 738 1458 annotation2133659 1 BBa_J23119 range2133659 1 1 35 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K537012_sequence 1 ttgacagctagctcagtcctaggtataatgctagcggtgataccagcatcgtcttgatgcccttggcagcaccctgctaaggtaacaacaagatgatgatgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagatacacgacaatttctggcagatgtacccgcgcataccagctttactaacgcgcaactgctggaaatcatgatggcgcaggattttcaggatctcaccgggcaggtcattaagcggatgatggatgtcattcaggagatcgaacgccagttgctgatggtgctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcgataccagcaaagccggtgtggtagccagtcaggatcaggtggacgatttgttggatagtcttggatttgtttcaaagggtgaagaattatttactggtgttgttccaattttagttgaattagatggtgatgttaacggtcacaagttttcagtttcaggtgaaggtgaaggtgatgctacttacggtaagttaactttaaagttaatttgtactactggtaagttaccagttccatggccaactttagttactactttaggttacggtttacaatgttttgctcgttacccagatcacatgaagcaacacgatttttttaagtcagctatgccagaaggttacgttcaagaacgtactattttttttaaggatgatggtaactacaagactcgtgctgaagttaagtttgaaggtgatactttagttaaccgtattgaattaaagggtattgattttaaggaagatggtaacattttaggtcacaagttagaatacaactacaactcacacaacgtttacattactgctgataagcaaaagaacggtattaaggctaactttaagattcgtcacaacattgaagatggtggtgttcaattagctgatcactaccaacaaaacactccaattggtgatggtccagttttattaccagataaccactacttatcataccaatcaaagttatcaaaggatccaaacgaaaagcgtgatcacatggttttattagaatttgttactgctgctggtattactttaggtatggatgaattatacaagtaataatccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K537002_sequence 1 ggtgataccagcatcgtcttgatgcccttggcagcaccctgctaaggtaacaacaagatgatgatgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagatacacgacaatttctggcagatgtacccgcgcataccagctttactaacgcgcaactgctggaaatcatgatggcgcaggattttcaggatctcaccgggcaggtcattaagcggatgatggatgtcattcaggagatcgaacgccagttgctgatggtgctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcgataccagcaaagccggtgtggtagccagtcaggatcaggtggacgatttgttggatagtcttggattt BBa_K537006_sequence 1 gtttcaaagggtgaagaattatttactggtgttgttccaattttagttgaattagatggtgatgttaacggtcacaagttttcagtttcaggtgaaggtgaaggtgatgctacttacggtaagttaactttaaagttaatttgtactactggtaagttaccagttccatggccaactttagttactactttaggttacggtttacaatgttttgctcgttacccagatcacatgaagcaacacgatttttttaagtcagctatgccagaaggttacgttcaagaacgtactattttttttaaggatgatggtaactacaagactcgtgctgaagttaagtttgaaggtgatactttagttaaccgtattgaattaaagggtattgattttaaggaagatggtaacattttaggtcacaagttagaatacaactacaactcacacaacgtttacattactgctgataagcaaaagaacggtattaaggctaactttaagattcgtcacaacattgaagatggtggtgttcaattagctgatcactaccaacaaaacactccaattggtgatggtccagttttattaccagataaccactacttatcataccaatcaaagttatcaaaggatccaaacgaaaagcgtgatcacatggttttattagaatttgttactgctgctggtattactttaggtatggatgaattatacaagtaataat BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J23119_sequence 1 ttgacagctagctcagtcctaggtataatgctagc BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z