BBa_K539142
type: ComponentDefinition
persistent identity: BBa K539142
display id: BBa_K539142
version: 1
was generated by: iGEM to SBOL conversion
description: crtEBI+RNA thermometer+crtY+terminator
created: 2011-09-18T11:00:00Z
modified: 2015-05-08T01:12:37Z
mutable provenance: CrtEBI gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087), a type of Gram negative Enterobacteriaceae. This composite biobrick come from BBa_K274100. CrtY comes from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). RNA thermometer comes from Salmonella Enterica Tyhpy (NC_010102)).
owned by: james
owned by: myers
mutable description: CrtEBI with RBS that is assembled by previous team is created by standard assembly of Part BBa_K118014 (CrtE with rbs), Part BBa_K118006 (CrtB with rbs) and Part BBa_K118005 (CrtI with rbs), which are submitted by previous iGEM teams. Together, enzymes CrtE, CrtB and CrtI convert colourless farnesyl pyrophosphate to red lycopene (via intermediates geranylgeranyl pyroiphosphate and phytoene). And then crtY(lycopene B-cyclase) converts lycopene to orange beta-carotene. The RNA thermometer that can be used for temperature sensitive post-transcriptional regulation which is based on a RNA thermometer from Salmonella Enterica Tyhpy initiates translation at 37??C. Therefore crtY expresses significantly at 37??C. The native terminator is included.
discontinued: false
dominant: false
experience: experience/Works
group u list: _705_
m user id: 0
owner id: 10230
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: We design this composite biobrick to show red and orange pigment at different temperature. Lower than 37??C the pigment is red lycopene and at 37??C the pigment is orange.
star: false
creator: Yang, Yi-Pei
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K539138
component: BBa_K274100
sequence annotation: BBa_K274100
sequence annotation: BBa_K539138
sequence: https://synbiohub.org/public/igem/BBa_K539142_sequence/1

BBa_K539138
type: Component
persistent identity: component2133804
display id: component2133804
version: 1
top level: BBa_K539142
definition: BBa_K539138
access: public

BBa_K274100
type: Component
persistent identity: component2133794
display id: component2133794
version: 1
top level: BBa_K539142
definition: BBa_K274100
access: public

BBa_K274100
type: SequenceAnnotation
persistent identity: annotation2133794
display id: annotation2133794
version: 1
top level: BBa_K539142
location: https://synbiohub.org/public/igem/BBa_K539142/annotation2133794/range2133794/1
component: BBa_K274100

https://synbiohub.org/public/igem/BBa_K539142/annotation2133794/range2133794/1
type: Range
persistent identity: range2133794
display id: range2133794
version: 1
top level: BBa_K539142
direction: direction/reverse
start: 1
end: 3385
orientation: inline

BBa_K539138
type: SequenceAnnotation
persistent identity: annotation2133804
display id: annotation2133804
version: 1
top level: BBa_K539142
location: https://synbiohub.org/public/igem/BBa_K539142/annotation2133804/range2133804/1
component: BBa_K539138

https://synbiohub.org/public/igem/BBa_K539142/annotation2133804/range2133804/1
type: Range
persistent identity: range2133804
display id: range2133804
version: 1
top level: BBa_K539142
direction: direction/reverse
start: 3394
end: 4724
orientation: inline

BBa_K539138
type: ComponentDefinition
persistent identity: BBa K539138
display id: BBa_K539138
version: 1
was generated by: iGEM to SBOL conversion
description: crtY (lycopene cyclase) with RNA thermometer and terminator
created: 2011-09-17T11:00:00Z
modified: 2015-05-08T01:12:37Z
mutable provenance: RNA thermometer is taken from Salmonella Enterica Tyhpy (NC_010102)). The coding sequence of crtY come from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087)
owned by: james
owned by: myers
mutable description: This is the coding sequence of crtY from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes lycopene B-cyclase, part of the carotenoid biosynthesis pathway, which converts lycopene to B-carotene. RNA thermometer is preceded with crtY. The RNA thermometer that can be used for temperature sensitive post-transcriptional regulation which is based on a RNA thermometer from Salmonella Enterica Tyhpy initiates translation at 37??C. Native terminator is included in the part.
discontinued: false
dominant: false
experience: experience/Works
group u list: _705_
m user id: 0
owner id: 10230
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: CrtY expresses more quantities in 37 degree centigrade because of RNA thermometer.
star: false
creator: Yang, Yi-Pei
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K539119
component: BBa_K118008
component: BBa_K115002
component: BBa_J61048
sequence annotation: BBa_K115002
sequence annotation: BBa_K118008
sequence annotation: BBa_K539119
sequence annotation: BBa_J61048
sequence: https://synbiohub.org/public/igem/BBa_K539138_sequence/1

BBa_K539119
type: Component
persistent identity: component2132495
display id: component2132495
version: 1
top level: BBa_K539138
definition: BBa_K539119
access: public

BBa_K118008
type: Component
persistent identity: component2132494
display id: component2132494
version: 1
top level: BBa_K539138
definition: BBa_K118008
access: public

BBa_K115002
type: Component
persistent identity: component2132492
display id: component2132492
version: 1
top level: BBa_K539138
definition: BBa_K115002
access: public

BBa_J61048
type: Component
persistent identity: component2132496
display id: component2132496
version: 1
top level: BBa_K539138
definition: BBa_J61048
access: public

BBa_K115002
type: SequenceAnnotation
persistent identity: annotation2132492
display id: annotation2132492
version: 1
top level: BBa_K539138
location: https://synbiohub.org/public/igem/BBa_K539138/annotation2132492/range2132492/1
component: BBa_K115002

https://synbiohub.org/public/igem/BBa_K539138/annotation2132492/range2132492/1
type: Range
persistent identity: range2132492
display id: range2132492
version: 1
top level: BBa_K539138
direction: direction/reverse
start: 1
end: 52
orientation: inline

BBa_K118008
type: SequenceAnnotation
persistent identity: annotation2132494
display id: annotation2132494
version: 1
top level: BBa_K539138
location: https://synbiohub.org/public/igem/BBa_K539138/annotation2132494/range2132494/1
component: BBa_K118008

https://synbiohub.org/public/igem/BBa_K539138/annotation2132494/range2132494/1
type: Range
persistent identity: range2132494
display id: range2132494
version: 1
top level: BBa_K539138
direction: direction/reverse
start: 59
end: 1210
orientation: inline

BBa_K539119
type: SequenceAnnotation
persistent identity: annotation2132495
display id: annotation2132495
version: 1
top level: BBa_K539138
location: https://synbiohub.org/public/igem/BBa_K539138/annotation2132495/range2132495/1
component: BBa_K539119

https://synbiohub.org/public/igem/BBa_K539138/annotation2132495/range2132495/1
type: Range
persistent identity: range2132495
display id: range2132495
version: 1
top level: BBa_K539138
direction: direction/reverse
start: 59
end: 1210
orientation: inline

BBa_J61048
type: SequenceAnnotation
persistent identity: annotation2132496
display id: annotation2132496
version: 1
top level: BBa_K539138
location: https://synbiohub.org/public/igem/BBa_K539138/annotation2132496/range2132496/1
component: BBa_J61048

https://synbiohub.org/public/igem/BBa_K539138/annotation2132496/range2132496/1
type: Range
persistent identity: range2132496
display id: range2132496
version: 1
top level: BBa_K539138
direction: direction/reverse
start: 1219
end: 1331
orientation: inline

BBa_K274100
type: ComponentDefinition
persistent identity: BBa K274100
display id: BBa_K274100
version: 1
was generated by: iGEM to SBOL conversion
description: CrtEBI with rbs
created: 2009-09-17T11:00:00Z
modified: 2015-05-08T01:11:45Z
mutable provenance: Gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087), a type of Gram negative Enterobacteriaceae. This Composite Biobrick is created by standard assembly of Part BBa_K118014, Part BBa_K118006 and Part BBa_K118005.
owned by: james
owned by: myers
mutable description: This Composite Biobrick is created by standard assembly of Part BBa_K118014 (CrtE with rbs), Part BBa_K118006 (CrtB with rbs) and Part BBa_K118005 (CrtI with rbs), which are submitted by previous iGEM teams. The whole cassette is on plasmid pSB1A2 (high copy, Ampicillin resistance). Together, enzymes CrtE, CrtB and CrtI convert colourless farnesyl pyrophosphate to red lycopene (via intermediates geranylgeranyl pyroiphosphate and phytoene). The red lycopene pigment can then be used as a coloured signal output, e.g. for biosensors. There is already individual ribosome binding site before each enzyme gene sequence. Internal restriction sites have been removed by previous iGEM teams. Please refer to Parts BBa_K118014, BBa_K118006 and BBa_K118005 for more information on individual Biobricks components. This Composite Biobrick has been put under constitutive promoter R0011 (see Part BBa_K274110) and arabinose-inducible promoter I0500 (see Part BBa_274120), transformed and tested in E.coli strain MG1655. Amount of lycopene produced can be measured by photospectrometer with absorbance at 475nm (lycopene extraction using acetone).
discontinued: false
dominant: true
experience: experience/Works
group u list: _374_
m user id: 0
owner id: 5416
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The colourless precursor, farnesyl pyrophosphate, is naturally produced in E.coli using Non-mevalonate Pathway (from pyruvate and glyceraldehyde-3-phosphate). Our experiences showed that the Composite Biobrick, when put under a promoter, works better in E.coli strains with up-regulated level of precursors. E.coli strain MG1655 demonostrates higher level of pigment (lycopene) production than strain TOP10.
star: false
creator: Siming Ma
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K118003
component: BBa_J15001
component: BBa_I742151
component: BBa_K118012
component: BBa_K118002
sequence annotation: BBa_K118012
sequence annotation: BBa_I742151
sequence annotation: BBa_J15001
sequence annotation: BBa_K118002
sequence annotation: BBa_K118003
sequence: https://synbiohub.org/public/igem/BBa_K274100_sequence/1

BBa_K118003
type: Component
persistent identity: component2021361
display id: component2021361
version: 1
top level: BBa_K274100
definition: BBa_K118003
access: public

BBa_J15001
type: Component
persistent identity: component2021357
display id: component2021357
version: 1
top level: BBa_K274100
definition: BBa_J15001
access: public

BBa_I742151
type: Component
persistent identity: component2021349
display id: component2021349
version: 1
top level: BBa_K274100
definition: BBa_I742151
access: public

BBa_K118012
type: Component
persistent identity: component2021348
display id: component2021348
version: 1
top level: BBa_K274100
definition: BBa_K118012
access: public

BBa_K118002
type: Component
persistent identity: component2021354
display id: component2021354
version: 1
top level: BBa_K274100
definition: BBa_K118002
access: public

BBa_J15001
type: Component
persistent identity: component2021352
display id: component2021352
version: 1
top level: BBa_K274100
definition: BBa_J15001
access: public

BBa_K118012
type: SequenceAnnotation
persistent identity: annotation2021348
display id: annotation2021348
version: 1
top level: BBa_K274100
location: https://synbiohub.org/public/igem/BBa_K274100/annotation2021348/range2021348/1
component: BBa_K118012

https://synbiohub.org/public/igem/BBa_K274100/annotation2021348/range2021348/1
type: Range
persistent identity: range2021348
display id: range2021348
version: 1
top level: BBa_K274100
direction: direction/reverse
start: 1
end: 4
orientation: inline

BBa_I742151
type: SequenceAnnotation
persistent identity: annotation2021349
display id: annotation2021349
version: 1
top level: BBa_K274100
location: https://synbiohub.org/public/igem/BBa_K274100/annotation2021349/range2021349/1
component: BBa_I742151

https://synbiohub.org/public/igem/BBa_K274100/annotation2021349/range2021349/1
type: Range
persistent identity: range2021349
display id: range2021349
version: 1
top level: BBa_K274100
direction: direction/reverse
start: 11
end: 922
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2021352
display id: annotation2021352
version: 1
top level: BBa_K274100
location: https://synbiohub.org/public/igem/BBa_K274100/annotation2021352/range2021352/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K274100/annotation2021352/range2021352/1
type: Range
persistent identity: range2021352
display id: range2021352
version: 1
top level: BBa_K274100
direction: direction/reverse
start: 931
end: 940
orientation: inline

BBa_K118002
type: SequenceAnnotation
persistent identity: annotation2021354
display id: annotation2021354
version: 1
top level: BBa_K274100
location: https://synbiohub.org/public/igem/BBa_K274100/annotation2021354/range2021354/1
component: BBa_K118002

https://synbiohub.org/public/igem/BBa_K274100/annotation2021354/range2021354/1
type: Range
persistent identity: range2021354
display id: range2021354
version: 1
top level: BBa_K274100
direction: direction/reverse
start: 947
end: 1879
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2021357
display id: annotation2021357
version: 1
top level: BBa_K274100
location: https://synbiohub.org/public/igem/BBa_K274100/annotation2021357/range2021357/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K274100/annotation2021357/range2021357/1
type: Range
persistent identity: range2021357
display id: range2021357
version: 1
top level: BBa_K274100
direction: direction/reverse
start: 1888
end: 1897
orientation: inline

BBa_K118003
type: SequenceAnnotation
persistent identity: annotation2021361
display id: annotation2021361
version: 1
top level: BBa_K274100
location: https://synbiohub.org/public/igem/BBa_K274100/annotation2021361/range2021361/1
component: BBa_K118003

https://synbiohub.org/public/igem/BBa_K274100/annotation2021361/range2021361/1
type: Range
persistent identity: range2021361
display id: range2021361
version: 1
top level: BBa_K274100
direction: direction/reverse
start: 1904
end: 3385
orientation: inline

BBa_J15001
type: ComponentDefinition
persistent identity: BBa J15001
display id: BBa_J15001
version: 1
was generated by: iGEM to SBOL conversion
description: strong synthetic E. coli ribosome binding site with SacI site.
created: 2007-07-12T11:00:00Z
modified: 2015-08-31T04:08:32Z
mutable provenance: Synthetic.
owned by: james
owned by: myers
mutable description: This is a strong synthetic E. coli ribosome binding site. It is synthesised as two complementary oligonucleotides rather than being incorporated into a biobrick plasmid. It incorporates a SacI site overlapping the XbaI site, which is preserved when it is added to any other biobrick. This allows easy detection of the RBS after it has been added upstream of a biobrick coding sequence in a plasmid vector.
discontinued: false
dominant: false
experience: experience/None
group u list: _163_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Note the presence of a SacI site overlapping the XbaI site, which is preserved when this biobrick is added to any other biobrick. At the time of writing, this biobrick is added as a short piece of DNA composed of two complementary oligonucleotides rather than being incorporated into a biobrick cloning vector by itself. It can be added upstream of a biobrick coding sequence, and its presence can easily be detected in miniprep DNA on a gel by using a SacI-SpeI or similar digest.
star: false
creator: Chris French
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: SacI
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_J15001_sequence/1

SacI
type: SequenceAnnotation
persistent identity: annotation1938045
display id: annotation1938045
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
type: Range
persistent identity: range1938045
display id: range1938045
version: 1
top level: BBa_J15001
direction: direction/forward
start: 1
end: 3
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1938046
display id: annotation1938046
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
type: Range
persistent identity: range1938046
display id: range1938046
version: 1
top level: BBa_J15001
direction: direction/forward
start: 4
end: 10
orientation: inline

BBa_I742151
type: ComponentDefinition
persistent identity: BBa I742151
display id: BBa_I742151
version: 1
was generated by: iGEM to SBOL conversion
description: crtE (geranylgeranyl pyrophosphate synthase) coding sequence.
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
mutable description: crtE (geranylgeranyl pyrophosphate synthase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Part of the carotenoid biosynthesis parthway.
discontinued: false
dominant: false
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No important considerations.
star: false
creator: Chris French
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_I742151_sequence/1

BBa_K115002
type: ComponentDefinition
persistent identity: BBa K115002
display id: BBa_K115002
version: 1
was generated by: iGEM to SBOL conversion
description: RNA thermometer (FourU)
created: 2008-07-14T11:00:00Z
modified: 2015-05-08T01:09:27Z
mutable provenance: Salmonella Enterica Tyhpy (CP000886.1)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Thermo sensitive small RNA (ROSE structure) which can be used as a RNA regulator.
discontinued: false
dominant: false
experience: experience/None
group u list: _223_
m user id: 0
owner id: 3006
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Part of the sequence is altered because of the scar...
star: true
creator: Bastiaan van den Berg
type: DnaRegion
role: SO:0000834
role: partType/RNA
sequence annotation: stem_loop
sequence annotation: scar adaptation
sequence annotation: predicted stem-loop extending to one position before start codon
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K115002_sequence/1

stem_loop
type: SequenceAnnotation
persistent identity: annotation1966930
display id: annotation1966930
version: 1
top level: BBa_K115002
location: https://synbiohub.org/public/igem/BBa_K115002/annotation1966930/range1966930/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_K115002/annotation1966930/range1966930/1
type: Range
persistent identity: range1966930
display id: range1966930
version: 1
top level: BBa_K115002
direction: direction/forward
start: 1
end: 18
orientation: inline

scar adaptation
type: SequenceAnnotation
persistent identity: annotation1966899
display id: annotation1966899
version: 1
top level: BBa_K115002
location: https://synbiohub.org/public/igem/BBa_K115002/annotation1966899/range1966899/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_K115002/annotation1966899/range1966899/1
type: Range
persistent identity: range1966899
display id: range1966899
version: 1
top level: BBa_K115002
direction: direction/forward
start: 23
end: 28
orientation: inline

predicted stem-loop extending to one position before start codon
type: SequenceAnnotation
persistent identity: annotation1966931
display id: annotation1966931
version: 1
top level: BBa_K115002
location: https://synbiohub.org/public/igem/BBa_K115002/annotation1966931/range1966931/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_K115002/annotation1966931/range1966931/1
type: Range
persistent identity: range1966931
display id: range1966931
version: 1
top level: BBa_K115002
direction: direction/forward
start: 24
end: 52
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1966897
display id: annotation1966897
version: 1
top level: BBa_K115002
location: https://synbiohub.org/public/igem/BBa_K115002/annotation1966897/range1966897/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K115002/annotation1966897/range1966897/1
type: Range
persistent identity: range1966897
display id: range1966897
version: 1
top level: BBa_K115002
direction: direction/forward
start: 47
end: 52
orientation: inline

BBa_K118002
type: ComponentDefinition
persistent identity: BBa K118002
display id: BBa_K118002
version: 1
was generated by: iGEM to SBOL conversion
description: crtB coding sequence encoding phytoene synthase
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the coding sequence of crtB from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes phytoene synthase, part of the carotenoid biosynthesis pathway, which converts geranylgeranyl diphosphate to phytoene. Reference: Misawa,N., Nakagawa,M., Kobayashi,K., Yamano,S., Izawa,Y., Nakamura,K. and Harashima,K. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. J. Bacteriol. 172 (12), 6704-6712 (1990)
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: No special considerations.
star: true
creator: Andrew Hall
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: crtB coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118002_sequence/1

crtB coding sequence
type: SequenceAnnotation
persistent identity: annotation1995701
display id: annotation1995701
version: 1
top level: BBa_K118002
location: https://synbiohub.org/public/igem/BBa_K118002/annotation1995701/range1995701/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K118002/annotation1995701/range1995701/1
type: Range
persistent identity: range1995701
display id: range1995701
version: 1
top level: BBa_K118002
direction: direction/forward
start: 1
end: 930
orientation: inline

BBa_K118003
type: ComponentDefinition
persistent identity: BBa K118003
display id: BBa_K118003
version: 1
was generated by: iGEM to SBOL conversion
description: crtI coding sequence encoding phytoene dehydrogenase
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the coding sequence of crtI from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes phytoene dehydrogenase, part of the carotenoid biosynthesis pathway, which converts Phytoene to Lycopene.
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The native crtI sequence contains two internal PstI sites. Primers crtImutf1 and crtImutf2 were ordered so that the MABEL protocol (invented for this purpose) could be used to remove these sites. The mutagenic primers were as follows: * crtImutf1: gtt tac agt aag gtt gcc * crtImutf2: agt aac tct ctg ttt gtg c In both cases, the PstI site CTGCAG was converted to CTGCAA, a silent mutation.
star: true
creator: Andrew Hall
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: crtI coding sequence
sequence annotation: PstI site removed
sequence: https://synbiohub.org/public/igem/BBa_K118003_sequence/1

crtI coding sequence
type: SequenceAnnotation
persistent identity: annotation1995702
display id: annotation1995702
version: 1
top level: BBa_K118003
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1995702/range1995702/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K118003/annotation1995702/range1995702/1
type: Range
persistent identity: range1995702
display id: range1995702
version: 1
top level: BBa_K118003
direction: direction/forward
start: 1
end: 1479
orientation: inline

PstI site removed
type: SequenceAnnotation
persistent identity: annotation1968244
display id: annotation1968244
version: 1
top level: BBa_K118003
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1968244/range1968244/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K118003/annotation1968244/range1968244/1
type: Range
persistent identity: range1968244
display id: range1968244
version: 1
top level: BBa_K118003
direction: direction/forward
start: 478
end: 483
orientation: inline

PstI site removed
type: SequenceAnnotation
persistent identity: annotation1968245
display id: annotation1968245
version: 1
top level: BBa_K118003
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1968245/range1968245/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K118003/annotation1968245/range1968245/1
type: Range
persistent identity: range1968245
display id: range1968245
version: 1
top level: BBa_K118003
direction: direction/forward
start: 889
end: 894
orientation: inline

BBa_K118008
type: ComponentDefinition
persistent identity: BBa K118008
display id: BBa_K118008
version: 1
was generated by: iGEM to SBOL conversion
description: crtY coding sequence encoding lycopene B-cyclase
created: 2008-08-18T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the coding sequence of crtY from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes lycopene B-cyclase, part of the carotenoid biosynthesis pathway, which converts lycopene to B-carotene.
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: No special considerations
star: true
creator: Andrew Hall
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: crtY coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118008_sequence/1

crtY coding sequence
type: SequenceAnnotation
persistent identity: annotation1995716
display id: annotation1995716
version: 1
top level: BBa_K118008
location: https://synbiohub.org/public/igem/BBa_K118008/annotation1995716/range1995716/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K118008/annotation1995716/range1995716/1
type: Range
persistent identity: range1995716
display id: range1995716
version: 1
top level: BBa_K118008
direction: direction/forward
start: 1
end: 1149
orientation: inline

BBa_K118012
type: ComponentDefinition
persistent identity: BBa K118012
display id: BBa_K118012
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic ribosome binding site added by Son of Babel procedure
created: 2008-08-18T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
mutable description: This is a 4bp synthetic ribosome binding site which can be added by fusion PCR using a reverse primer and the Son of Babel cloning system.
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The part can be added by fusion PCR using a Son of Babel reverse primer.
star: false
creator: Andrew Hall
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_K118012_sequence/1

BBa_K539119
type: ComponentDefinition
persistent identity: BBa K539119
display id: BBa_K539119
version: 1
was generated by: iGEM to SBOL conversion
description: crtY (lycopene cyclase)
created: 2011-09-16T11:00:00Z
modified: 2015-05-08T01:12:37Z
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: crtY (lycopene cyclase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. The native ribosome binding site is included. This is part of the carotenoid biosynthesis pathway, converting lycopene to beta-carotene.
discontinued: false
dominant: false
experience: experience/Works
group u list: _705_
m user id: 0
owner id: 10230
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: This part is from previous team, but we find a point mutation in the sequence. We have correct the mutation, and this is still an available biobrick.
star: false
creator: Yang, Yi-Pei
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_K118008
sequence annotation: crtY coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K539119_sequence/1

BBa_K118008
type: SequenceAnnotation
persistent identity: annotation2131424
display id: annotation2131424
version: 1
top level: BBa_K539119
location: https://synbiohub.org/public/igem/BBa_K539119/annotation2131424/range2131424/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_K539119/annotation2131424/range2131424/1
type: Range
persistent identity: range2131424
display id: range2131424
version: 1
top level: BBa_K539119
direction: direction/reverse
start: 1
end: 1152
orientation: inline

crtY coding sequence
type: SequenceAnnotation
persistent identity: annotation2131423
display id: annotation2131423
version: 1
top level: BBa_K539119
location: https://synbiohub.org/public/igem/BBa_K539119/annotation2131423/range2131423/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K539119/annotation2131423/range2131423/1
type: Range
persistent identity: range2131423
display id: range2131423
version: 1
top level: BBa_K539119
direction: direction/forward
start: 1
end: 1149
orientation: inline

BBa_J61048
type: ComponentDefinition
persistent identity: BBa J61048
display id: BBa_J61048
version: 1
was generated by: iGEM to SBOL conversion
description: [rnpB-T1] Terminator
created: 2007-02-20T12:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: bob
owned by: james
owned by: myers
mutable description: bob
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: bob
star: true
creator: John Anderson
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_J61048_sequence/1

https://synbiohub.org/public/igem/BBa_K539142_sequence/1
type: Sequence
persistent identity: BBa K539142 sequence
display id: BBa_K539142_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaggtactagatgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggcctggtttgacaaaaaactcgctgccgtcagttaataatactagagctcaaggaggtactagatgaataatccgtcgttactcaatcatgcggtcgaaacgatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtgcgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgcatctctggcagcgcccgctctaataatactagagctcaaggaggtactagatgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K539138_sequence/1
type: Sequence
persistent identity: BBa K539138 sequence
display id: BBa_K539138_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggacaagcaatgcttgccttgaatagtaacttttgaatagtgattcaggaggtactagatgcaaccgcattatgatctgattctcgtgggggctggactcgcgaatggccttatcgccctgcgtcttcagcagcagcaacctgatatgcgtattttgcttatcgacgccgcaccccaggcgggcgggaatcatacgtggtcatttcaccacgatgatttgactgagagccaacatcgttggatagctccgctggtggttcatcactggcccgactatcaggtacgctttcccacacgccgtcgtaagctgaacagcggctacttttgtattacttctcagcgtttcgctgaggttttacagcgacagtttggcccgcacttgtggatggataccgcggtcgcagaggttaatgcggaatctgttcggttgaaaaagggtcaggttatcggtgcccgcgcggtgattgacgggcggggttatgcggcaaattcagcactgagcgtgggcttccaggcgtttattggccaggaatggcgattgagccacccgcatggtttatcgtctcccattatcatggatgccacggtcgatcagcaaaatggttatcgcttcgtgtacagcctgccgctctcgccgaccagattgttaattgaagacacgcactatattgataatgcgacattagatcctgaatgcgcgcggcaaaatatttgcgactatgccgcgcaacagggttggcagcttcagacactgctgcgagaagaacagggcgccttacccattactctgtcgggcaatgccgacgcattctggcagcagcgccccctggcctgtagtggattacgtgccggtctgttccatcctaccaccggctattcactgccgctggcggttgccgtggccgaccgcctgagtgcacttgatgtctttacgtcggcctcaattcaccatgccattacgcattttgcccgcgagcgctggcagcagcagggctttttccgcatgctgaatcgcatgctgtttttagccggacccgccgattcacgctggcgggttatgcagcgtttttatggtttacctgaagatttaattgcccgtttttatgcgggaaaactcacgctgaccgatcggctacgtattctgagcggcaagccgcctgttccggtattagcagcattgcaagccattatgacgactcatcgttaataatactagagccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K274100_sequence/1
type: Sequence
persistent identity: BBa K274100 sequence
display id: BBa_K274100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaggtactagatgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggcctggtttgacaaaaaactcgctgccgtcagttaataatactagagctcaaggaggtactagatgaataatccgtcgttactcaatcatgcggtcgaaacgatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtgcgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgcatctctggcagcgcccgctctaataatactagagctcaaggaggtactagatgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J15001_sequence/1
type: Sequence
persistent identity: BBa J15001 sequence
display id: BBa_J15001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcaaggagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742151_sequence/1
type: Sequence
persistent identity: BBa I742151 sequence
display id: BBa_I742151_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggcctggtttgacaaaaaactcgctgccgtcagttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K115002_sequence/1
type: Sequence
persistent identity: BBa K115002 sequence
display id: BBa_K115002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggacaagcaatgcttgccttgaatagtaacttttgaatagtgattcaggagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118002_sequence/1
type: Sequence
persistent identity: BBa K118002 sequence
display id: BBa_K118002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaataatccgtcgttactcaatcatgcggtcgaaacgatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtgcgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgcatctctggcagcgcccgctctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118003_sequence/1
type: Sequence
persistent identity: BBa K118003 sequence
display id: BBa_K118003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaacgtgataaacccggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcaggcccgacggttatcaccgatcccagtgccattgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgctgccggttacgccgttttaccgcctgtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggctcgaagcgcagattcagcagtttaatccccgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtttaaagaaggctatctaaagctcggtactgtcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcgaaactgcaagcatggagaagcgtttacagtaaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctttccactcgctgttggtgggcggcaatcccttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtggggcgtctggtttccgcgtggcggcaccggcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaagtcgtgttaaacgccagagtcagccatatggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgcaggttcctgacgcaagccgtcgcgtcaaatgcagatgtggttcatacctatcgcgacctgttaagccagcaccctgccgcggttaagcagtccaacaaactgcaaactaagcgcatgagtaactctctgtttgtgctctattttggtttgaatcaccatcatgatcagctcgcgcatcacacggtttgtttcggcccgcgttaccgcgagctgattgacgaaatttttaatcatgatggcctcgcagaggacttctcactttatctgcacgcgccctgtgtcacggattcgtcactggcgcctgaaggttgcggcagttactatgtgttggcgccggtgccgcatttaggcaccgcgaacctcgactggacggttgaggggccaaaactacgcgaccgtatttttgcgtaccttgagcagcattacatgcctggcttacggagtcagctggtcacgcaccggatgtttacgccgtttgattttcgcgaccagcttaatgcctatcatggctcagccttttctgtggagcccgttcttacccagagcgcctggtttcggccgcataaccgcgataaaaccattactaatctctacctggtcggcgcaggcacgcatcccggcgcaggcattcctggcgtcatcggctcggcaaaagcgacagcaggtttgatgctggaggatctgatataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118008_sequence/1
type: Sequence
persistent identity: BBa K118008 sequence
display id: BBa_K118008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcaaccgcattatgatctgattctcgtgggggctggactcgcgaatggccttatcgccctgcgtcttcagcagcagcaacctgatatgcgtattttgcttatcgacgccgcaccccaggcgggcgggaatcatacgtggtcatttcaccacgatgatttgactgagagccaacatcgttggatagctccgctggtggttcatcactggcccgactatcaggtacgctttcccacacgccgtcgtaagctgaacagcggctacttttgtattacttctcagcgtttcgctgaggttttacagcgacagtttggcccgcacttgtggatggataccgcggtcgcagaggttaatgcggaatctgttcggttgaaaaagggtcaggttatcggtgcccgcgcggtgattgacgggcggggttatgcggcaaattcagcactgagcgtgggcttccaggcgtttattggccaggaatggcgattgagccacccgcatggtttatcgtctcccattatcatggatgccacggtcgatcagcaaaatggttatcgcttcgtgtacagcctgccgctctcgccgaccagattgttaattgaagacacgcactatattgataatgcgacattagatcctgaatgcgcgcggcaaaatatttgcgactatgccgcgcaacagggttggcagcttcagacactgctgcgagaagaacagggcgccttacccattactctgtcgggcaatgccgacgcattctggcagcagcgccccctggcctgtagtggattacgtgccggtctgttccatcctaccaccggctattcactgccgctggcggttgccgtggccgaccgcctgagtgcacttgatgtctttacgtcggcctcaattcaccatgccattacgcattttgcccgcgagcgctggcagcagcagggctttttccgcatgctgaatcgcatgctgtttttagccggacccgccgattcacgctggcgggttatgcagcgtttttatggtttacctgaagatttaattgcccgtttttatgcgggaaaactcacgctgaccgatcggctacgtattctgagcggcaagccgcctgttccggtattagcagcattgcaagccattatgacgactcatcgttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118012_sequence/1
type: Sequence
persistent identity: BBa K118012 sequence
display id: BBa_K118012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: gagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K539119_sequence/1
type: Sequence
persistent identity: BBa K539119 sequence
display id: BBa_K539119_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcaaccgcattatgatctgattctcgtgggggctggactcgcgaatggccttatcgccctgcgtcttcagcagcagcaacctgatatgcgtattttgcttatcgacgccgcaccccaggcgggcgggaatcatacgtggtcatttcaccacgatgatttgactgagagccaacatcgttggatagctccgctggtggttcatcactggcccgactatcaggtacgctttcccacacgccgtcgtaagctgaacagcggctacttttgtattacttctcagcgtttcgctgaggttttacagcgacagtttggcccgcacttgtggatggataccgcggtcgcagaggttaatgcggaatctgttcggttgaaaaagggtcaggttatcggtgcccgcgcggtgattgacgggcggggttatgcggcaaattcagcactgagcgtgggcttccaggcgtttattggccaggaatggcgattgagccacccgcatggtttatcgtctcccattatcatggatgccacggtcgatcagcaaaatggttatcgcttcgtgtacagcctgccgctctcgccgaccagattgttaattgaagacacgcactatattgataatgcgacattagatcctgaatgcgcgcggcaaaatatttgcgactatgccgcgcaacagggttggcagcttcagacactgctgcgagaagaacagggcgccttacccattactctgtcgggcaatgccgacgcattctggcagcagcgccccctggcctgtagtggattacgtgccggtctgttccatcctaccaccggctattcactgccgctggcggttgccgtggccgaccgcctgagtgcacttgatgtctttacgtcggcctcaattcaccatgccattacgcattttgcccgcgagcgctggcagcagcagggctttttccgcatgctgaatcgcatgctgtttttagccggacccgccgattcacgctggcgggttatgcagcgtttttatggtttacctgaagatttaattgcccgtttttatgcgggaaaactcacgctgaccgatcggctacgtattctgagcggcaagccgcctgttccggtattagcagcattgcaagccattatgacgactcatcgttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61048_sequence/1
type: Sequence
persistent identity: BBa J61048 sequence
display id: BBa_J61048_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccggcttatcggtcagtttcacctgatttacgtaaaaacccgcttcggcgggtttttgcttttggaggggcagaaagatgaatgactgtccacgacgctatacccaaaagaaa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: Chris J. Myers
creator: James Alastair McLaughlin
ended at time: 2017-03-06T15:00:00.000Z