BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K538303 1 pBAD:CspA pBAD + RBS + CspA 2011-09-07T11:00:00Z 2015-05-08T01:12:37Z - - false false _704_ 0 9985 9 Not in stock false - false Bas Stringer component2134090 1 BBa_K206000 component2134092 1 BBa_B0034 component2134097 1 BBa_K538003 annotation2134090 1 BBa_K206000 range2134090 1 1 130 annotation2134092 1 BBa_B0034 range2134092 1 139 150 annotation2134097 1 BBa_K538003 range2134097 1 157 351 BBa_K206000 1 pBAD pBAD strong 2009-10-13T11:00:00Z 2015-05-08T01:11:23Z The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1]. Released HQ 2013 pBAD is an <i>E.coli</i> promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter. false false _307_ 0 4172 9 In stock true There were no special design considerations. false Amelia Hardjasa annotation2049252 1 promoter range2049252 1 1 131 annotation2049253 1 AraI1 range2049253 1 40 57 annotation2049254 1 AraI2 range2049254 1 61 78 BBa_K538003 1 CspA CspA (P. irgensii) 2011-09-07T11:00:00Z 2015-05-08T01:12:36Z - - false false _704_ 0 9985 9 Not in stock false - false Bas Stringer (Sequence by Paul van Dieken) annotation2129603 1 RNA binding motif (RPN1) range2129603 1 37 54 annotation2129604 1 RNA binding motif (RPN2) range2129604 1 79 93 annotation2127781 1 Start Codon range2127781 1 1 3 annotation2127782 1 Double Stop Codon range2127782 1 190 195 BBa_B0034_sequence 1 aaagaggagaaa BBa_K538303_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagatgagtaaaggtacagtaaagtttttcaatgaatctaagggatttggatttattacagaagaaggagttgataaagatcatttcgtacacatttctggattagtagatgaaatcagagaaggtgaccaagttgaatttgacttacaagaaggaaacaaaggattaaatgcagttaacgtaaaagttatctaataa BBa_K206000_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc BBa_K538003_sequence 1 atgagtaaaggtacagtaaagtttttcaatgaatctaagggatttggatttattacagaagaaggagttgataaagatcatttcgtacacatttctggattagtagatgaaatcagagaaggtgaccaagttgaatttgacttacaagaaggaaacaaaggattaaatgcagttaacgtaaaagttatctaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z