LOCUS BBa_K539151 4767 bp DNA linear 05-Nov-2024 DEFINITION promoter+crtEBI+RNA thermometer(FourU)+crtY+terminator ACCESSION BBa_K539151 VERSION BBa_K539151.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23103 RBS 44..47 /label=BBa_K118012 CDS 54..965 /label=BBa_I742151 misc_feature 974..976 /label=SacI RBS 977..983 /label=rbs RBS 974..983 /label=BBa_J15001 CDS 990..1919 /label=crtB coding sequence CDS 990..1922 /label=BBa_K118002 misc_feature 1931..1933 /label=SacI RBS 1934..1940 /label=rbs RBS 1931..1940 /label=BBa_J15001 misc_feature 2424..2429 /label=PstI site removed misc_feature 2835..2840 /label=PstI site removed CDS 1947..3425 /label=crtI coding sequence CDS 1947..3428 /label=BBa_K118003 misc_feature 44..3428 /label=BBa_K274100 misc_feature 1..3428 /label=BBa_K346090 RBS 3483..3488 /label=rbs misc_feature 3459..3464 /label=scar adaptation stem_loop 3437..3454 /label=stem_loop stem_loop 3460..3488 /label=predicted stem-loop extending to one position before start codon misc_feature 3437..3488 /label=BBa_K115002 CDS 3495..4643 /label=crtY coding sequence CDS 3495..4646 /label=BBa_K118008 CDS 3495..4643 /label=crtY coding sequence misc_feature 3495..4646 /label=BBa_K118008 CDS 3495..4646 /label=BBa_K539119 terminator 4655..4767 /label=BBa_J61048 misc_feature 3437..4767 /label=BBa_K539138 ORIGIN 1 ctgatagcta gctcagtcct agggattatg ctagctacta gaggaggtac tagatgacgg 61 tctgcgcaaa aaaacacgtt catctcactc gcgatgctgc ggagcagtta ctggctgata 121 ttgatcgacg ccttgatcag ttattgcccg tggagggaga acgggatgtt gtgggtgccg 181 cgatgcgtga aggtgcgctg gcaccgggaa aacgtattcg ccccatgttg ctgttgctga 241 ccgcccgcga tctgggttgc gctgtcagcc atgacggatt actggatttg gcctgtgcgg 301 tggaaatggt ccacgcggct tcgctgatcc ttgacgatat gccctgcatg gacgatgcga 361 agctgcggcg cggacgccct accattcatt ctcattacgg agagcatgtg gcaatactgg 421 cggcggttgc cttgctgagt aaagcctttg gcgtaattgc cgatgcagat ggcctcacgc 481 cgctggcaaa aaatcgggcg gtttctgaac tgtcaaacgc catcggcatg caaggattgg 541 ttcagggtca gttcaaggat ctgtctgaag gggataagcc gcgcagcgct gaagctattt 601 tgatgacgaa tcactttaaa accagcacgc tgttttgtgc ctccatgcag atggcctcga 661 ttgttgcgaa tgcctccagc gaagcgcgtg attgcctgca tcgtttttca cttgatcttg 721 gtcaggcatt tcaactgctg gacgatttga ccgatggcat gaccgacacc ggtaaggata 781 gcaatcagga cgccggtaaa tcgacgctgg tcaatctgtt aggcccgagg gcggttgaag 841 aacgtctgag acaacatctt cagcttgcca gtgagcatct ctctgcggcc tgccaacacg 901 ggcacgccac tcaacatttt attcaggcct ggtttgacaa aaaactcgct gccgtcagtt 961 aataatacta gagctcaagg aggtactaga tgaataatcc gtcgttactc aatcatgcgg 1021 tcgaaacgat ggcagttggc tcgaaaagtt ttgcgacagc ctcaaagtta tttgatgcaa 1081 aaacccggcg cagcgtactg atgctctacg cctggtgccg ccattgtgac gatgttattg 1141 acgatcagac gctgggcttt caggcccggc agcctgcctt acaaacgccc gaacaacgtc 1201 tgatgcaact tgagatgaaa acgcgccagg cctatgcagg atcgcagatg cacgaaccgg 1261 cgtttgcggc ttttcaggaa gtggctatgg ctcatgatat cgccccggct tacgcgtttg 1321 atcatctgga aggcttcgcc atggatgtac gcgaagcgca atacagccaa ctggatgata 1381 cgctgcgcta ttgctatcac gttgcaggcg ttgtcggctt gatgatggcg caaatcatgg 1441 gcgtgcggga taacgccacg ctggaccgcg cctgtgacct tgggctggca tttcagttga 1501 ccaatattgc tcgcgatatt gtggacgatg cgcatgcggg ccgctgttat ctgccggcaa 1561 gctggctgga gcatgaaggt ctgaacaaag agaattatgc ggcacctgaa aaccgtcagg 1621 cgctgagccg tatcgcccgt cgtttggtgc aggaagcaga accttactat ttgtctgcca 1681 cagccggcct ggcagggttg cccctgcgtt ccgcctgggc aatcgctacg gcgaagcagg 1741 tttaccggaa aataggtgtc aaagttgaac aggccggtca gcaagcctgg gatcagcggc 1801 agtcaacgac cacgcccgaa aaattaacgc tgctgctggc cgcctctggt caggccctta 1861 cttcccggat gcgggctcat cctccccgcc ctgcgcatct ctggcagcgc ccgctctaat 1921 aatactagag ctcaaggagg tactagatga aaccaactac ggtaattggt gcaggcttcg 1981 gtggcctggc actggcaatt cgtctacaag ctgcggggat ccccgtctta ctgcttgaac 2041 aacgtgataa acccggcggt cgggcttatg tctacgagga tcaggggttt acctttgatg 2101 caggcccgac ggttatcacc gatcccagtg ccattgaaga actgtttgca ctggcaggaa 2161 aacagttaaa agagtatgtc gaactgctgc cggttacgcc gttttaccgc ctgtgttggg 2221 agtcagggaa ggtctttaat tacgataacg atcaaacccg gctcgaagcg cagattcagc 2281 agtttaatcc ccgcgatgtc gaaggttatc gtcagtttct ggactattca cgcgcggtgt 2341 ttaaagaagg ctatctaaag ctcggtactg tccctttttt atcgttcaga gacatgcttc 2401 gcgccgcacc tcaactggcg aaactgcaag catggagaag cgtttacagt aaggttgcca 2461 gttacatcga agatgaacat ctgcgccagg cgttttcttt ccactcgctg ttggtgggcg 2521 gcaatccctt cgccacctca tccatttata cgttgataca cgcgctggag cgtgagtggg 2581 gcgtctggtt tccgcgtggc ggcaccggcg cattagttca ggggatgata aagctgtttc 2641 aggatctggg tggcgaagtc gtgttaaacg ccagagtcag ccatatggaa acgacaggaa 2701 acaagattga agccgtgcat ttagaggacg gtcgcaggtt cctgacgcaa gccgtcgcgt 2761 caaatgcaga tgtggttcat acctatcgcg acctgttaag ccagcaccct gccgcggtta 2821 agcagtccaa caaactgcaa actaagcgca tgagtaactc tctgtttgtg ctctattttg 2881 gtttgaatca ccatcatgat cagctcgcgc atcacacggt ttgtttcggc ccgcgttacc 2941 gcgagctgat tgacgaaatt tttaatcatg atggcctcgc agaggacttc tcactttatc 3001 tgcacgcgcc ctgtgtcacg gattcgtcac tggcgcctga aggttgcggc agttactatg 3061 tgttggcgcc ggtgccgcat ttaggcaccg cgaacctcga ctggacggtt gaggggccaa 3121 aactacgcga ccgtattttt gcgtaccttg agcagcatta catgcctggc ttacggagtc 3181 agctggtcac gcaccggatg tttacgccgt ttgattttcg cgaccagctt aatgcctatc 3241 atggctcagc cttttctgtg gagcccgttc ttacccagag cgcctggttt cggccgcata 3301 accgcgataa aaccattact aatctctacc tggtcggcgc aggcacgcat cccggcgcag 3361 gcattcctgg cgtcatcggc tcggcaaaag cgacagcagg tttgatgctg gaggatctga 3421 tataataata ctagagggac aagcaatgct tgccttgaat agtaactttt gaatagtgat 3481 tcaggaggta ctagatgcaa ccgcattatg atctgattct cgtgggggct ggactcgcga 3541 atggccttat cgccctgcgt cttcagcagc agcaacctga tatgcgtatt ttgcttatcg 3601 acgccgcacc ccaggcgggc gggaatcata cgtggtcatt tcaccacgat gatttgactg 3661 agagccaaca tcgttggata gctccgctgg tggttcatca ctggcccgac tatcaggtac 3721 gctttcccac acgccgtcgt aagctgaaca gcggctactt ttgtattact tctcagcgtt 3781 tcgctgaggt tttacagcga cagtttggcc cgcacttgtg gatggatacc gcggtcgcag 3841 aggttaatgc ggaatctgtt cggttgaaaa agggtcaggt tatcggtgcc cgcgcggtga 3901 ttgacgggcg gggttatgcg gcaaattcag cactgagcgt gggcttccag gcgtttattg 3961 gccaggaatg gcgattgagc cacccgcatg gtttatcgtc tcccattatc atggatgcca 4021 cggtcgatca gcaaaatggt tatcgcttcg tgtacagcct gccgctctcg ccgaccagat 4081 tgttaattga agacacgcac tatattgata atgcgacatt agatcctgaa tgcgcgcggc 4141 aaaatatttg cgactatgcc gcgcaacagg gttggcagct tcagacactg ctgcgagaag 4201 aacagggcgc cttacccatt actctgtcgg gcaatgccga cgcattctgg cagcagcgcc 4261 ccctggcctg tagtggatta cgtgccggtc tgttccatcc taccaccggc tattcactgc 4321 cgctggcggt tgccgtggcc gaccgcctga gtgcacttga tgtctttacg tcggcctcaa 4381 ttcaccatgc cattacgcat tttgcccgcg agcgctggca gcagcagggc tttttccgca 4441 tgctgaatcg catgctgttt ttagccggac ccgccgattc acgctggcgg gttatgcagc 4501 gtttttatgg tttacctgaa gatttaattg cccgttttta tgcgggaaaa ctcacgctga 4561 ccgatcggct acgtattctg agcggcaagc cgcctgttcc ggtattagca gcattgcaag 4621 ccattatgac gactcatcgt taataatact agagccggct tatcggtcag tttcacctga 4681 tttacgtaaa aacccgcttc ggcgggtttt tgcttttgga ggggcagaaa gatgaatgac 4741 tgtccacgac gctataccca aaagaaa //