##gff-version 3
##sequence-region BBa_K539142 1 4724
BBa_K539142	.	engineered_region	3394	4724	.	+	0	ID=BBa_K539138;Name=BBa_K539138
BBa_K539142	.	CDS	3452	4603	.	+	0	ID=BBa_K118008;Name=BBa_K118008;Parent=BBa_K539138
BBa_K539142	.	CDS	3452	4600	.	+	0	ID=annotation1995716;Name=crtY coding sequence;Parent=BBa_K118008
BBa_K539142	.	mature_transcript_region	3394	3445	.	+	0	ID=BBa_K115002;Name=BBa_K115002;Parent=BBa_K539138
BBa_K539142	.	stem_loop	3394	3411	.	+	0	ID=annotation1966930;Name=stem_loop;Parent=BBa_K115002
BBa_K539142	.	ribosome_entry_site	3440	3445	.	+	0	ID=annotation1966897;Name=rbs;Parent=BBa_K115002
BBa_K539142	.	sequence_alteration	3416	3421	.	+	0	ID=annotation1966899;Name=scar adaptation;Parent=BBa_K115002
BBa_K539142	.	stem_loop	3417	3445	.	+	0	ID=annotation1966931;Name=predicted stem-loop extending to one position before start codon;Parent=BBa_K115002
BBa_K539142	.	terminator	4612	4724	.	+	0	ID=BBa_J61048;Name=BBa_J61048;Parent=BBa_K539138
BBa_K539142	.	CDS	3452	4603	.	+	0	ID=BBa_K539119;Name=BBa_K539119;Parent=BBa_K539138
BBa_K539142	.	CDS	3452	4600	.	+	0	ID=annotation2131423;Name=crtY coding sequence;Parent=BBa_K539119
BBa_K539142	.	engineered_region	3452	4603	.	+	0	ID=annotation2131424;Name=BBa_K118008;Parent=BBa_K539119
BBa_K539142	.	engineered_region	1	3385	.	+	0	ID=BBa_K274100;Name=BBa_K274100
BBa_K539142	.	ribosome_entry_site	1	4	.	+	0	ID=BBa_K118012;Name=BBa_K118012;Parent=BBa_K274100
BBa_K539142	.	CDS	947	1879	.	+	0	ID=BBa_K118002;Name=BBa_K118002;Parent=BBa_K274100
BBa_K539142	.	CDS	947	1876	.	+	0	ID=annotation1995701;Name=crtB coding sequence;Parent=BBa_K118002
BBa_K539142	.	ribosome_entry_site	931	940	.	+	0	ID=BBa_J15001;Name=BBa_J15001;Parent=BBa_K274100
BBa_K539142	.	sequence_feature	931	933	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_K539142	.	ribosome_entry_site	934	940	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
BBa_K539142	.	CDS	11	922	.	+	0	ID=BBa_I742151;Name=BBa_I742151;Parent=BBa_K274100
BBa_K539142	.	CDS	1904	3385	.	+	0	ID=BBa_K118003;Name=BBa_K118003;Parent=BBa_K274100
BBa_K539142	.	point_mutation	2792	2797	.	+	0	ID=annotation1968245;Name=PstI site removed;Parent=BBa_K118003
BBa_K539142	.	point_mutation	2381	2386	.	+	0	ID=annotation1968244;Name=PstI site removed;Parent=BBa_K118003
BBa_K539142	.	CDS	1904	3382	.	+	0	ID=annotation1995702;Name=crtI coding sequence;Parent=BBa_K118003
BBa_K539142	.	ribosome_entry_site	1888	1897	.	+	0	ID=BBa_J15001;Name=BBa_J15001;Parent=BBa_K274100
BBa_K539142	.	sequence_feature	1888	1890	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_K539142	.	ribosome_entry_site	1891	1897	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
>BBa_K539142
gaggtactagatgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatatt
gatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctgg
caccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggatt
actggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcg
aagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctga
gtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaa
cgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagct
attttgatgacgaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcct
ccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccga
tggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcg
gttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactc
aacattttattcaggcctggtttgacaaaaaactcgctgccgtcagttaataatactagagctcaaggaggtactagat
gaataatccgtcgttactcaatcatgcggtcgaaacgatggcagttggctcgaaaagttttgcgacagcctcaaagtta
tttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcaga
cgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgcca
ggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccg
gcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgc
gctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtgcgggataacgccacgctgga
ccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgc
tgttatctgccggcaagctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgc
tgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggttgcc
cctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcag
caagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgctgctgctggccgcctctggtcaggccctta
cttcccggatgcgggctcatcctccccgccctgcgcatctctggcagcgcccgctctaataatactagagctcaaggag
gtactagatgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcgggg
atccccgtcttactgcttgaacaacgtgataaacccggcggtcgggcttatgtctacgaggatcaggggtttacctttg
atgcaggcccgacggttatcaccgatcccagtgccattgaagaactgtttgcactggcaggaaaacagttaaaagagta
tgtcgaactgctgccggttacgccgttttaccgcctgtgttgggagtcagggaaggtctttaattacgataacgatcaa
acccggctcgaagcgcagattcagcagtttaatccccgcgatgtcgaaggttatcgtcagtttctggactattcacgcg
cggtgtttaaagaaggctatctaaagctcggtactgtcccttttttatcgttcagagacatgcttcgcgccgcacctca
actggcgaaactgcaagcatggagaagcgtttacagtaaggttgccagttacatcgaagatgaacatctgcgccaggcg
ttttctttccactcgctgttggtgggcggcaatcccttcgccacctcatccatttatacgttgatacacgcgctggagc
gtgagtggggcgtctggtttccgcgtggcggcaccggcgcattagttcaggggatgataaagctgtttcaggatctggg
tggcgaagtcgtgttaaacgccagagtcagccatatggaaacgacaggaaacaagattgaagccgtgcatttagaggac
ggtcgcaggttcctgacgcaagccgtcgcgtcaaatgcagatgtggttcatacctatcgcgacctgttaagccagcacc
ctgccgcggttaagcagtccaacaaactgcaaactaagcgcatgagtaactctctgtttgtgctctattttggtttgaa
tcaccatcatgatcagctcgcgcatcacacggtttgtttcggcccgcgttaccgcgagctgattgacgaaatttttaat
catgatggcctcgcagaggacttctcactttatctgcacgcgccctgtgtcacggattcgtcactggcgcctgaaggtt
gcggcagttactatgtgttggcgccggtgccgcatttaggcaccgcgaacctcgactggacggttgaggggccaaaact
acgcgaccgtatttttgcgtaccttgagcagcattacatgcctggcttacggagtcagctggtcacgcaccggatgttt
acgccgtttgattttcgcgaccagcttaatgcctatcatggctcagccttttctgtggagcccgttcttacccagagcg
cctggtttcggccgcataaccgcgataaaaccattactaatctctacctggtcggcgcaggcacgcatcccggcgcagg
cattcctggcgtcatcggctcggcaaaagcgacagcaggtttgatgctggaggatctgatataataatactagagggac
aagcaatgcttgccttgaatagtaacttttgaatagtgattcaggaggtactagatgcaaccgcattatgatctgattc
tcgtgggggctggactcgcgaatggccttatcgccctgcgtcttcagcagcagcaacctgatatgcgtattttgcttat
cgacgccgcaccccaggcgggcgggaatcatacgtggtcatttcaccacgatgatttgactgagagccaacatcgttgg
atagctccgctggtggttcatcactggcccgactatcaggtacgctttcccacacgccgtcgtaagctgaacagcggct
acttttgtattacttctcagcgtttcgctgaggttttacagcgacagtttggcccgcacttgtggatggataccgcggt
cgcagaggttaatgcggaatctgttcggttgaaaaagggtcaggttatcggtgcccgcgcggtgattgacgggcggggt
tatgcggcaaattcagcactgagcgtgggcttccaggcgtttattggccaggaatggcgattgagccacccgcatggtt
tatcgtctcccattatcatggatgccacggtcgatcagcaaaatggttatcgcttcgtgtacagcctgccgctctcgcc
gaccagattgttaattgaagacacgcactatattgataatgcgacattagatcctgaatgcgcgcggcaaaatatttgc
gactatgccgcgcaacagggttggcagcttcagacactgctgcgagaagaacagggcgccttacccattactctgtcgg
gcaatgccgacgcattctggcagcagcgccccctggcctgtagtggattacgtgccggtctgttccatcctaccaccgg
ctattcactgccgctggcggttgccgtggccgaccgcctgagtgcacttgatgtctttacgtcggcctcaattcaccat
gccattacgcattttgcccgcgagcgctggcagcagcagggctttttccgcatgctgaatcgcatgctgtttttagccg
gacccgccgattcacgctggcgggttatgcagcgtttttatggtttacctgaagatttaattgcccgtttttatgcggg
aaaactcacgctgaccgatcggctacgtattctgagcggcaagccgcctgttccggtattagcagcattgcaagccatt
atgacgactcatcgttaataatactagagccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcg
ggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa