##gff-version 3
##sequence-region BBa_K538202 1 2146
BBa_K538202	.	CDS	227	2146	.	+	0	ID=BBa_K538002;Name=BBa_K538002
BBa_K538202	.	point_mutation	571	571	.	+	0	ID=annotation2127787;Name=A > G (Recode for PvuII site);Parent=BBa_K538002
BBa_K538202	.	start_codon	227	229	.	+	0	ID=annotation2124233;Name=Start Codon;Parent=BBa_K538002
BBa_K538202	.	point_mutation	952	952	.	+	0	ID=annotation2127789;Name=A > G (Recode for EcoRI site);Parent=BBa_K538002
BBa_K538202	.	point_mutation	1171	1171	.	+	0	ID=annotation2127790;Name=T > G (Recode for XbaI site);Parent=BBa_K538002
BBa_K538202	.	point_mutation	1507	1507	.	+	0	ID=annotation2127791;Name=A > G (Recode for PvuII site);Parent=BBa_K538002
BBa_K538202	.	point_mutation	1897	1897	.	+	0	ID=annotation2127792;Name=T > G (Recode for NheI site);Parent=BBa_K538002
BBa_K538202	.	stop_codon	2141	2146	.	+	0	ID=annotation2124234;Name=Double Stop Codon;Parent=BBa_K538002
BBa_K538202	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K538202	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K538202	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K538202	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K538202	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K538202	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K538202	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K538202	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K538202	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K538202	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
>BBa_K538202
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgggcaaaat
tattggtatcgatttaggcacaacaaactcgtgtgtagcagtccttgatggcggcaaagcacgcgtaattgaaaacgca
gagggtgatcgtacaaccccatcaattatcgcttataccgatgatgaaattattgtaggccaaccagcaaagcgtcaag
ctgtaactaacccaacaaacacattctttgccattaagcgtttaatcggccgtcgttttaaagatgacgaagtacaacg
tgacgtgaacatcatgccattcaaaattatcggagctgataatggtgatgcatgggttgaatcacgtggtaataaaatg
gcaccaccacaagtttcggctgaaatcttgaaaaagatgaaaaagactgctgaagactttttaggtgaagaagtgactg
aagcggttattaccgttcctgcttactttaacgattcacaacgtcaagcaacgaaagatgctggtcgtatcgcaggtct
tgatgttaagcgtattatcaacgaacctactgctgctgcacttgcatacggtatcgataagaagcaaggcgacaacatt
gttgctgtatacgatttaggtggtggtacattcgatatttctatcatcgaaatcgacagtaacgatggcgaccaaacat
ttgaagtactagcaaccaatggtgatactcacttaggtggtgaagactttgataaccgtatgattaactatttagctga
tgagttcaaaaaagaccagggcttagatcttcgtagagatcctttagcaatgcaacgtttgaaagaagcggctgaaaaa
gcaaaaatcgagctttcaagcactaaccacactgaagttaacttgccttacatcactgctgatgcatcaggtcctaagc
atttagtggttaaaattactcgtgctaagttagagtcattagttgaagatttaattcaacgtacgctagagccgcttaa
agttgcactagctgatgctgatttatcaatatctgatatcaatgaagtgattcttgttggtggccagactcgtatgcct
aaagtacaagaagcagtaactaacttcttcggcaaagagcctcgtaaagatgttaaccctgatgaagcggttgcagttg
gcgcggcgattcaggctggcgtactttctggtgaagtgaaagacgtacttctacttgacgttacaccactatctcttgg
tattgaaaccatgggcagtgtgatgaccaagcttatcgagaagaacaccactatcccgactaaagctcagcaagtattc
tcaacggctgacgacaaccaaagtgcagtgacaattcacgtacttcaaggtgagcgtaagcaagctagtgccaacaagt
cattaggtcaatttaacctagaaggtattgagccagcaccacgtggccaaccacaggttgaagtgatgttcgacattga
tgctgatggtatcttacatgtgtctgcaacagacaagaaaacaggtaagaaacaaaatattactatcaaagcttcttca
ggtttgtctgatgaagaagttgaacaaatggtacgtgatgcagaagctcatgctgatgaagatgcgaaatttgaagagt
tagttcaagcgcgtaatcaagcagatggtttagctcattcaactaaaaaacaagttgaagaagctggcgatgcactagc
gagcgacgaaaaagaaaagattgaagcggcaattgcaactttagaaactgccataaaaggcaaagataaagaagctatt
gatacagctactcaagcgttaatcgaagcgtctgctaagttaatggaaattgctcaagctaaagctcaaggtgaagcag
aaggtcaagcacacgatgctggccaagaaaagcctgctgatgatgttgttgatgctgagttcgaagaagttaaagacga
caaaaagtaataa