LOCUS       BBa_K537013             1567 bp    DNA     linear       26-Dec-2024
DEFINITION  Promoter-RBS-CheZ-Venus-Double terminator
ACCESSION   BBa_K537013
VERSION     BBa_K537013.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     RBS             45..49
                     /label=rbs
     CDS             60..701
                     /label=CheZ
     CDS             44..701
                     /label=BBa_K537007
     CDS             710..1430
                     /label=Venus
     misc_feature    710..1430
                     /label=BBa_K537006
     stem_loop       1450..1493
                     /label=stem_loop
     misc_feature    1439..1518
                     /label=BBa_B0010
     terminator      1439..1518
                     /label=BBa_B0010
     stem_loop       1534..1553
                     /label=T7 TE
     misc_feature    1560..1560
                     /label=stop
     polyA_site      1554..1567
                     /label=polya
     misc_feature    1527..1567
                     /label=BBa_B0012
     terminator      1527..1567
                     /label=BBa_B0012
     terminator      1439..1567
                     /label=BBa_B0015
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagctaaggt aacatcatga
       61 tgatgcaacc atcaatcaaa cctgctgacg agcattcagc tggcgatatc attgcgcgca
      121 tcggcagcct gacgcgtatg ctgcgcgaca gtttgcggga actggggctg gatcaggcca
      181 ttgccgaagc ggcggaagcc atccccgatg cgcgcgatcg tttgtactat gttgtgcaga
      241 tgaccgccca ggctgcggag cgggcgctga acagtgttga ggcgtcacaa ccgcatcagg
      301 atcaaatgga gaaatcagca aaagcgttaa cccaacgttg ggatgactgg tttgccgatc
      361 cgattgacct tgccgacgcc cgtgaactgg taacagatac acgacaattt ctggcagatg
      421 tacccgcgca taccagcttt actaacgcgc aactgctgga aatcatgatg gcgcaggatt
      481 ttcaggatct caccgggcag gtcattaagc ggatgatgga tgtcattcag gagatcgaac
      541 gccagttgct gatggtgctg ttggaaaaca tcccggaaca ggagtcgcgt ccaaaacgtg
      601 aaaaccagag tttgcttaat ggacctcagg tcgataccag caaagccggt gtggtagcca
      661 gtcaggatca ggtggacgat ttgttggata gtcttggatt ttactagagg tttcaaaggg
      721 tgaagaatta tttactggtg ttgttccaat tttagttgaa ttagatggtg atgttaacgg
      781 tcacaagttt tcagtttcag gtgaaggtga aggtgatgct acttacggta agttaacttt
      841 aaagttaatt tgtactactg gtaagttacc agttccatgg ccaactttag ttactacttt
      901 aggttacggt ttacaatgtt ttgctcgtta cccagatcac atgaagcaac acgatttttt
      961 taagtcagct atgccagaag gttacgttca agaacgtact atttttttta aggatgatgg
     1021 taactacaag actcgtgctg aagttaagtt tgaaggtgat actttagtta accgtattga
     1081 attaaagggt attgatttta aggaagatgg taacatttta ggtcacaagt tagaatacaa
     1141 ctacaactca cacaacgttt acattactgc tgataagcaa aagaacggta ttaaggctaa
     1201 ctttaagatt cgtcacaaca ttgaagatgg tggtgttcaa ttagctgatc actaccaaca
     1261 aaacactcca attggtgatg gtccagtttt attaccagat aaccactact tatcatacca
     1321 atcaaagtta tcaaaggatc caaacgaaaa gcgtgatcac atggttttat tagaatttgt
     1381 tactgctgct ggtattactt taggtatgga tgaattatac aagtaataat tactagagcc
     1441 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     1501 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     1561 gtttata
//