LOCUS BBa_K539142 4724 bp DNA linear 05-Nov-2024 DEFINITION crtEBI+RNA thermometer+crtY+terminator ACCESSION BBa_K539142 VERSION BBa_K539142.1 FEATURES Location/Qualifiers RBS 1..4 /label=BBa_K118012 CDS 11..922 /label=BBa_I742151 misc_feature 931..933 /label=SacI RBS 934..940 /label=rbs RBS 931..940 /label=BBa_J15001 CDS 947..1876 /label=crtB coding sequence CDS 947..1879 /label=BBa_K118002 misc_feature 1888..1890 /label=SacI RBS 1891..1897 /label=rbs RBS 1888..1897 /label=BBa_J15001 misc_feature 2381..2386 /label=PstI site removed misc_feature 2792..2797 /label=PstI site removed CDS 1904..3382 /label=crtI coding sequence CDS 1904..3385 /label=BBa_K118003 misc_feature 1..3385 /label=BBa_K274100 RBS 3440..3445 /label=rbs misc_feature 3416..3421 /label=scar adaptation stem_loop 3394..3411 /label=stem_loop stem_loop 3417..3445 /label=predicted stem-loop extending to one position before start codon misc_feature 3394..3445 /label=BBa_K115002 CDS 3452..4600 /label=crtY coding sequence CDS 3452..4603 /label=BBa_K118008 CDS 3452..4600 /label=crtY coding sequence misc_feature 3452..4603 /label=BBa_K118008 CDS 3452..4603 /label=BBa_K539119 terminator 4612..4724 /label=BBa_J61048 misc_feature 3394..4724 /label=BBa_K539138 ORIGIN 1 gaggtactag atgacggtct gcgcaaaaaa acacgttcat ctcactcgcg atgctgcgga 61 gcagttactg gctgatattg atcgacgcct tgatcagtta ttgcccgtgg agggagaacg 121 ggatgttgtg ggtgccgcga tgcgtgaagg tgcgctggca ccgggaaaac gtattcgccc 181 catgttgctg ttgctgaccg cccgcgatct gggttgcgct gtcagccatg acggattact 241 ggatttggcc tgtgcggtgg aaatggtcca cgcggcttcg ctgatccttg acgatatgcc 301 ctgcatggac gatgcgaagc tgcggcgcgg acgccctacc attcattctc attacggaga 361 gcatgtggca atactggcgg cggttgcctt gctgagtaaa gcctttggcg taattgccga 421 tgcagatggc ctcacgccgc tggcaaaaaa tcgggcggtt tctgaactgt caaacgccat 481 cggcatgcaa ggattggttc agggtcagtt caaggatctg tctgaagggg ataagccgcg 541 cagcgctgaa gctattttga tgacgaatca ctttaaaacc agcacgctgt tttgtgcctc 601 catgcagatg gcctcgattg ttgcgaatgc ctccagcgaa gcgcgtgatt gcctgcatcg 661 tttttcactt gatcttggtc aggcatttca actgctggac gatttgaccg atggcatgac 721 cgacaccggt aaggatagca atcaggacgc cggtaaatcg acgctggtca atctgttagg 781 cccgagggcg gttgaagaac gtctgagaca acatcttcag cttgccagtg agcatctctc 841 tgcggcctgc caacacgggc acgccactca acattttatt caggcctggt ttgacaaaaa 901 actcgctgcc gtcagttaat aatactagag ctcaaggagg tactagatga ataatccgtc 961 gttactcaat catgcggtcg aaacgatggc agttggctcg aaaagttttg cgacagcctc 1021 aaagttattt gatgcaaaaa cccggcgcag cgtactgatg ctctacgcct ggtgccgcca 1081 ttgtgacgat gttattgacg atcagacgct gggctttcag gcccggcagc ctgccttaca 1141 aacgcccgaa caacgtctga tgcaacttga gatgaaaacg cgccaggcct atgcaggatc 1201 gcagatgcac gaaccggcgt ttgcggcttt tcaggaagtg gctatggctc atgatatcgc 1261 cccggcttac gcgtttgatc atctggaagg cttcgccatg gatgtacgcg aagcgcaata 1321 cagccaactg gatgatacgc tgcgctattg ctatcacgtt gcaggcgttg tcggcttgat 1381 gatggcgcaa atcatgggcg tgcgggataa cgccacgctg gaccgcgcct gtgaccttgg 1441 gctggcattt cagttgacca atattgctcg cgatattgtg gacgatgcgc atgcgggccg 1501 ctgttatctg ccggcaagct ggctggagca tgaaggtctg aacaaagaga attatgcggc 1561 acctgaaaac cgtcaggcgc tgagccgtat cgcccgtcgt ttggtgcagg aagcagaacc 1621 ttactatttg tctgccacag ccggcctggc agggttgccc ctgcgttccg cctgggcaat 1681 cgctacggcg aagcaggttt accggaaaat aggtgtcaaa gttgaacagg ccggtcagca 1741 agcctgggat cagcggcagt caacgaccac gcccgaaaaa ttaacgctgc tgctggccgc 1801 ctctggtcag gcccttactt cccggatgcg ggctcatcct ccccgccctg cgcatctctg 1861 gcagcgcccg ctctaataat actagagctc aaggaggtac tagatgaaac caactacggt 1921 aattggtgca ggcttcggtg gcctggcact ggcaattcgt ctacaagctg cggggatccc 1981 cgtcttactg cttgaacaac gtgataaacc cggcggtcgg gcttatgtct acgaggatca 2041 ggggtttacc tttgatgcag gcccgacggt tatcaccgat cccagtgcca ttgaagaact 2101 gtttgcactg gcaggaaaac agttaaaaga gtatgtcgaa ctgctgccgg ttacgccgtt 2161 ttaccgcctg tgttgggagt cagggaaggt ctttaattac gataacgatc aaacccggct 2221 cgaagcgcag attcagcagt ttaatccccg cgatgtcgaa ggttatcgtc agtttctgga 2281 ctattcacgc gcggtgttta aagaaggcta tctaaagctc ggtactgtcc cttttttatc 2341 gttcagagac atgcttcgcg ccgcacctca actggcgaaa ctgcaagcat ggagaagcgt 2401 ttacagtaag gttgccagtt acatcgaaga tgaacatctg cgccaggcgt tttctttcca 2461 ctcgctgttg gtgggcggca atcccttcgc cacctcatcc atttatacgt tgatacacgc 2521 gctggagcgt gagtggggcg tctggtttcc gcgtggcggc accggcgcat tagttcaggg 2581 gatgataaag ctgtttcagg atctgggtgg cgaagtcgtg ttaaacgcca gagtcagcca 2641 tatggaaacg acaggaaaca agattgaagc cgtgcattta gaggacggtc gcaggttcct 2701 gacgcaagcc gtcgcgtcaa atgcagatgt ggttcatacc tatcgcgacc tgttaagcca 2761 gcaccctgcc gcggttaagc agtccaacaa actgcaaact aagcgcatga gtaactctct 2821 gtttgtgctc tattttggtt tgaatcacca tcatgatcag ctcgcgcatc acacggtttg 2881 tttcggcccg cgttaccgcg agctgattga cgaaattttt aatcatgatg gcctcgcaga 2941 ggacttctca ctttatctgc acgcgccctg tgtcacggat tcgtcactgg cgcctgaagg 3001 ttgcggcagt tactatgtgt tggcgccggt gccgcattta ggcaccgcga acctcgactg 3061 gacggttgag gggccaaaac tacgcgaccg tatttttgcg taccttgagc agcattacat 3121 gcctggctta cggagtcagc tggtcacgca ccggatgttt acgccgtttg attttcgcga 3181 ccagcttaat gcctatcatg gctcagcctt ttctgtggag cccgttctta cccagagcgc 3241 ctggtttcgg ccgcataacc gcgataaaac cattactaat ctctacctgg tcggcgcagg 3301 cacgcatccc ggcgcaggca ttcctggcgt catcggctcg gcaaaagcga cagcaggttt 3361 gatgctggag gatctgatat aataatacta gagggacaag caatgcttgc cttgaatagt 3421 aacttttgaa tagtgattca ggaggtacta gatgcaaccg cattatgatc tgattctcgt 3481 gggggctgga ctcgcgaatg gccttatcgc cctgcgtctt cagcagcagc aacctgatat 3541 gcgtattttg cttatcgacg ccgcacccca ggcgggcggg aatcatacgt ggtcatttca 3601 ccacgatgat ttgactgaga gccaacatcg ttggatagct ccgctggtgg ttcatcactg 3661 gcccgactat caggtacgct ttcccacacg ccgtcgtaag ctgaacagcg gctacttttg 3721 tattacttct cagcgtttcg ctgaggtttt acagcgacag tttggcccgc acttgtggat 3781 ggataccgcg gtcgcagagg ttaatgcgga atctgttcgg ttgaaaaagg gtcaggttat 3841 cggtgcccgc gcggtgattg acgggcgggg ttatgcggca aattcagcac tgagcgtggg 3901 cttccaggcg tttattggcc aggaatggcg attgagccac ccgcatggtt tatcgtctcc 3961 cattatcatg gatgccacgg tcgatcagca aaatggttat cgcttcgtgt acagcctgcc 4021 gctctcgccg accagattgt taattgaaga cacgcactat attgataatg cgacattaga 4081 tcctgaatgc gcgcggcaaa atatttgcga ctatgccgcg caacagggtt ggcagcttca 4141 gacactgctg cgagaagaac agggcgcctt acccattact ctgtcgggca atgccgacgc 4201 attctggcag cagcgccccc tggcctgtag tggattacgt gccggtctgt tccatcctac 4261 caccggctat tcactgccgc tggcggttgc cgtggccgac cgcctgagtg cacttgatgt 4321 ctttacgtcg gcctcaattc accatgccat tacgcatttt gcccgcgagc gctggcagca 4381 gcagggcttt ttccgcatgc tgaatcgcat gctgttttta gccggacccg ccgattcacg 4441 ctggcgggtt atgcagcgtt tttatggttt acctgaagat ttaattgccc gtttttatgc 4501 gggaaaactc acgctgaccg atcggctacg tattctgagc ggcaagccgc ctgttccggt 4561 attagcagca ttgcaagcca ttatgacgac tcatcgttaa taatactaga gccggcttat 4621 cggtcagttt cacctgattt acgtaaaaac ccgcttcggc gggtttttgc ttttggaggg 4681 gcagaaagat gaatgactgt ccacgacgct atacccaaaa gaaa //