BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961227 1 start range1961227 1 173 173 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961225 1 -10 range1961225 1 161 166 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961224 1 -35 range1961224 1 137 142 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 BBa_K538201 1 LacI:Cpn60 pLacI + RBS + Cpn60 2011-09-07T11:00:00Z 2015-05-08T01:12:37Z - - false false _704_ 0 9985 9 Not in stock false - false Bas Stringer component2219865 1 BBa_R0010 component2219873 1 BBa_B0034 component2219877 1 BBa_K538001 annotation2219873 1 BBa_B0034 range2219873 1 209 220 annotation2219865 1 BBa_R0010 range2219865 1 1 200 annotation2219877 1 BBa_K538001 range2219877 1 227 1876 BBa_K538001 1 Cpn60 Cpn60 (O. antarctica) 2011-07-28T11:00:00Z 2015-05-08T01:12:36Z De novo synthesis - false false _704_ 0 9985 9 Not in stock false - false Bas Stringer (Sequence by Paul van Dieken) annotation2128866 1 A > G (Recode for SapI site) range2128866 1 908 908 annotation2124232 1 Double Stop Codon range2124232 1 1645 1650 annotation2124231 1 Start Codon range2124231 1 1 3 BBa_K538201_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatggctgctaaagacgtattatttggtgatagcgcacgcgcaaaaatgttggtaggtgtaaacattttagccgacgcagtaagagttaccttaggacctaaaggtcgtaacgttgttatagaaaaatcatttggtgcaccgatcatcaccaaagatggtgtttctgttgcgcgtgaaatcgaattgaaagacaaattcgaaaacatgggcgcacagatggttaaggaagttgcttctcaagccaacgaccaagccggtgacggcacaacgacagcgactgtactagcacaggcgattatcagcgaaggcttgaaatctgttgcggctggcatgaatccaatggatcttaaacgtggtattgataaagctacggctgctgttgttgccgccattaaagaacaagctcagccttgcttggatacaaaagcaatcgctcaggtagggacaatctctgccaatgccgatgaaacggttggtcgtttaattgctgaagcgatggaaaaagtcggtaaagaaggtgtgattaccgttgaagaaggcaaaggccttgaagacgagcttgatgttgtagaaggcatgcagttcgatcgcggttacttgtctccgtacttcatcaacaaccaagaaaaaatgaccgtagaaatggaaaatccattaattctattggttgataagaaaattgataaccttcaagagctgttgccaattcttgaaaacgtcgctaaatcaggtcgtccattattgatcgttgctgaagatgttgaaggccaagcactagcaacattggtagtaaacaacttgcgcggcacattcaaggttgcagcggttaaagcccctggttttggcgatcgtcgtaaagcgatgttgcaagatcttgccatcttgacgggtggtcaggttatttctgaggagctagggatgtctttagaaactgcggatccttcttctttgggtacggcaagcaaggttgttatcgataaagaaaacaccgtgattgttgatggcgcaggtactgaagcaagcgttaatactcgtgttgaccagatccgtgctgaaatcgaaagctcgacttctgattacgacatcgaaaagttacaagaacgcgttgctaagcttgcgggcggcgttgccgtgattaaggttggtgcgggttctgaaatggaaatgaaagagaagaaagaccgtgttgacgatgcacttcatgcaactcgcgcagcggttgaagaaggtgttgttgcgggtggtggtgttgctttgattcgcgcactctcttcagtaaccgttgttggtgataacgaagatcaaaacgtcggtattgcattggcacttcgtgcgatggaagctcctatccgtcaaatcgcgggtaacgcaggtgctgaagggtcagtggttgttgataaagtgaaatctggcacaggtagctttggttttaacgccagcacaggtgagtatggcgatatgattgcgatgggtattttagaccctgcaaaagtcacgcgttcatctctacaagccgcggcgtctatcgcaggtttgatgatcacaaccgaagccatggttgcggatgcgcctgttgaagaaggcgctggtggtatgcctgatatgggcggcatgggtggaatgggcggtatgcctggcatgatgtaataa BBa_K538001_sequence 1 atggctgctaaagacgtattatttggtgatagcgcacgcgcaaaaatgttggtaggtgtaaacattttagccgacgcagtaagagttaccttaggacctaaaggtcgtaacgttgttatagaaaaatcatttggtgcaccgatcatcaccaaagatggtgtttctgttgcgcgtgaaatcgaattgaaagacaaattcgaaaacatgggcgcacagatggttaaggaagttgcttctcaagccaacgaccaagccggtgacggcacaacgacagcgactgtactagcacaggcgattatcagcgaaggcttgaaatctgttgcggctggcatgaatccaatggatcttaaacgtggtattgataaagctacggctgctgttgttgccgccattaaagaacaagctcagccttgcttggatacaaaagcaatcgctcaggtagggacaatctctgccaatgccgatgaaacggttggtcgtttaattgctgaagcgatggaaaaagtcggtaaagaaggtgtgattaccgttgaagaaggcaaaggccttgaagacgagcttgatgttgtagaaggcatgcagttcgatcgcggttacttgtctccgtacttcatcaacaaccaagaaaaaatgaccgtagaaatggaaaatccattaattctattggttgataagaaaattgataaccttcaagagctgttgccaattcttgaaaacgtcgctaaatcaggtcgtccattattgatcgttgctgaagatgttgaaggccaagcactagcaacattggtagtaaacaacttgcgcggcacattcaaggttgcagcggttaaagcccctggttttggcgatcgtcgtaaagcgatgttgcaagatcttgccatcttgacgggtggtcaggttatttctgaggagctagggatgtctttagaaactgcggatccttcttctttgggtacggcaagcaaggttgttatcgataaagaaaacaccgtgattgttgatggcgcaggtactgaagcaagcgttaatactcgtgttgaccagatccgtgctgaaatcgaaagctcgacttctgattacgacatcgaaaagttacaagaacgcgttgctaagcttgcgggcggcgttgccgtgattaaggttggtgcgggttctgaaatggaaatgaaagagaagaaagaccgtgttgacgatgcacttcatgcaactcgcgcagcggttgaagaaggtgttgttgcgggtggtggtgttgctttgattcgcgcactctcttcagtaaccgttgttggtgataacgaagatcaaaacgtcggtattgcattggcacttcgtgcgatggaagctcctatccgtcaaatcgcgggtaacgcaggtgctgaagggtcagtggttgttgataaagtgaaatctggcacaggtagctttggttttaacgccagcacaggtgagtatggcgatatgattgcgatgggtattttagaccctgcaaaagtcacgcgttcatctctacaagccgcggcgtctatcgcaggtttgatgatcacaaccgaagccatggttgcggatgcgcctgttgaagaaggcgctggtggtatgcctgatatgggcggcatgggtggaatgggcggtatgcctggcatgatgtaataa BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_B0034_sequence 1 aaagaggagaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z