##gff-version 3
##sequence-region BBa_K538102 1 1938
BBa_K538102	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K538102	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K538102	.	CDS	19	1938	.	+	0	ID=BBa_K538002;Name=BBa_K538002
BBa_K538102	.	stop_codon	1933	1938	.	+	0	ID=annotation2124234;Name=Double Stop Codon;Parent=BBa_K538002
BBa_K538102	.	start_codon	19	21	.	+	0	ID=annotation2124233;Name=Start Codon;Parent=BBa_K538002
BBa_K538102	.	point_mutation	1299	1299	.	+	0	ID=annotation2127791;Name=A > G (Recode for PvuII site);Parent=BBa_K538002
BBa_K538102	.	point_mutation	744	744	.	+	0	ID=annotation2127789;Name=A > G (Recode for EcoRI site);Parent=BBa_K538002
BBa_K538102	.	point_mutation	363	363	.	+	0	ID=annotation2127787;Name=A > G (Recode for PvuII site);Parent=BBa_K538002
BBa_K538102	.	point_mutation	1689	1689	.	+	0	ID=annotation2127792;Name=T > G (Recode for NheI site);Parent=BBa_K538002
BBa_K538102	.	point_mutation	963	963	.	+	0	ID=annotation2127790;Name=T > G (Recode for XbaI site);Parent=BBa_K538002
>BBa_K538102
aaagaggagaaatactagatgggcaaaattattggtatcgatttaggcacaacaaactcgtgtgtagcagtccttgatg
gcggcaaagcacgcgtaattgaaaacgcagagggtgatcgtacaaccccatcaattatcgcttataccgatgatgaaat
tattgtaggccaaccagcaaagcgtcaagctgtaactaacccaacaaacacattctttgccattaagcgtttaatcggc
cgtcgttttaaagatgacgaagtacaacgtgacgtgaacatcatgccattcaaaattatcggagctgataatggtgatg
catgggttgaatcacgtggtaataaaatggcaccaccacaagtttcggctgaaatcttgaaaaagatgaaaaagactgc
tgaagactttttaggtgaagaagtgactgaagcggttattaccgttcctgcttactttaacgattcacaacgtcaagca
acgaaagatgctggtcgtatcgcaggtcttgatgttaagcgtattatcaacgaacctactgctgctgcacttgcatacg
gtatcgataagaagcaaggcgacaacattgttgctgtatacgatttaggtggtggtacattcgatatttctatcatcga
aatcgacagtaacgatggcgaccaaacatttgaagtactagcaaccaatggtgatactcacttaggtggtgaagacttt
gataaccgtatgattaactatttagctgatgagttcaaaaaagaccagggcttagatcttcgtagagatcctttagcaa
tgcaacgtttgaaagaagcggctgaaaaagcaaaaatcgagctttcaagcactaaccacactgaagttaacttgcctta
catcactgctgatgcatcaggtcctaagcatttagtggttaaaattactcgtgctaagttagagtcattagttgaagat
ttaattcaacgtacgctagagccgcttaaagttgcactagctgatgctgatttatcaatatctgatatcaatgaagtga
ttcttgttggtggccagactcgtatgcctaaagtacaagaagcagtaactaacttcttcggcaaagagcctcgtaaaga
tgttaaccctgatgaagcggttgcagttggcgcggcgattcaggctggcgtactttctggtgaagtgaaagacgtactt
ctacttgacgttacaccactatctcttggtattgaaaccatgggcagtgtgatgaccaagcttatcgagaagaacacca
ctatcccgactaaagctcagcaagtattctcaacggctgacgacaaccaaagtgcagtgacaattcacgtacttcaagg
tgagcgtaagcaagctagtgccaacaagtcattaggtcaatttaacctagaaggtattgagccagcaccacgtggccaa
ccacaggttgaagtgatgttcgacattgatgctgatggtatcttacatgtgtctgcaacagacaagaaaacaggtaaga
aacaaaatattactatcaaagcttcttcaggtttgtctgatgaagaagttgaacaaatggtacgtgatgcagaagctca
tgctgatgaagatgcgaaatttgaagagttagttcaagcgcgtaatcaagcagatggtttagctcattcaactaaaaaa
caagttgaagaagctggcgatgcactagcgagcgacgaaaaagaaaagattgaagcggcaattgcaactttagaaactg
ccataaaaggcaaagataaagaagctattgatacagctactcaagcgttaatcgaagcgtctgctaagttaatggaaat
tgctcaagctaaagctcaaggtgaagcagaaggtcaagcacacgatgctggccaagaaaagcctgctgatgatgttgtt
gatgctgagttcgaagaagttaaagacgacaaaaagtaataa