BBa_K538002 1 SheDnaK SheDnaK (Shewanella sp. Ac10) 2011-07-28T11:00:00Z 2015-05-08T01:12:36Z - - false false _704_ 0 9985 9 Not in stock false - false Bas Stringer (Sequence by Paul van Dieken) annotation2124234 1 Double Stop Codon range2124234 1 1915 1920 annotation2124233 1 Start Codon range2124233 1 1 3 annotation2127789 1 A > G (Recode for EcoRI site) range2127789 1 726 726 annotation2127791 1 A > G (Recode for PvuII site) range2127791 1 1281 1281 annotation2127792 1 T > G (Recode for NheI site) range2127792 1 1671 1671 annotation2127787 1 A > G (Recode for PvuII site) range2127787 1 345 345 annotation2127790 1 T > G (Recode for XbaI site) range2127790 1 945 945 BBa_K206000 1 pBAD pBAD strong 2009-10-13T11:00:00Z 2015-05-08T01:11:23Z The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1]. Released HQ 2013 pBAD is an <i>E.coli</i> promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter. false false _307_ 0 4172 9 In stock true There were no special design considerations. false Amelia Hardjasa annotation2049254 1 AraI2 range2049254 1 61 78 annotation2049253 1 AraI1 range2049253 1 40 57 annotation2049252 1 promoter range2049252 1 1 131 BBa_K538302 1 pBAD:SDnaK pBAD + RBS + SheDnaK 2011-09-07T11:00:00Z 2015-05-08T01:12:37Z - - false false _704_ 0 9985 9 Not in stock false - false Bas Stringer component2126779 1 BBa_B0034 component2126782 1 BBa_K538002 component2126777 1 BBa_K206000 annotation2126779 1 BBa_B0034 range2126779 1 139 150 annotation2126777 1 BBa_K206000 range2126777 1 1 130 annotation2126782 1 BBa_K538002 range2126782 1 157 2076 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0034_sequence 1 aaagaggagaaa BBa_K538302_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgggcaaaattattggtatcgatttaggcacaacaaactcgtgtgtagcagtccttgatggcggcaaagcacgcgtaattgaaaacgcagagggtgatcgtacaaccccatcaattatcgcttataccgatgatgaaattattgtaggccaaccagcaaagcgtcaagctgtaactaacccaacaaacacattctttgccattaagcgtttaatcggccgtcgttttaaagatgacgaagtacaacgtgacgtgaacatcatgccattcaaaattatcggagctgataatggtgatgcatgggttgaatcacgtggtaataaaatggcaccaccacaagtttcggctgaaatcttgaaaaagatgaaaaagactgctgaagactttttaggtgaagaagtgactgaagcggttattaccgttcctgcttactttaacgattcacaacgtcaagcaacgaaagatgctggtcgtatcgcaggtcttgatgttaagcgtattatcaacgaacctactgctgctgcacttgcatacggtatcgataagaagcaaggcgacaacattgttgctgtatacgatttaggtggtggtacattcgatatttctatcatcgaaatcgacagtaacgatggcgaccaaacatttgaagtactagcaaccaatggtgatactcacttaggtggtgaagactttgataaccgtatgattaactatttagctgatgagttcaaaaaagaccagggcttagatcttcgtagagatcctttagcaatgcaacgtttgaaagaagcggctgaaaaagcaaaaatcgagctttcaagcactaaccacactgaagttaacttgccttacatcactgctgatgcatcaggtcctaagcatttagtggttaaaattactcgtgctaagttagagtcattagttgaagatttaattcaacgtacgctagagccgcttaaagttgcactagctgatgctgatttatcaatatctgatatcaatgaagtgattcttgttggtggccagactcgtatgcctaaagtacaagaagcagtaactaacttcttcggcaaagagcctcgtaaagatgttaaccctgatgaagcggttgcagttggcgcggcgattcaggctggcgtactttctggtgaagtgaaagacgtacttctacttgacgttacaccactatctcttggtattgaaaccatgggcagtgtgatgaccaagcttatcgagaagaacaccactatcccgactaaagctcagcaagtattctcaacggctgacgacaaccaaagtgcagtgacaattcacgtacttcaaggtgagcgtaagcaagctagtgccaacaagtcattaggtcaatttaacctagaaggtattgagccagcaccacgtggccaaccacaggttgaagtgatgttcgacattgatgctgatggtatcttacatgtgtctgcaacagacaagaaaacaggtaagaaacaaaatattactatcaaagcttcttcaggtttgtctgatgaagaagttgaacaaatggtacgtgatgcagaagctcatgctgatgaagatgcgaaatttgaagagttagttcaagcgcgtaatcaagcagatggtttagctcattcaactaaaaaacaagttgaagaagctggcgatgcactagcgagcgacgaaaaagaaaagattgaagcggcaattgcaactttagaaactgccataaaaggcaaagataaagaagctattgatacagctactcaagcgttaatcgaagcgtctgctaagttaatggaaattgctcaagctaaagctcaaggtgaagcagaaggtcaagcacacgatgctggccaagaaaagcctgctgatgatgttgttgatgctgagttcgaagaagttaaagacgacaaaaagtaataa BBa_K538002_sequence 1 atgggcaaaattattggtatcgatttaggcacaacaaactcgtgtgtagcagtccttgatggcggcaaagcacgcgtaattgaaaacgcagagggtgatcgtacaaccccatcaattatcgcttataccgatgatgaaattattgtaggccaaccagcaaagcgtcaagctgtaactaacccaacaaacacattctttgccattaagcgtttaatcggccgtcgttttaaagatgacgaagtacaacgtgacgtgaacatcatgccattcaaaattatcggagctgataatggtgatgcatgggttgaatcacgtggtaataaaatggcaccaccacaagtttcggctgaaatcttgaaaaagatgaaaaagactgctgaagactttttaggtgaagaagtgactgaagcggttattaccgttcctgcttactttaacgattcacaacgtcaagcaacgaaagatgctggtcgtatcgcaggtcttgatgttaagcgtattatcaacgaacctactgctgctgcacttgcatacggtatcgataagaagcaaggcgacaacattgttgctgtatacgatttaggtggtggtacattcgatatttctatcatcgaaatcgacagtaacgatggcgaccaaacatttgaagtactagcaaccaatggtgatactcacttaggtggtgaagactttgataaccgtatgattaactatttagctgatgagttcaaaaaagaccagggcttagatcttcgtagagatcctttagcaatgcaacgtttgaaagaagcggctgaaaaagcaaaaatcgagctttcaagcactaaccacactgaagttaacttgccttacatcactgctgatgcatcaggtcctaagcatttagtggttaaaattactcgtgctaagttagagtcattagttgaagatttaattcaacgtacgctagagccgcttaaagttgcactagctgatgctgatttatcaatatctgatatcaatgaagtgattcttgttggtggccagactcgtatgcctaaagtacaagaagcagtaactaacttcttcggcaaagagcctcgtaaagatgttaaccctgatgaagcggttgcagttggcgcggcgattcaggctggcgtactttctggtgaagtgaaagacgtacttctacttgacgttacaccactatctcttggtattgaaaccatgggcagtgtgatgaccaagcttatcgagaagaacaccactatcccgactaaagctcagcaagtattctcaacggctgacgacaaccaaagtgcagtgacaattcacgtacttcaaggtgagcgtaagcaagctagtgccaacaagtcattaggtcaatttaacctagaaggtattgagccagcaccacgtggccaaccacaggttgaagtgatgttcgacattgatgctgatggtatcttacatgtgtctgcaacagacaagaaaacaggtaagaaacaaaatattactatcaaagcttcttcaggtttgtctgatgaagaagttgaacaaatggtacgtgatgcagaagctcatgctgatgaagatgcgaaatttgaagagttagttcaagcgcgtaatcaagcagatggtttagctcattcaactaaaaaacaagttgaagaagctggcgatgcactagcgagcgacgaaaaagaaaagattgaagcggcaattgcaactttagaaactgccataaaaggcaaagataaagaagctattgatacagctactcaagcgttaatcgaagcgtctgctaagttaatggaaattgctcaagctaaagctcaaggtgaagcagaaggtcaagcacacgatgctggccaagaaaagcctgctgatgatgttgttgatgctgagttcgaagaagttaaagacgacaaaaagtaataa BBa_K206000_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z