LOCUS       BBa_K538210             2199 bp    DNA     linear       22-Dec-2024
DEFINITION  Cpn10/60 operon (pLacI)
ACCESSION   BBa_K538210
VERSION     BBa_K538210.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..229
                     /label=Start Codon
     misc_feature    518..523
                     /label=Double Stop Codon
     CDS             227..523
                     /label=BBa_K538000
     misc_feature    536..539
                     /label=conserved
     RBS             532..543
                     /label=BBa_B0034
     misc_feature    550..552
                     /label=Start Codon
     misc_feature    2194..2199
                     /label=Double Stop Codon
     misc_feature    1457..1457
                     /label=A > G (Recode for SapI site)
     CDS             550..2199
                     /label=BBa_K538001
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aaatccgtcc
      241 attacatgat cgtattgttg ttcgccgtaa agaagaagag accgcaactg cgggtggtat
      301 tattttaccg ggcgctgcgg cagaaaaacc aaatcaaggt gttgttatct ctgtgggtac
      361 tggccgtatt cttgataatg gttcagtgca agcgctggcg gttaacgaag gcgatgttgt
      421 cgtttttggt aaatactcag gtcaaaatac tatcgatatc gatggtgaag aattattgat
      481 tttgaatgaa agtgatatct acggcgtttt agaagcttaa taatactaga gaaagaggag
      541 aaatactaga tggctgctaa agacgtatta tttggtgata gcgcacgcgc aaaaatgttg
      601 gtaggtgtaa acattttagc cgacgcagta agagttacct taggacctaa aggtcgtaac
      661 gttgttatag aaaaatcatt tggtgcaccg atcatcacca aagatggtgt ttctgttgcg
      721 cgtgaaatcg aattgaaaga caaattcgaa aacatgggcg cacagatggt taaggaagtt
      781 gcttctcaag ccaacgacca agccggtgac ggcacaacga cagcgactgt actagcacag
      841 gcgattatca gcgaaggctt gaaatctgtt gcggctggca tgaatccaat ggatcttaaa
      901 cgtggtattg ataaagctac ggctgctgtt gttgccgcca ttaaagaaca agctcagcct
      961 tgcttggata caaaagcaat cgctcaggta gggacaatct ctgccaatgc cgatgaaacg
     1021 gttggtcgtt taattgctga agcgatggaa aaagtcggta aagaaggtgt gattaccgtt
     1081 gaagaaggca aaggccttga agacgagctt gatgttgtag aaggcatgca gttcgatcgc
     1141 ggttacttgt ctccgtactt catcaacaac caagaaaaaa tgaccgtaga aatggaaaat
     1201 ccattaattc tattggttga taagaaaatt gataaccttc aagagctgtt gccaattctt
     1261 gaaaacgtcg ctaaatcagg tcgtccatta ttgatcgttg ctgaagatgt tgaaggccaa
     1321 gcactagcaa cattggtagt aaacaacttg cgcggcacat tcaaggttgc agcggttaaa
     1381 gcccctggtt ttggcgatcg tcgtaaagcg atgttgcaag atcttgccat cttgacgggt
     1441 ggtcaggtta tttctgagga gctagggatg tctttagaaa ctgcggatcc ttcttctttg
     1501 ggtacggcaa gcaaggttgt tatcgataaa gaaaacaccg tgattgttga tggcgcaggt
     1561 actgaagcaa gcgttaatac tcgtgttgac cagatccgtg ctgaaatcga aagctcgact
     1621 tctgattacg acatcgaaaa gttacaagaa cgcgttgcta agcttgcggg cggcgttgcc
     1681 gtgattaagg ttggtgcggg ttctgaaatg gaaatgaaag agaagaaaga ccgtgttgac
     1741 gatgcacttc atgcaactcg cgcagcggtt gaagaaggtg ttgttgcggg tggtggtgtt
     1801 gctttgattc gcgcactctc ttcagtaacc gttgttggtg ataacgaaga tcaaaacgtc
     1861 ggtattgcat tggcacttcg tgcgatggaa gctcctatcc gtcaaatcgc gggtaacgca
     1921 ggtgctgaag ggtcagtggt tgttgataaa gtgaaatctg gcacaggtag ctttggtttt
     1981 aacgccagca caggtgagta tggcgatatg attgcgatgg gtattttaga ccctgcaaaa
     2041 gtcacgcgtt catctctaca agccgcggcg tctatcgcag gtttgatgat cacaaccgaa
     2101 gccatggttg cggatgcgcc tgttgaagaa ggcgctggtg gtatgcctga tatgggcggc
     2161 atgggtggaa tgggcggtat gcctggcatg atgtaataa
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