BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K538000
1
Cpn10
Cpn10 (O. antarctica)
2011-07-27T11:00:00Z
2015-05-08T01:12:36Z
''De novo'' synthesis
Released HQ 2013
This part encodes the protein ''cochaperonin 10'', which is part of the Cpn10/Cpn60 chaperonin system of ''Oleispira antarctica''.
[More information will follow soon.]
false
false
_704_
0
9985
9
In stock
true
The protein's sequence[1] was published by Ferrer ''et al.'' in 2004. Pre- and suffixes were added to this as clarified in OpenWetWare's BioBrick standards[2] and, as is recommended, the TAA stop codon was replaced with TAATAA. The sequence's conformity with Assembly standard 10[3] was confirmed using the EMBOSS recoder[4], by recoding restriction sites of EcoRI, XbaI, SpeI, PstI, NotI, PvuII, XhoI, AvrII, NheI and SapI.
1 - http://www.ncbi.nlm.nih.gov/nuccore/22266159?from=458&to=751&report=gbwithparts
2 - http://openwetware.org/wiki/Biobrick_standard
3 - http://partsregistry.org/Help:Assembly_standard_10
4 - http://bioweb2.pasteur.fr/docs/EMBOSS/recoder.html
false
Bas Stringer (Sequence by Paul van Dieken)
annotation2124174
1
Start Codon
range2124174
1
1
3
annotation2124175
1
Double Stop Codon
range2124175
1
292
297
BBa_K206000
1
pBAD
pBAD strong
2009-10-13T11:00:00Z
2015-05-08T01:11:23Z
The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1].
Released HQ 2013
pBAD is an <i>E.coli</i> promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter.
false
false
_307_
0
4172
9
In stock
true
There were no special design considerations.
false
Amelia Hardjasa
annotation2049252
1
promoter
range2049252
1
1
131
annotation2049253
1
AraI1
range2049253
1
40
57
annotation2049254
1
AraI2
range2049254
1
61
78
BBa_K538300
1
pBAD:Cpn10
pBAD + RBS + Cpn10
2011-09-07T11:00:00Z
2015-05-08T01:12:37Z
-
-
false
false
_704_
0
9985
9
Not in stock
false
-
false
Bas Stringer
component2126761
1
BBa_B0034
component2126759
1
BBa_K206000
component2126764
1
BBa_K538000
annotation2126761
1
BBa_B0034
range2126761
1
139
150
annotation2126764
1
BBa_K538000
range2126764
1
157
453
annotation2126759
1
BBa_K206000
range2126759
1
1
130
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K538300_sequence
1
acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgaaaatccgtccattacatgatcgtattgttgttcgccgtaaagaagaagagaccgcaactgcgggtggtattattttaccgggcgctgcggcagaaaaaccaaatcaaggtgttgttatctctgtgggtactggccgtattcttgataatggttcagtgcaagcgctggcggttaacgaaggcgatgttgtcgtttttggtaaatactcaggtcaaaatactatcgatatcgatggtgaagaattattgattttgaatgaaagtgatatctacggcgttttagaagcttaataa
BBa_K206000_sequence
1
acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
BBa_K538000_sequence
1
atgaaaatccgtccattacatgatcgtattgttgttcgccgtaaagaagaagagaccgcaactgcgggtggtattattttaccgggcgctgcggcagaaaaaccaaatcaaggtgttgttatctctgtgggtactggccgtattcttgataatggttcagtgcaagcgctggcggttaacgaaggcgatgttgtcgtttttggtaaatactcaggtcaaaatactatcgatatcgatggtgaagaattattgattttgaatgaaagtgatatctacggcgttttagaagcttaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z