##gff-version 3
##sequence-region BBa_K538002 1 1920
BBa_K538002	.	start_codon	1	3	.	+	0	ID=annotation2124233;Name=Start Codon
BBa_K538002	.	point_mutation	345	345	.	+	0	ID=annotation2127787;Name=A > G (Recode for PvuII site)
BBa_K538002	.	point_mutation	726	726	.	+	0	ID=annotation2127789;Name=A > G (Recode for EcoRI site)
BBa_K538002	.	point_mutation	945	945	.	+	0	ID=annotation2127790;Name=T > G (Recode for XbaI site)
BBa_K538002	.	point_mutation	1281	1281	.	+	0	ID=annotation2127791;Name=A > G (Recode for PvuII site)
BBa_K538002	.	point_mutation	1671	1671	.	+	0	ID=annotation2127792;Name=T > G (Recode for NheI site)
BBa_K538002	.	stop_codon	1915	1920	.	+	0	ID=annotation2124234;Name=Double Stop Codon
>BBa_K538002
atgggcaaaattattggtatcgatttaggcacaacaaactcgtgtgtagcagtccttgatggcggcaaagcacgcgtaa
ttgaaaacgcagagggtgatcgtacaaccccatcaattatcgcttataccgatgatgaaattattgtaggccaaccagc
aaagcgtcaagctgtaactaacccaacaaacacattctttgccattaagcgtttaatcggccgtcgttttaaagatgac
gaagtacaacgtgacgtgaacatcatgccattcaaaattatcggagctgataatggtgatgcatgggttgaatcacgtg
gtaataaaatggcaccaccacaagtttcggctgaaatcttgaaaaagatgaaaaagactgctgaagactttttaggtga
agaagtgactgaagcggttattaccgttcctgcttactttaacgattcacaacgtcaagcaacgaaagatgctggtcgt
atcgcaggtcttgatgttaagcgtattatcaacgaacctactgctgctgcacttgcatacggtatcgataagaagcaag
gcgacaacattgttgctgtatacgatttaggtggtggtacattcgatatttctatcatcgaaatcgacagtaacgatgg
cgaccaaacatttgaagtactagcaaccaatggtgatactcacttaggtggtgaagactttgataaccgtatgattaac
tatttagctgatgagttcaaaaaagaccagggcttagatcttcgtagagatcctttagcaatgcaacgtttgaaagaag
cggctgaaaaagcaaaaatcgagctttcaagcactaaccacactgaagttaacttgccttacatcactgctgatgcatc
aggtcctaagcatttagtggttaaaattactcgtgctaagttagagtcattagttgaagatttaattcaacgtacgcta
gagccgcttaaagttgcactagctgatgctgatttatcaatatctgatatcaatgaagtgattcttgttggtggccaga
ctcgtatgcctaaagtacaagaagcagtaactaacttcttcggcaaagagcctcgtaaagatgttaaccctgatgaagc
ggttgcagttggcgcggcgattcaggctggcgtactttctggtgaagtgaaagacgtacttctacttgacgttacacca
ctatctcttggtattgaaaccatgggcagtgtgatgaccaagcttatcgagaagaacaccactatcccgactaaagctc
agcaagtattctcaacggctgacgacaaccaaagtgcagtgacaattcacgtacttcaaggtgagcgtaagcaagctag
tgccaacaagtcattaggtcaatttaacctagaaggtattgagccagcaccacgtggccaaccacaggttgaagtgatg
ttcgacattgatgctgatggtatcttacatgtgtctgcaacagacaagaaaacaggtaagaaacaaaatattactatca
aagcttcttcaggtttgtctgatgaagaagttgaacaaatggtacgtgatgcagaagctcatgctgatgaagatgcgaa
atttgaagagttagttcaagcgcgtaatcaagcagatggtttagctcattcaactaaaaaacaagttgaagaagctggc
gatgcactagcgagcgacgaaaaagaaaagattgaagcggcaattgcaactttagaaactgccataaaaggcaaagata
aagaagctattgatacagctactcaagcgttaatcgaagcgtctgctaagttaatggaaattgctcaagctaaagctca
aggtgaagcagaaggtcaagcacacgatgctggccaagaaaagcctgctgatgatgttgttgatgctgagttcgaagaa
gttaaagacgacaaaaagtaataa