BBa_K537013 1 BBa_K537013 Promoter-RBS-CheZ-Venus-Double terminator 2011-09-15T11:00:00Z 2015-05-08T01:12:36Z Coming soon Coming soon false false _703_ 0 9345 9 Not in stock false Coming soon false Ezio Fok component2134419 1 BBa_B0015 component2134407 1 BBa_J23119 component2134410 1 BBa_K537007 component2134412 1 BBa_K537006 annotation2134407 1 BBa_J23119 range2134407 1 1 35 annotation2134419 1 BBa_B0015 range2134419 1 1439 1567 annotation2134412 1 BBa_K537006 range2134412 1 710 1430 annotation2134410 1 BBa_K537007 range2134410 1 44 701 BBa_K537007 1 ChZ N-fusi RBS CheZ N-Fusion 2011-09-11T11:00:00Z 2015-05-08T01:12:36Z PCR primers The RBS CheZ- Fusion BioBrick consists of a RBS fused to the E. coli CheZ motility factor that lacks a stop codon at the N-terminus. CheZ in this part contains the standard BioBrick prefix and the Freiburg (2007) fusion suffix. Using this BioBrick CheZ is constitutively expressed. false false _703_ 0 9345 9 It's complicated false None false Ezio Fok annotation2127322 1 rbs range2127322 1 2 6 annotation2127323 1 CheZ range2127323 1 17 658 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_K537006 1 Venus C Venus C-Fusion 2011-09-08T11:00:00Z 2015-05-08T01:12:36Z Under construction Under construction false false _703_ 0 9308 9 In stock true Under construction false Gloria Hlongwane annotation2133606 1 Venus range2133606 1 1 721 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916610 1 BBa_B0010 range1916610 1 1 80 annotation1916612 1 BBa_B0012 range1916612 1 89 129 BBa_J23119 1 BBa_J23119 constitutive promoter family member 2006-08-23T11:00:00Z 2015-08-31T04:08:40Z Overlap extension of synthetic oligonucleotides Released HQ 2013 Later false true _52_ 0 483 95 In stock false N/A true John Anderson BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K537013_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactagagctaaggtaacatcatgatgatgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagatacacgacaatttctggcagatgtacccgcgcataccagctttactaacgcgcaactgctggaaatcatgatggcgcaggattttcaggatctcaccgggcaggtcattaagcggatgatggatgtcattcaggagatcgaacgccagttgctgatggtgctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcgataccagcaaagccggtgtggtagccagtcaggatcaggtggacgatttgttggatagtcttggattttactagaggtttcaaagggtgaagaattatttactggtgttgttccaattttagttgaattagatggtgatgttaacggtcacaagttttcagtttcaggtgaaggtgaaggtgatgctacttacggtaagttaactttaaagttaatttgtactactggtaagttaccagttccatggccaactttagttactactttaggttacggtttacaatgttttgctcgttacccagatcacatgaagcaacacgatttttttaagtcagctatgccagaaggttacgttcaagaacgtactattttttttaaggatgatggtaactacaagactcgtgctgaagttaagtttgaaggtgatactttagttaaccgtattgaattaaagggtattgattttaaggaagatggtaacattttaggtcacaagttagaatacaactacaactcacacaacgtttacattactgctgataagcaaaagaacggtattaaggctaactttaagattcgtcacaacattgaagatggtggtgttcaattagctgatcactaccaacaaaacactccaattggtgatggtccagttttattaccagataaccactacttatcataccaatcaaagttatcaaaggatccaaacgaaaagcgtgatcacatggttttattagaatttgttactgctgctggtattactttaggtatggatgaattatacaagtaataattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K537006_sequence 1 gtttcaaagggtgaagaattatttactggtgttgttccaattttagttgaattagatggtgatgttaacggtcacaagttttcagtttcaggtgaaggtgaaggtgatgctacttacggtaagttaactttaaagttaatttgtactactggtaagttaccagttccatggccaactttagttactactttaggttacggtttacaatgttttgctcgttacccagatcacatgaagcaacacgatttttttaagtcagctatgccagaaggttacgttcaagaacgtactattttttttaaggatgatggtaactacaagactcgtgctgaagttaagtttgaaggtgatactttagttaaccgtattgaattaaagggtattgattttaaggaagatggtaacattttaggtcacaagttagaatacaactacaactcacacaacgtttacattactgctgataagcaaaagaacggtattaaggctaactttaagattcgtcacaacattgaagatggtggtgttcaattagctgatcactaccaacaaaacactccaattggtgatggtccagttttattaccagataaccactacttatcataccaatcaaagttatcaaaggatccaaacgaaaagcgtgatcacatggttttattagaatttgttactgctgctggtattactttaggtatggatgaattatacaagtaataat BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J23119_sequence 1 ttgacagctagctcagtcctaggtataatgctagc BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K537007_sequence 1 ctaaggtaacatcatgatgatgcaaccatcaatcaaacctgctgacgagcattcagctggcgatatcattgcgcgcatcggcagcctgacgcgtatgctgcgcgacagtttgcgggaactggggctggatcaggccattgccgaagcggcggaagccatccccgatgcgcgcgatcgtttgtactatgttgtgcagatgaccgcccaggctgcggagcgggcgctgaacagtgttgaggcgtcacaaccgcatcaggatcaaatggagaaatcagcaaaagcgttaacccaacgttgggatgactggtttgccgatccgattgaccttgccgacgcccgtgaactggtaacagatacacgacaatttctggcagatgtacccgcgcataccagctttactaacgcgcaactgctggaaatcatgatggcgcaggattttcaggatctcaccgggcaggtcattaagcggatgatggatgtcattcaggagatcgaacgccagttgctgatggtgctgttggaaaacatcccggaacaggagtcgcgtccaaaacgtgaaaaccagagtttgcttaatggacctcaggtcgataccagcaaagccggtgtggtagccagtcaggatcaggtggacgatttgttggatagtcttggattt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z