##gff-version 3
##sequence-region BBa_K538310 1 2129
BBa_K538310	.	ribosome_entry_site	462	473	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K538310	.	conserved_region	466	469	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K538310	.	CDS	480	2129	.	+	0	ID=BBa_K538001;Name=BBa_K538001
BBa_K538310	.	start_codon	480	482	.	+	0	ID=annotation2124231;Name=Start Codon;Parent=BBa_K538001
BBa_K538310	.	point_mutation	1387	1387	.	+	0	ID=annotation2128866;Name=A > G (Recode for SapI site);Parent=BBa_K538001
BBa_K538310	.	stop_codon	2124	2129	.	+	0	ID=annotation2124232;Name=Double Stop Codon;Parent=BBa_K538001
BBa_K538310	.	ribosome_entry_site	139	150	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K538310	.	conserved_region	143	146	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K538310	.	promoter	1	130	.	+	0	ID=BBa_K206000;Name=BBa_K206000
BBa_K538310	.	operator	61	78	.	+	0	ID=annotation2049254;Name=AraI2;Parent=BBa_K206000
BBa_K538310	.	promoter	1	131	.	+	0	ID=annotation2049252;Name=promoter;Parent=BBa_K206000
BBa_K538310	.	operator	40	57	.	+	0	ID=annotation2049253;Name=AraI1;Parent=BBa_K206000
BBa_K538310	.	CDS	157	453	.	+	0	ID=BBa_K538000;Name=BBa_K538000
BBa_K538310	.	stop_codon	448	453	.	+	0	ID=annotation2124175;Name=Double Stop Codon;Parent=BBa_K538000
BBa_K538310	.	start_codon	157	159	.	+	0	ID=annotation2124174;Name=Start Codon;Parent=BBa_K538000
>BBa_K538310
acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
gaaaatccgtccattacatgatcgtattgttgttcgccgtaaagaagaagagaccgcaactgcgggtggtattatttta
ccgggcgctgcggcagaaaaaccaaatcaaggtgttgttatctctgtgggtactggccgtattcttgataatggttcag
tgcaagcgctggcggttaacgaaggcgatgttgtcgtttttggtaaatactcaggtcaaaatactatcgatatcgatgg
tgaagaattattgattttgaatgaaagtgatatctacggcgttttagaagcttaataatactagagaaagaggagaaat
actagatggctgctaaagacgtattatttggtgatagcgcacgcgcaaaaatgttggtaggtgtaaacattttagccga
cgcagtaagagttaccttaggacctaaaggtcgtaacgttgttatagaaaaatcatttggtgcaccgatcatcaccaaa
gatggtgtttctgttgcgcgtgaaatcgaattgaaagacaaattcgaaaacatgggcgcacagatggttaaggaagttg
cttctcaagccaacgaccaagccggtgacggcacaacgacagcgactgtactagcacaggcgattatcagcgaaggctt
gaaatctgttgcggctggcatgaatccaatggatcttaaacgtggtattgataaagctacggctgctgttgttgccgcc
attaaagaacaagctcagccttgcttggatacaaaagcaatcgctcaggtagggacaatctctgccaatgccgatgaaa
cggttggtcgtttaattgctgaagcgatggaaaaagtcggtaaagaaggtgtgattaccgttgaagaaggcaaaggcct
tgaagacgagcttgatgttgtagaaggcatgcagttcgatcgcggttacttgtctccgtacttcatcaacaaccaagaa
aaaatgaccgtagaaatggaaaatccattaattctattggttgataagaaaattgataaccttcaagagctgttgccaa
ttcttgaaaacgtcgctaaatcaggtcgtccattattgatcgttgctgaagatgttgaaggccaagcactagcaacatt
ggtagtaaacaacttgcgcggcacattcaaggttgcagcggttaaagcccctggttttggcgatcgtcgtaaagcgatg
ttgcaagatcttgccatcttgacgggtggtcaggttatttctgaggagctagggatgtctttagaaactgcggatcctt
cttctttgggtacggcaagcaaggttgttatcgataaagaaaacaccgtgattgttgatggcgcaggtactgaagcaag
cgttaatactcgtgttgaccagatccgtgctgaaatcgaaagctcgacttctgattacgacatcgaaaagttacaagaa
cgcgttgctaagcttgcgggcggcgttgccgtgattaaggttggtgcgggttctgaaatggaaatgaaagagaagaaag
accgtgttgacgatgcacttcatgcaactcgcgcagcggttgaagaaggtgttgttgcgggtggtggtgttgctttgat
tcgcgcactctcttcagtaaccgttgttggtgataacgaagatcaaaacgtcggtattgcattggcacttcgtgcgatg
gaagctcctatccgtcaaatcgcgggtaacgcaggtgctgaagggtcagtggttgttgataaagtgaaatctggcacag
gtagctttggttttaacgccagcacaggtgagtatggcgatatgattgcgatgggtattttagaccctgcaaaagtcac
gcgttcatctctacaagccgcggcgtctatcgcaggtttgatgatcacaaccgaagccatggttgcggatgcgcctgtt
gaagaaggcgctggtggtatgcctgatatgggcggcatgggtggaatgggcggtatgcctggcatgatgtaataa