LOCUS       BBa_K538310             2129 bp    DNA     linear       26-Dec-2024
DEFINITION  Cpn10/60 operon (pBAD)
ACCESSION   BBa_K538310
VERSION     BBa_K538310.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206000
     misc_feature    143..146
                     /label=conserved
     RBS             139..150
                     /label=BBa_B0034
     misc_feature    157..159
                     /label=Start Codon
     misc_feature    448..453
                     /label=Double Stop Codon
     CDS             157..453
                     /label=BBa_K538000
     misc_feature    466..469
                     /label=conserved
     RBS             462..473
                     /label=BBa_B0034
     misc_feature    480..482
                     /label=Start Codon
     misc_feature    2124..2129
                     /label=Double Stop Codon
     misc_feature    1387..1387
                     /label=A > G (Recode for SapI site)
     CDS             480..2129
                     /label=BBa_K538001
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagaa agaggagaaa tactagatga aaatccgtcc attacatgat
      181 cgtattgttg ttcgccgtaa agaagaagag accgcaactg cgggtggtat tattttaccg
      241 ggcgctgcgg cagaaaaacc aaatcaaggt gttgttatct ctgtgggtac tggccgtatt
      301 cttgataatg gttcagtgca agcgctggcg gttaacgaag gcgatgttgt cgtttttggt
      361 aaatactcag gtcaaaatac tatcgatatc gatggtgaag aattattgat tttgaatgaa
      421 agtgatatct acggcgtttt agaagcttaa taatactaga gaaagaggag aaatactaga
      481 tggctgctaa agacgtatta tttggtgata gcgcacgcgc aaaaatgttg gtaggtgtaa
      541 acattttagc cgacgcagta agagttacct taggacctaa aggtcgtaac gttgttatag
      601 aaaaatcatt tggtgcaccg atcatcacca aagatggtgt ttctgttgcg cgtgaaatcg
      661 aattgaaaga caaattcgaa aacatgggcg cacagatggt taaggaagtt gcttctcaag
      721 ccaacgacca agccggtgac ggcacaacga cagcgactgt actagcacag gcgattatca
      781 gcgaaggctt gaaatctgtt gcggctggca tgaatccaat ggatcttaaa cgtggtattg
      841 ataaagctac ggctgctgtt gttgccgcca ttaaagaaca agctcagcct tgcttggata
      901 caaaagcaat cgctcaggta gggacaatct ctgccaatgc cgatgaaacg gttggtcgtt
      961 taattgctga agcgatggaa aaagtcggta aagaaggtgt gattaccgtt gaagaaggca
     1021 aaggccttga agacgagctt gatgttgtag aaggcatgca gttcgatcgc ggttacttgt
     1081 ctccgtactt catcaacaac caagaaaaaa tgaccgtaga aatggaaaat ccattaattc
     1141 tattggttga taagaaaatt gataaccttc aagagctgtt gccaattctt gaaaacgtcg
     1201 ctaaatcagg tcgtccatta ttgatcgttg ctgaagatgt tgaaggccaa gcactagcaa
     1261 cattggtagt aaacaacttg cgcggcacat tcaaggttgc agcggttaaa gcccctggtt
     1321 ttggcgatcg tcgtaaagcg atgttgcaag atcttgccat cttgacgggt ggtcaggtta
     1381 tttctgagga gctagggatg tctttagaaa ctgcggatcc ttcttctttg ggtacggcaa
     1441 gcaaggttgt tatcgataaa gaaaacaccg tgattgttga tggcgcaggt actgaagcaa
     1501 gcgttaatac tcgtgttgac cagatccgtg ctgaaatcga aagctcgact tctgattacg
     1561 acatcgaaaa gttacaagaa cgcgttgcta agcttgcggg cggcgttgcc gtgattaagg
     1621 ttggtgcggg ttctgaaatg gaaatgaaag agaagaaaga ccgtgttgac gatgcacttc
     1681 atgcaactcg cgcagcggtt gaagaaggtg ttgttgcggg tggtggtgtt gctttgattc
     1741 gcgcactctc ttcagtaacc gttgttggtg ataacgaaga tcaaaacgtc ggtattgcat
     1801 tggcacttcg tgcgatggaa gctcctatcc gtcaaatcgc gggtaacgca ggtgctgaag
     1861 ggtcagtggt tgttgataaa gtgaaatctg gcacaggtag ctttggtttt aacgccagca
     1921 caggtgagta tggcgatatg attgcgatgg gtattttaga ccctgcaaaa gtcacgcgtt
     1981 catctctaca agccgcggcg tctatcgcag gtttgatgat cacaaccgaa gccatggttg
     2041 cggatgcgcc tgttgaagaa ggcgctggtg gtatgcctga tatgggcggc atgggtggaa
     2101 tgggcggtat gcctggcatg atgtaataa
//