BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K538210 1 Cpn10/60 Cpn10/60 operon (pLacI) 2011-09-07T11:00:00Z 2015-05-08T01:12:37Z - - false false _704_ 0 9985 9 Not in stock false - false Bas Stringer component2222764 1 BBa_B0034 component2222769 1 BBa_B0034 component2222756 1 BBa_R0010 component2222767 1 BBa_K538000 component2222773 1 BBa_K538001 annotation2222756 1 BBa_R0010 range2222756 1 1 200 annotation2222767 1 BBa_K538000 range2222767 1 227 523 annotation2222773 1 BBa_K538001 range2222773 1 550 2199 annotation2222764 1 BBa_B0034 range2222764 1 209 220 annotation2222769 1 BBa_B0034 range2222769 1 532 543 BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961224 1 -35 range1961224 1 137 142 annotation1961227 1 start range1961227 1 173 173 annotation1961225 1 -10 range1961225 1 161 166 BBa_K538001 1 Cpn60 Cpn60 (O. antarctica) 2011-07-28T11:00:00Z 2015-05-08T01:12:36Z De novo synthesis - false false _704_ 0 9985 9 Not in stock false - false Bas Stringer (Sequence by Paul van Dieken) annotation2128866 1 A > G (Recode for SapI site) range2128866 1 908 908 annotation2124231 1 Start Codon range2124231 1 1 3 annotation2124232 1 Double Stop Codon range2124232 1 1645 1650 BBa_K538000 1 Cpn10 Cpn10 (O. antarctica) 2011-07-27T11:00:00Z 2015-05-08T01:12:36Z ''De novo'' synthesis Released HQ 2013 This part encodes the protein ''cochaperonin 10'', which is part of the Cpn10/Cpn60 chaperonin system of ''Oleispira antarctica''. [More information will follow soon.] false false _704_ 0 9985 9 In stock true The protein's sequence[1] was published by Ferrer ''et al.'' in 2004. Pre- and suffixes were added to this as clarified in OpenWetWare's BioBrick standards[2] and, as is recommended, the TAA stop codon was replaced with TAATAA. The sequence's conformity with Assembly standard 10[3] was confirmed using the EMBOSS recoder[4], by recoding restriction sites of EcoRI, XbaI, SpeI, PstI, NotI, PvuII, XhoI, AvrII, NheI and SapI. 1 - http://www.ncbi.nlm.nih.gov/nuccore/22266159?from=458&to=751&report=gbwithparts 2 - http://openwetware.org/wiki/Biobrick_standard 3 - http://partsregistry.org/Help:Assembly_standard_10 4 - http://bioweb2.pasteur.fr/docs/EMBOSS/recoder.html false Bas Stringer (Sequence by Paul van Dieken) annotation2124175 1 Double Stop Codon range2124175 1 292 297 annotation2124174 1 Start Codon range2124174 1 1 3 BBa_K538001_sequence 1 atggctgctaaagacgtattatttggtgatagcgcacgcgcaaaaatgttggtaggtgtaaacattttagccgacgcagtaagagttaccttaggacctaaaggtcgtaacgttgttatagaaaaatcatttggtgcaccgatcatcaccaaagatggtgtttctgttgcgcgtgaaatcgaattgaaagacaaattcgaaaacatgggcgcacagatggttaaggaagttgcttctcaagccaacgaccaagccggtgacggcacaacgacagcgactgtactagcacaggcgattatcagcgaaggcttgaaatctgttgcggctggcatgaatccaatggatcttaaacgtggtattgataaagctacggctgctgttgttgccgccattaaagaacaagctcagccttgcttggatacaaaagcaatcgctcaggtagggacaatctctgccaatgccgatgaaacggttggtcgtttaattgctgaagcgatggaaaaagtcggtaaagaaggtgtgattaccgttgaagaaggcaaaggccttgaagacgagcttgatgttgtagaaggcatgcagttcgatcgcggttacttgtctccgtacttcatcaacaaccaagaaaaaatgaccgtagaaatggaaaatccattaattctattggttgataagaaaattgataaccttcaagagctgttgccaattcttgaaaacgtcgctaaatcaggtcgtccattattgatcgttgctgaagatgttgaaggccaagcactagcaacattggtagtaaacaacttgcgcggcacattcaaggttgcagcggttaaagcccctggttttggcgatcgtcgtaaagcgatgttgcaagatcttgccatcttgacgggtggtcaggttatttctgaggagctagggatgtctttagaaactgcggatccttcttctttgggtacggcaagcaaggttgttatcgataaagaaaacaccgtgattgttgatggcgcaggtactgaagcaagcgttaatactcgtgttgaccagatccgtgctgaaatcgaaagctcgacttctgattacgacatcgaaaagttacaagaacgcgttgctaagcttgcgggcggcgttgccgtgattaaggttggtgcgggttctgaaatggaaatgaaagagaagaaagaccgtgttgacgatgcacttcatgcaactcgcgcagcggttgaagaaggtgttgttgcgggtggtggtgttgctttgattcgcgcactctcttcagtaaccgttgttggtgataacgaagatcaaaacgtcggtattgcattggcacttcgtgcgatggaagctcctatccgtcaaatcgcgggtaacgcaggtgctgaagggtcagtggttgttgataaagtgaaatctggcacaggtagctttggttttaacgccagcacaggtgagtatggcgatatgattgcgatgggtattttagaccctgcaaaagtcacgcgttcatctctacaagccgcggcgtctatcgcaggtttgatgatcacaaccgaagccatggttgcggatgcgcctgttgaagaaggcgctggtggtatgcctgatatgggcggcatgggtggaatgggcggtatgcctggcatgatgtaataa BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_B0034_sequence 1 aaagaggagaaa BBa_K538210_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaaaatccgtccattacatgatcgtattgttgttcgccgtaaagaagaagagaccgcaactgcgggtggtattattttaccgggcgctgcggcagaaaaaccaaatcaaggtgttgttatctctgtgggtactggccgtattcttgataatggttcagtgcaagcgctggcggttaacgaaggcgatgttgtcgtttttggtaaatactcaggtcaaaatactatcgatatcgatggtgaagaattattgattttgaatgaaagtgatatctacggcgttttagaagcttaataatactagagaaagaggagaaatactagatggctgctaaagacgtattatttggtgatagcgcacgcgcaaaaatgttggtaggtgtaaacattttagccgacgcagtaagagttaccttaggacctaaaggtcgtaacgttgttatagaaaaatcatttggtgcaccgatcatcaccaaagatggtgtttctgttgcgcgtgaaatcgaattgaaagacaaattcgaaaacatgggcgcacagatggttaaggaagttgcttctcaagccaacgaccaagccggtgacggcacaacgacagcgactgtactagcacaggcgattatcagcgaaggcttgaaatctgttgcggctggcatgaatccaatggatcttaaacgtggtattgataaagctacggctgctgttgttgccgccattaaagaacaagctcagccttgcttggatacaaaagcaatcgctcaggtagggacaatctctgccaatgccgatgaaacggttggtcgtttaattgctgaagcgatggaaaaagtcggtaaagaaggtgtgattaccgttgaagaaggcaaaggccttgaagacgagcttgatgttgtagaaggcatgcagttcgatcgcggttacttgtctccgtacttcatcaacaaccaagaaaaaatgaccgtagaaatggaaaatccattaattctattggttgataagaaaattgataaccttcaagagctgttgccaattcttgaaaacgtcgctaaatcaggtcgtccattattgatcgttgctgaagatgttgaaggccaagcactagcaacattggtagtaaacaacttgcgcggcacattcaaggttgcagcggttaaagcccctggttttggcgatcgtcgtaaagcgatgttgcaagatcttgccatcttgacgggtggtcaggttatttctgaggagctagggatgtctttagaaactgcggatccttcttctttgggtacggcaagcaaggttgttatcgataaagaaaacaccgtgattgttgatggcgcaggtactgaagcaagcgttaatactcgtgttgaccagatccgtgctgaaatcgaaagctcgacttctgattacgacatcgaaaagttacaagaacgcgttgctaagcttgcgggcggcgttgccgtgattaaggttggtgcgggttctgaaatggaaatgaaagagaagaaagaccgtgttgacgatgcacttcatgcaactcgcgcagcggttgaagaaggtgttgttgcgggtggtggtgttgctttgattcgcgcactctcttcagtaaccgttgttggtgataacgaagatcaaaacgtcggtattgcattggcacttcgtgcgatggaagctcctatccgtcaaatcgcgggtaacgcaggtgctgaagggtcagtggttgttgataaagtgaaatctggcacaggtagctttggttttaacgccagcacaggtgagtatggcgatatgattgcgatgggtattttagaccctgcaaaagtcacgcgttcatctctacaagccgcggcgtctatcgcaggtttgatgatcacaaccgaagccatggttgcggatgcgcctgttgaagaaggcgctggtggtatgcctgatatgggcggcatgggtggaatgggcggtatgcctggcatgatgtaataa BBa_K538000_sequence 1 atgaaaatccgtccattacatgatcgtattgttgttcgccgtaaagaagaagagaccgcaactgcgggtggtattattttaccgggcgctgcggcagaaaaaccaaatcaaggtgttgttatctctgtgggtactggccgtattcttgataatggttcagtgcaagcgctggcggttaacgaaggcgatgttgtcgtttttggtaaatactcaggtcaaaatactatcgatatcgatggtgaagaattattgattttgaatgaaagtgatatctacggcgttttagaagcttaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z