LOCUS BBa_K538102 1938 bp DNA linear 05-Nov-2024 DEFINITION RBS + SheDnaK ACCESSION BBa_K538102 VERSION BBa_K538102.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 misc_feature 19..21 /label=Start Codon misc_feature 1933..1938 /label=Double Stop Codon misc_feature 363..363 /label=A > G (Recode for PvuII site) misc_feature 744..744 /label=A > G (Recode for EcoRI site) misc_feature 963..963 /label=T > G (Recode for XbaI site) misc_feature 1299..1299 /label=A > G (Recode for PvuII site) misc_feature 1689..1689 /label=T > G (Recode for NheI site) CDS 19..1938 /label=BBa_K538002 ORIGIN 1 aaagaggaga aatactagat gggcaaaatt attggtatcg atttaggcac aacaaactcg 61 tgtgtagcag tccttgatgg cggcaaagca cgcgtaattg aaaacgcaga gggtgatcgt 121 acaaccccat caattatcgc ttataccgat gatgaaatta ttgtaggcca accagcaaag 181 cgtcaagctg taactaaccc aacaaacaca ttctttgcca ttaagcgttt aatcggccgt 241 cgttttaaag atgacgaagt acaacgtgac gtgaacatca tgccattcaa aattatcgga 301 gctgataatg gtgatgcatg ggttgaatca cgtggtaata aaatggcacc accacaagtt 361 tcggctgaaa tcttgaaaaa gatgaaaaag actgctgaag actttttagg tgaagaagtg 421 actgaagcgg ttattaccgt tcctgcttac tttaacgatt cacaacgtca agcaacgaaa 481 gatgctggtc gtatcgcagg tcttgatgtt aagcgtatta tcaacgaacc tactgctgct 541 gcacttgcat acggtatcga taagaagcaa ggcgacaaca ttgttgctgt atacgattta 601 ggtggtggta cattcgatat ttctatcatc gaaatcgaca gtaacgatgg cgaccaaaca 661 tttgaagtac tagcaaccaa tggtgatact cacttaggtg gtgaagactt tgataaccgt 721 atgattaact atttagctga tgagttcaaa aaagaccagg gcttagatct tcgtagagat 781 cctttagcaa tgcaacgttt gaaagaagcg gctgaaaaag caaaaatcga gctttcaagc 841 actaaccaca ctgaagttaa cttgccttac atcactgctg atgcatcagg tcctaagcat 901 ttagtggtta aaattactcg tgctaagtta gagtcattag ttgaagattt aattcaacgt 961 acgctagagc cgcttaaagt tgcactagct gatgctgatt tatcaatatc tgatatcaat 1021 gaagtgattc ttgttggtgg ccagactcgt atgcctaaag tacaagaagc agtaactaac 1081 ttcttcggca aagagcctcg taaagatgtt aaccctgatg aagcggttgc agttggcgcg 1141 gcgattcagg ctggcgtact ttctggtgaa gtgaaagacg tacttctact tgacgttaca 1201 ccactatctc ttggtattga aaccatgggc agtgtgatga ccaagcttat cgagaagaac 1261 accactatcc cgactaaagc tcagcaagta ttctcaacgg ctgacgacaa ccaaagtgca 1321 gtgacaattc acgtacttca aggtgagcgt aagcaagcta gtgccaacaa gtcattaggt 1381 caatttaacc tagaaggtat tgagccagca ccacgtggcc aaccacaggt tgaagtgatg 1441 ttcgacattg atgctgatgg tatcttacat gtgtctgcaa cagacaagaa aacaggtaag 1501 aaacaaaata ttactatcaa agcttcttca ggtttgtctg atgaagaagt tgaacaaatg 1561 gtacgtgatg cagaagctca tgctgatgaa gatgcgaaat ttgaagagtt agttcaagcg 1621 cgtaatcaag cagatggttt agctcattca actaaaaaac aagttgaaga agctggcgat 1681 gcactagcga gcgacgaaaa agaaaagatt gaagcggcaa ttgcaacttt agaaactgcc 1741 ataaaaggca aagataaaga agctattgat acagctactc aagcgttaat cgaagcgtct 1801 gctaagttaa tggaaattgc tcaagctaaa gctcaaggtg aagcagaagg tcaagcacac 1861 gatgctggcc aagaaaagcc tgctgatgat gttgttgatg ctgagttcga agaagttaaa 1921 gacgacaaaa agtaataa //