LOCUS       BBa_K537016             1501 bp    DNA     linear       07-Dec-2025
DEFINITION  Promoter-RBS-CheZ-mCherry-Double terminator
ACCESSION   BBa_K537016
VERSION     BBa_K537016.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23119
     RBS             37..41
                     /label=rbs
     CDS             52..693
                     /label=CheZ
     CDS             36..693
                     /label=BBa_K537007
     CDS             694..1372
                     /label=mRFP
     misc_feature    694..1372
                     /label=BBa_K537005
     stem_loop       1384..1427
                     /label=stem_loop
     misc_feature    1373..1452
                     /label=BBa_B0010
     terminator      1373..1452
                     /label=BBa_B0010
     stem_loop       1468..1487
                     /label=T7 TE
     misc_feature    1494..1494
                     /label=stop
     polyA_site      1488..1501
                     /label=polya
     misc_feature    1461..1501
                     /label=BBa_B0012
     terminator      1461..1501
                     /label=BBa_B0012
     terminator      1373..1501
                     /label=BBa_B0015
ORIGIN
        1 ttgacagcta gctcagtcct aggtataatg ctagcctaag gtaacatcat gatgatgcaa
       61 ccatcaatca aacctgctga cgagcattca gctggcgata tcattgcgcg catcggcagc
      121 ctgacgcgta tgctgcgcga cagtttgcgg gaactggggc tggatcaggc cattgccgaa
      181 gcggcggaag ccatccccga tgcgcgcgat cgtttgtact atgttgtgca gatgaccgcc
      241 caggctgcgg agcgggcgct gaacagtgtt gaggcgtcac aaccgcatca ggatcaaatg
      301 gagaaatcag caaaagcgtt aacccaacgt tgggatgact ggtttgccga tccgattgac
      361 cttgccgacg cccgtgaact ggtaacagat acacgacaat ttctggcaga tgtacccgcg
      421 cataccagct ttactaacgc gcaactgctg gaaatcatga tggcgcagga ttttcaggat
      481 ctcaccgggc aggtcattaa gcggatgatg gatgtcattc aggagatcga acgccagttg
      541 ctgatggtgc tgttggaaaa catcccggaa caggagtcgc gtccaaaacg tgaaaaccag
      601 agtttgctta atggacctca ggtcgatacc agcaaagccg gtgtggtagc cagtcaggat
      661 caggtggacg atttgttgga tagtcttgga tttgcttcct ccgaagacgt tatcaaagag
      721 ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa
      781 ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa
      841 ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa
      901 gcttacgtta aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt
      961 ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac
     1021 tcctccctgc aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg
     1081 tccgacggtc cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg
     1141 tacccggaag acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt
     1201 ggtcactacg acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg
     1261 ggtgcttaca aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc
     1321 gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata atccaggcat
     1381 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1441 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     1501 a
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